E8897

Manufactured by Merck Group

Sourced in Macao, United States

E8897 is a laboratory equipment product from Merck Group. It is designed for general scientific applications and functions as a precision measurement device. Technical specifications and detailed capabilities are available upon request.

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Lab products found in correlation

2 protocols using e8897

Immunoblotting and Microscopy Protocols

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Antibodies used for immunoblotting: rabbit anti-ErbB2 (29D8, Cell Signaling), mouse anti-ErbB2 (Ab-17, Thermo Fisher Scientific), rabbit anti-ErbB3 (Ab-1328, Sigma Aldrich, MO), rabbit anti-Ebp50 (SLC9A3R1, Sigma Aldrich), rabbit anti-Ebp50 (A310, Cell Signaling), mouse anti-ezrin (E8897, Sigma Aldrich), rabbit anti-radixin (HPA000763, Sigma Aldrich), rabbit anti-pERM (3149, Cell Signaling), mouse anti-GAPDH (Ab-9484, Abcam), mouse anti-pErk1/2 (9106, Cell Signaling), rabbit anti-pAkt (4058, Cell Signaling), mouse anti-Ubiquitin (P4G7, Covance). Antibodies used for microscopical analysis: rabbit anti-ErbB2 (29D8, Cell Signaling), mouse anti-ErbB2 (9G6, Santa Cruz), rabbit anti-pERM (3149, Cell Signaling), rabbit anti-ezrin (3145, Cell Signaling), mouse anti-ezrin (E8897, Sigma Aldrich), rabbit anti-radixin (HPA000763, Sigma Aldrich), mouse anti-radixin (1E12, Novus Biologicals), rabbit anti-Ebp50 (SLC9A3R1, Sigma Aldrich), mouse anti-Hsp90 (sc-13119, Santa Cruz), mouse anti-c-Cbl (Upstate). HEPES, bovine serum albumin, geldanamycin, lactacystin, chloroquine and n-octylglucopyranoside were purchased from Sigma-Aldrich. The small molecule inhibitor NSC668394 was purchased from Calbiochem, and the dynamin-inhibitor Dyngo4a was purchased from Fisher Scientific. The plasmids encoding GFP-ErbB2 and CFP-ErbB3 were kind gifts from Dr. B. van Deurs (University of Copenhagen, Denmark).

Asp N., Kvalvaag A., Sandvig K, & Pust S. (2016). Regulation of ErbB2 localization and function in breast cancer cells by ERM proteins. Oncotarget, 7(18), 25443-25460.

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Molecular Marker Analysis of Mutants

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Mouse antibody to PKCθ (IgG2a; #610090; BD Biosciences; San Jose, CA, USA), rabbit ZO-1 antibody (61-7300; Invitrogen, Carlsbad, CA, USA), mouse β-catenin antibody (IgG1; #sc-7963; Santa Cruz Biotech; Dallas, TX, USA), mouse antibody to ezrin (E8897; Sigma-Aldrich Corp., St. Louis, MO, USA), goat antibody to ezrin (sc-6409; Santa Cruz Biotech), rabbit antibody to glial fibrillary acidic protein (GFAP; DAKO, Carpinteria, CA, USA), mouse antibody to moesin/radixin (ab50007; Abcam, Cambridge, MA, USA), rabbit antibody to radixin (ab52495; Abcam), mouse antibody to rhodopsin (MS-1233-R7; Thermo Scientific, Waltham, MA, USA), rabbit antibody to blue opsin (AB5407; Chemicon International, Billerica, MA, USA), rabbit antibody to red/green opsin (AB5405; Millipore, Billerica, MA, USA), mouse antibody to E-cadherin (610181; BD Transduction, San Jose, CA, USA), rabbit antibody to pan-cadherin (ab6529-200; Abcam), rabbit antibody to α1 catenin (2028-1; Epitomics, Burlingame, CA, USA), rabbit phospho-ezrin/radixin/moesin (ERM) antibody (#3149; Cell Signaling, Danvers, MA, USA), and rabbit occludin antibody (#71-1500; Invitrogen) were used for marker analyses of the mutant. The loading control sampler kit (#4670; Cell Signaling) included horseradish peroxidase (HRP)-conjugated β-tubulin, β-actin, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibodies.

Ji X., Liu Y., Hurd R., Wang J., Fitzmaurice B., Nishina P.M, & Chang B. (2016). Retinal Pigment Epithelium Atrophy 1 (rpea1): A New Mouse Model With Retinal Detachment Caused by a Disruption of Protein Kinase C, θ. Investigative Ophthalmology & Visual Science, 57(3), 877-888.

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