3 ma s2767

Manufactured by Selleck Chemicals

Sourced in United States

3-MA (S2767) is a chemical compound that functions as an autophagy inhibitor. It is commonly used in laboratory research settings to study cellular processes related to autophagy.

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Lab products found in correlation

Mg132 s2619, by Selleck Chemicals (2 mentions) Resazurin, by Solarbio (1 mentions) Myricetin, by Solarbio (1 mentions) Akt 4691, by Cell Signaling Technology (1 mentions) Rapamycin 9904, by Cell Signaling Technology (1 mentions) Goat anti mouse igg 7076, by Cell Signaling Technology (1 mentions) Goat anti rabbit igg 7074, by Cell Signaling Technology (1 mentions) Ly294002 9901, by Cell Signaling Technology (1 mentions) Ab52897, by Abcam (1 mentions) Doxycycline, by Merck Group (1 mentions) L7543, by Merck Group (1 mentions) Sc 130300, by Santa Cruz Biotechnology (1 mentions) Rapamycin s1039, by Selleck Chemicals (1 mentions) Hanks balanced salt solution (hbss), by Thermo Fisher Scientific (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Anti sirt1, by Abcam (1 mentions) Anti beclin1, by Abcam (1 mentions) Chloroquine c6628, by Merck Group (1 mentions) Poly 1 c p9582, by Merck Group (1 mentions) Dmem f12 1 1 medium, by Thermo Fisher Scientific (1 mentions)

8 protocols using 3 ma s2767

Myricetin Modulates Autophagy and Apoptosis Pathways

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Myricetin, Hoechst Staining Kit (G3680) and resazurin were purchased from Solarbio Life Sciences (Beijing, China). 3-MA (S2767) was purchased from Selleck Chemicals (Houston, TX, USA). The primary antibodies included Bax (5023S), Bcl-2 (15071S), Microtubule-associated protein1 light chain 3 (LC-3) I/II (12741S), Beclin-1 (3495S), Phosphatidyl inositide 3-kinase (PI3K) (13666S), phospho-PI3K (4228S), phospho-Akt (4060S), Akt (2920S), Mammalian target of rapamycin (mTOR) (2983S), phospho-mTOR (5536S) were purchased from Cell Signaling (MA, USA). β-actin (60008–1) was purchased from ProteinTech Group (Wuhan, China). Horseradish peroxidase-conjugated secondary antibodies (ZB-2301, ZB-2305) were purchased from ZSGB-Bio (Beijing, China).

Zhu M.L., Zhang P.M., Jiang M., Yu S.W, & Wang L. (2020). Myricetin induces apoptosis and autophagy by inhibiting PI3K/Akt/mTOR signalling in human colon cancer cells. BMC Complementary Medicine and Therapies, 20, 209.

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Autophagy Regulation via mTOR Pathway

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HBSP was synthesized at Shanghai Ketai Biotechnology. Rapamycin (9904) and Ly294002 (9901), and antibodies against LC3 (2775), Beclin1 (3495), β-actin (4967), phospho-mTOR (5536), mTOR (2983), p-Akt (4060), and Akt (4691) were purchased from Cell Signaling Technology. Antibodies against HIF-1α (ab82832) was purchaded from Abcam. Goat anti-rabbit IgG (7074) and goat anti-mouse IgG (7076) were purchased from Cell Signaling Technology. We purchased 3-MA (S2767) from Selleck Chemicals. Ad-mRFP-GFP-LC3 adenoviral was purchaded from DesignGene (Shanghai).

Tan R., Tian H., Yang B., Zhang B., Dai C., Han Z., Wang M., Li Y., Wei L., Chen D., Wang G., Yang H., He F, & Chen Z. (2018). Autophagy and Akt in the protective effect of erythropoietin helix B surface peptide against hepatic ischaemia/reperfusion injury in mice. Scientific Reports, 8, 14703.

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Investigating Autophagy and MMP12 in Cells

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Antibodies against ACTB (1:1000 dilution, sc-130300, mouse monoclonal, Santa Cruz Biotechnology, Dallas, TX, USA), LC3B (1:1000 dilution, L7543, rabbit polyclonal, Sigma-Aldrich, St. Louis, MO, USA), SQSTM1 (1:1000 dilution, 4814, rabbit monoclonal, Cell Signaling Technology, Danvers, MA, USA), MMP12 (1:2000 dilution, ab52897, rabbit monoclonal, Abcam, Cambridge, UK) were used. Spautin-1 (S7888) and 3-MA (S2767) were from Selleck (Houston, TX, USA). Doxycycline was purchased from Sigma-Aldrich (1226003, St. Louis, MO, USA). Mouse Elastin Peptide were from Elastin Products Company (CB573, Owensville, MO, USA).

Huang H.Q., Li N., Li D.Y., Jing D., Liu Z.Y., Xu X.C., Chen H.P., Dong L.L., Zhang M., Ying S.M., Li W., Shen H.H., Li Z.Y, & Chen Z.H. (2021). Autophagy Promotes Cigarette Smoke-Initiated and Elastin-Driven Bronchitis-Like Airway Inflammation in Mice. Frontiers in Immunology, 12, 594330.

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Cytokine Induction Assay Protocol

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Reagents were obtained from the following suppliers: 3‐MA (S2767), MG132 (S2619), and rapamycin (S1039) were purchased from Selleck. Poly(I:C) and poly(dA:dT) (Sigma‐Aldrich), DMSO (Sigma, D2650), and HBSS (Gibco, 1441787) were obtained from commercial sources. The enzyme‐linked immunosorbent assay (ELISA) kits for human IFN‐β and IL6 were purchased from Thermo Fisher and Dakewe Biotech.

