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Antibodies against dnmt1 and dntm3α

Manufactured by Cell Signaling Technology

Antibodies against DNMT1 and DNMT3α are laboratory reagents used to detect and analyze the expression of these proteins in biological samples. DNMT1 and DNMT3α are DNA methyltransferase enzymes involved in the regulation of gene expression. These antibodies can be used in various applications, such as Western blotting, immunohistochemistry, and immunofluorescence, to study the role of these proteins in cellular processes.

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2 protocols using antibodies against dnmt1 and dntm3α

1

CDDO-Me Induced Epigenetic Modifications

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CDDO-Me was obtained from the National Cancer Institute, Bethesda, MD through the Rapid Access to Intervention Development Program. A 100 mM stock solution of CDDO-Me was prepared in DMSO, which was subsequently diluted in tissue culture medium to obtain the working concentrations. Antibodies against PARP-1, NF-κB (p65), Sp1, c-Myc and β-actin were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA). Anti-hTERT and p-TERT (Ser824) antibodies were obtained from-Abcam Inc. (Cambridge, MA). Antibodies against DNMT1 and DNTM3α were from Cell Signaling (Danvers, MA). Anti-acetylated histone H3 at lysine 9 (ac-H3K9), anti- acetylated histone H4 (ac-H4), anti-histone dimethyl-H3 lysine 4 (di-me-H3K4) and anti-trimethy-H3 lysine 9 (ac-tri-me-H3K9) were purchased from Millipore (Temecula, CA). Annexin V-FITC apoptosis detection kit II was obtained from BD Pharmingen (San Diego, CA, USA) and TRAPeze telomerase detection kit was purchased from Millipore (Millipore, Temecula, CA).
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2

CDDO-Me Induced Epigenetic Modifications

Check if the same lab product or an alternative is used in the 5 most similar protocols
CDDO-Me was obtained from the National Cancer Institute, Bethesda, MD through the Rapid Access to Intervention Development Program. A 100 mM stock solution of CDDO-Me was prepared in DMSO, which was subsequently diluted in tissue culture medium to obtain the working concentrations. Antibodies against PARP-1, NF-κB (p65), Sp1, c-Myc and β-actin were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA). Anti-hTERT and p-TERT (Ser824) antibodies were obtained from-Abcam Inc. (Cambridge, MA). Antibodies against DNMT1 and DNTM3α were from Cell Signaling (Danvers, MA). Anti-acetylated histone H3 at lysine 9 (ac-H3K9), anti- acetylated histone H4 (ac-H4), anti-histone dimethyl-H3 lysine 4 (di-me-H3K4) and anti-trimethy-H3 lysine 9 (ac-tri-me-H3K9) were purchased from Millipore (Temecula, CA). Annexin V-FITC apoptosis detection kit II was obtained from BD Pharmingen (San Diego, CA, USA) and TRAPeze telomerase detection kit was purchased from Millipore (Millipore, Temecula, CA).
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