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Axiophot

Manufactured by Olympus
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Sourced in Japan

The Axiophot is a high-performance microscope system designed for advanced imaging applications. It features a modular design that allows for customization and integration of various imaging techniques, such as brightfield, darkfield, phase contrast, and fluorescence microscopy. The Axiophot's core function is to provide a versatile and reliable platform for detailed observation and analysis of samples.

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Lab products found in correlation

Fluoview fv1000, by Olympus (1 mentions) Fluoview fv1000 confocal, by Olympus (1 mentions) Dp50 camera system, by Olympus (1 mentions) Om d e m5, by Olympus (1 mentions) Axiophot, by Zeiss (1 mentions) Szx16, by Olympus (1 mentions)

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Axiophot microscope

6 protocols using axiophot

1

Brightfield and Phase Contrast Imaging

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Brightfield images of spheroids were acquired on a Leica DMi1 inverted microscope with a 4x objective or an EVOS FL inverted microscope with a 20x objective. Phase contrast images were acquired on a Zeiss Axiophot using an Olympus DP72 camera attachment. The objective was a 20x plan neofluor objective (dry) with a phase 2 condenser.
Lee M.A., Bergdorf K.N., Phifer C.J., Jones C., Byon S.Y., Sawyer L.M., Bauer J, & Weiss V.L. (2020). Novel three dimensional cultures provide insights into thyroid cancer behavior. Endocrine-related cancer, 27(2), 111-121.
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2

Quantifying Retinal Cell Proliferation

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Slides were examined with a Zeiss Axiophot, Observer.Z1 microscope or an Olympus FluoView FV1000 confocal imaging system. Cell counts were determined by counting fluorescently labeled BrdU+ or GFP+ cells in retinal sections visualized using fluorescent microscopy. All experiments were done in triplicate or more and repeated at least twice. Experimenters were blind to the animal treatments until after data analysis. ANOVA with Bonferroni/Dunn post hoc t-test was used for multiple comparisons and a two-tailed unpaired Student’s t test for single comparison (experimental vs control group). When n=3, the Mann-Whitney test was employed for single comparison between control and experimental group.
Zhao X.F., Wan J., Powell C., Ramachandran R., Myers MG J.r, & Goldman D. (2014). Leptin and IL-6 Family Cytokines Synergize to Stimulate Müller Glia Reprogramming and Retina Regeneration. Cell reports, 9(1), 272-284.
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3

Fluorescence Imaging Protocol

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Imagines were captured by a Zeiss Axiophot fluorescence microscope or an Olympus FluoView FV1000 confocal microscope.
Wan J., Zhao X.F., Vojtek A, & Goldman D. (2014). Retinal injury, growth factors and cytokines converge on β-catenin and pStat3 signaling to stimulate retina regeneration. Cell reports, 9(1), 285-297.
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4

Immunofluorescence Staining Protocol

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After deparaffinization, rehydration through a series of xylene and ethanol washes, and 3 washes with phosphate-buffered saline, sections were incubated with 2 μg/mL anti-e-cadherin and anti-TH primary antibodies diluted in a 0.5% solution of bovine serum albumin in PBS overnight at 4°C. The slides were washed 3 times with PBS and incubated for 1 hour at room temperature with fluorescein isothiocyanate (FITC) secondary antibodies. After 3 washes with PBS, the slides were mounted with glycerol and coverslips. The samples were examined under a fluorescence microscope (Zeiss Axiophot) equipped with an Olympus DP 50 camera system.
Cataldi S., Codini M., Hunot S., Légeron F.P., Ferri I., Siccu P., Sidoni A., Ambesi-Impiombato F.S., Beccari T., Curcio F, & Albi E. (2016). e-Cadherin in 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine-Induced Parkinson Disease. Mediators of Inflammation, 2016, 3937057.
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5

Cell Density Quantification in Daphnia Head

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To investigate the cell density pattern, we determined cell numbers in different head areas, by counting the DAPI-labeled nuclei in squares of 50 × 50 μm. A minimum of 7 and a maximum of 10 individuals were examined per sampling point and treatment.
Specimens were photographed under a fluorescence microscope (Zeiss, Axiophot) (XC10 Olympus + CellSens) with 40× magnification (Plan Apo oil immersion, Olympus) in each area of interest. Afterwards, 50 × 50 μm squares according to the scale of the images were overlayed and nuclei within this square were counted using FIJI (Schindelin et al., 2012 ). We focused on three areas (ventral, cranial, dorsal; displayed in Figure 1E, F) of the Daphnia head to conduct the cell counts. Positions were identified using landmarks, so that the anterior margin of the square in the ventral area was adjusted by superimposing a vertical line at the posterior eye margin. The upper margin of the square was delimited by the top of the head. The square in the cranial area was set above the base of the 2nd antenna, with the upper margin being defined by the top of the head. The lower margin of the square in the dorsal area was defined by a horizontal line through the middle of the compound eye. The posterior verge was set at the dorsal margin of the Daphnia head (Figure 1E, F).
Graeve A., Huster J., Görl D., Ioannidou I., Gómez R, & Weiss L.C. (2022). Distinct cell proliferation patterns underlying the development of defensive crests in Daphnia longicephala. Heliyon, 8(9), e10513.
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6

Specimen Collection and Microscopy Analysis

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The specimens studied were collected by beating and were freshly killed and stored in 95% ethanol. Examination was made by using a stereoscopic microscope (SZX16: Olympus, Japan) or a compound microscope (Axiophot: Carl Zeiss Microscopy, Germany). The photographs were taken by using a digital camera (OM-D E-M5: Olympus, Japan) attached to SZX 16 (habitus) or Axiophot (forewing). Terminology followed Yoshizawa et al (2014) .
Yoshizawa K., Souza-Silva M, & Ferreira R.L. (2017). Disjunct Occurrence of Trichadenotecnum s.str. in Southeastern Brazil (Psocodea: "Psocoptera": Psocidae), with Description of a New Species. Neotropical entomology, 46(2).
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