He X., Zhu Y., Zhang Y., Geng Y., Gong J., Geng J., Zhang P., Zhang X., Liu N., Peng Y., Wang C., Wang Y., Liu X., Wan L., Gong F., Wei C, & Zhong H. (2019). RNF34 functions in immunity and selective mitophagy by targeting MAVS for autophagic degradation. The EMBO Journal, 38(14), e100978.

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Molecular Mechanisms of Autophagy Regulation

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AS-IV (C41H68O14, molecular weight =784.97, purity by high-performance liquid chromatography [HPLC] ≥98%) was purchased from Sigma-Aldrich Co. (St Louis, MO, USA). SRT1720 (S1129), EX527 (S1541), rapamycin (S1039), and 3-MA (S2767) were obtained from Selleck Chemicals (Houston, TX, USA). Rabbit polyclonal anti-alpha smooth muscle actin (α-SMA), anti-fibronectin (FN), anti-collagen IV (Col IV), anti-LC3 II, anti-Beclin 1, anti-SIRT1, and anti-NF-κB p65 (acetyl K310) (Ac-p65) antibodies were purchased from Abcam (Cambridge, UK).

Wang X., Gao Y., Tian N., Zhu Z., Wang T., Xu J., Wu B, & Zhang N. (2018). Astragaloside IV represses high glucose-induced mesangial cells activation by enhancing autophagy via SIRT1 deacetylation of NF-κB p65 subunit. Drug Design, Development and Therapy, 12, 2971-2980.

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Viral Infection Assay in Fish Cells

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Epithelioma papulosum cyprini (EPC) and zebrafish embryonic fibroblast (ZF4) cells were maintained at 28°C in 5% CO₂ in medium 199 (Hyclone) and DMEM/F12 (1:1) medium (Invitrogen) supplemented with 10% FBS, 100 U/mL penicillin and 100 mg/mL streptomycin, respectively. Spring viremia of carp virus (SVCV) was propagated in EPC cells. MG132 (S2619) and 3-MA (S2767) were purchased from Selleck. Chloroquine (C6628) and poly I:C (P9582) were purchased from Sigma-Aldrich. Leptomycin B (9676S) was purchased from Cell Signaling Technology.

Wu X.M., Fang H., Zhang J., Bi Y.H, & Chang M.X. (2021). Histone H2A Nuclear/Cytoplasmic Trafficking Is Essential for Negative Regulation of Antiviral Immune Response and Lysosomal Degradation of TBK1 and IRF3. Frontiers in Immunology, 12, 771277.

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Autophagy-related protein analysis

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The primary antibodies used were as follows: anti-LC3B (ab192890), anti-SQTSM1 (ab109012), anti-BECN1 (ab207612), anti-ATG5 (ab108327), anti-LAMP1 (ab25630), anti-LGALS3 (ab2785), anti-CTSB (ab125067), anti-CTSL (ab6314), and bafilomycin A₁ (ab120497) were procured from Abcam. Anti-HMGB1 (10,829-1-AP), anti-CYCS (10,993-1-AP), anti-ACTB (20,536-1-AP), Alexa Fluor 594 (SA00006-4), Alexa Fluor 488 (SA00006-1), and horseradish peroxidase-conjugated secondary antibodies (SA00001-2 and SA00001-1) were procured from Proteintech Group. Rapamycin (R0395), chloroquine (C6628), leupeptin (L9783), GA (467,214–21-7), and rotenone (R8875) were procured from Sigma-Aldrich. 3-MA (S2767) and CA-074Me (S7420) were procured from Selleck. Z-FF-FMK (108,005–94-3) was procured from Santa Cruz Biotechnology.

Feng L., Liang L., Zhang S., Yang J., Yue Y, & Zhang X. (2021). HMGB1 downregulation in retinal pigment epithelial cells protects against diabetic retinopathy through the autophagy-lysosome pathway. Autophagy, 18(2), 320-339.

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Autophagy Evaluation in Cell Lines

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Rabbit anti-LC3 (L8918), mouse anti-alpha-tubulin (T6199) and mouse anti-FLAG^® clone M2 (F1804) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Rabbit anti-SQSTM1/p62 (PA5-20839) was from ThermoFisher Scientific (Waltham, MA, USA). Rabbit anti-procaspase-3 (#9665), rabbit anti-cleaved-caspase-3 (Asp-175, clone 5A1E) (#9664), mouse anti-procaspase-9 (#9508), rabbit anti-PARP (#9542), rabbit anti-cleaved-PARP (#5625), rabbit anti-Atg5 (#12964) were from Cell Signaling (Danvers, MA, USA). Mouse anti-cytochrome c (7H8) (sc-13560) was from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Mouse anti-GFP (Ab1218) was from Abcam (Cambridge, UK). Rabbit and mouse horseradish-peroxidase-conjugated secondary antibody were from Cell Signaling. FITC-conjugated secondary antibody Alexa Fluor 488, MitoTracker Orange CMTM Ros (mitochondrial selective dye) (M7510) and the LysoTracker Green DND-26 (lysosomal selective dye) (L7526) were acquired from Molecular Probes (ThermoFisher Scientific). Trehalose (T0167), rapamycin (R0395), chloroquine (CQ) (C6628), ammonium chloride (NH₄Cl) and docetaxel were purchased from Sigma-Aldrich. 3-MA (S2767) was obtained from Selleckchem (Munich, Germany).

Cristofani R., Montagnani Marelli M., Cicardi M.E., Fontana F., Marzagalli M., Limonta P., Poletti A, & Moretti R.M. (2018). Dual role of autophagy on docetaxel-sensitivity in prostate cancer cells. Cell Death & Disease, 9(9), 889.

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