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Uplansapo 1.4na

Manufactured by Olympus
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Sourced in Japan

The UPLanSApo 1.4NA is an objective lens for optical microscopy. It has a numerical aperture of 1.4, which allows for high-resolution imaging. The lens is designed for use with upright microscopes.

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Lab products found in correlation

Planapon, by Olympus (2 mentions) Deltavision core deconvolution microscope, by Cytiva (1 mentions) Deltavision core, by Cytiva (1 mentions) Spatial light modulator, by Hamamatsu Photonics (1 mentions)

3 protocols using uplansapo 1.4na

1

Deconvolution Microscopy Imaging Protocol

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All images were acquired on a Deltavision Core deconvolution microscope (Applied Precision) using a 60× 1.42NA PlanApo N (Olympus) or 100× UPLanSApo 1.4NA (Olympus) objective and a sCMOS camera. Twenty Z-sections with 0.2 μm spacing were acquired for each image with identical exposure conditions within each experiment. For presentation in figures, representative images were deconvolved (where indicated), followed by generation of maximum intensity projections of 5–10 Z-sections, which were scaled identically for all experimental conditions.
Xi L., Schmidt J.C., Zaug A.J., Ascarrunz D.R, & Cech T.R. (2015). A novel two-step genome editing strategy with CRISPR-Cas9 provides new insights into telomerase action and TERT gene expression. Genome Biology, 16, 231.
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2

Deconvolution Microscopy Imaging Protocol

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All images were acquired on a Deltavision Core deconvolution microscope (Applied Precision, GE Healthcare) using a 60× 1.42NA PlanApo N (Olympus, Japan) or 100× UPLanSApo 1.4NA (Olympus) objective and a sCMOS camera. 20 Z-sections with 0.2 µm spacing were acquired for each image with identical exposure conditions within each experiment. For analysis, maximum intensity projections were generated and scaled identically. For presentation in figures, representative images were deconvolved (where indicated), followed by generation of maximum intensity projections of 10 Z-sections, which were scaled identically for all experimental conditions.
Schmidt J.C., Dalby A.B, & Cech T.R. (2014). Identification of human TERT elements necessary for telomerase recruitment to telomeres. eLife, 3, e03563.
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3

Multimodal Imaging of Tight Junction Proteins

Cited 1 time
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We used stimulated emission depletion (STED) imaging and expansion microscopy. For STED, 640- and 561-nm diode excitation lasers, a 775-nm STED laser, all pulsed at 40 MHz, and a 100x (Olympus UPlanSApo, 1.4NA) were utilized. A spatial light modulator (Hamamatsu) was used to produce either a doughnut-shaped (2D-STED) or a top-hat (3D-STED) phase mask, shaping different depletion beams without changing the optical setup. To reduce photobleaching at an optimal signal to noise ratios we employed DyMIN® adaptive illumination.
For expansion microscopy, after immunofluorescence staining, gelation, digestion and expansion were performed as described before77 (link). Notably, we extended incubation time in monomer solution to 45 min, gelation time to 2.5 h and digestion was performed overnight. Images were taken with an HC PL APO CS2 40x/1.10 water objective. Expansion microscopy was used for qualitative representation of occludin and ZO-1 morphology.
Wenzel J., Lampe J., Müller-Fielitz H., Schuster R., Zille M., Müller K., Krohn M., Körbelin J., Zhang L., Özorhan Ü., Neve V., Wagner J.U., Bojkova D., Shumliakivska M., Jiang Y., Fähnrich A., Ott F., Sencio V., Robil C., Pfefferle S., Sauve F., Coêlho C.F., Franz J., Spiecker F., Lembrich B., Binder S., Feller N., König P., Busch H., Collin L., Villaseñor R., Jöhren O., Altmeppen H.C., Pasparakis M., Dimmeler S., Cinatl J., Püschel K., Zelic M., Ofengeim D., Stadelmann C., Trottein F., Nogueiras R., Hilgenfeld R., Glatzel M., Prevot V, & Schwaninger M. (2021). The SARS-CoV-2 main protease Mpro causes microvascular brain pathology by cleaving NEMO in brain endothelial cells. Nature Neuroscience, 24(11), 1522-1533.
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