WT and mutation p53 plasmids (R175H, R249S, R273H, and R280K) were reported previously51 (link),52 (link) and recloned into pEF1a lentiviral vector and packaged with 293T cells. Lentiviruses expressing WT and mutant p53 were used for the infection of UMSCC1 cells, followed by selection with puromycin. shRNA-control and shRNA-GSTP1 were purchased from Thermo Scientific (Lafayette, CO), packaged with lentiviral expression system, and applied for the transduction.
Shrna gstp1
Manufactured by Thermo Fisher Scientific
ShRNA-GSTP1 is a laboratory tool used for gene silencing studies. It targets the GSTP1 gene and can be used to investigate its function. The product consists of a vector containing a short hairpin RNA (shRNA) sequence designed to reduce GSTP1 expression.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using shrna gstp1
1
p53 Mutant Plasmid Expression
2
p53 Mutant Plasmid Expression
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