Vitek 2 ast gn80 test kit cards

The antimicrobial susceptibility test was evaluated using the minimum inhibitory concentration (MIC)-based automated system, the VITEK^® 2 AST-GN80 test kit cards, and VITEK^® 2 COMPACT machine (bioMérieux, Marcy l’Etoile, France) [31 ,32 (link)]. The antimicrobial resistance was determined using an advanced Expert System™ based on the global Clinical and Laboratory Standards Institute guidelines and natural resistance guidelines.
Drug that used for MIC (μg/mL) composed of benzylpenicillin (0.125, 0.25, 1, 2, 8, and 64), oxacillin (0.5, 1, and 2), cefalotin (4, 8, and 32), cefovecin, ceftiofur (0.5, 2, and 4), kanamycin (32, 64, and 128), enrofloxacin (1 and 2), marbofloxacin (0.5 and 2), pradofloxacin (0.125, 0.5, and 2), erythromycin (0.25, 0.5, and 2), clindamycin (CM 0.5 and CM/E 0.25/0.5), doxycycline (0.25, 1, 2, and 4), tetracycline (0.5, 1, and 2), nitrofurantoin (16, 32, and 64), chloramphenicol (2, 8, and 16), and sulfamethoxazole/trimethoprim (152/8, 304/16, and 608/32).

All samples were examined for AMR patterns through a minimum inhibitory concentration (MIC) - based automated system, the VITEK^® 2 AST-GN80 test kit cards, and the VITEK^® 2 COMPACT machine (bioMérieux) [20 (link), 21 (link)]. The MIC results were interpreted using an advanced Expert System™ based on the global Clinical and Laboratory Standards Institute and natural resistance guidelines, as in a previous study [7 (link)]. The following antimicrobial drugs were included in the VITEK^® 2 AST-GN80 test: Benzylpenicillin, oxacillin, cefalotin (CEF), cefovecin (CEC), ceftiofur (CET), kanamycin (KAN), enrofloxacin (ENR), marbofloxacin (MAR), pradofloxacin, erythromycin, clindamycin (CLI), doxycycline, tetracycline (TCN), nitrofurantoin (NFT), chloramphenicol, and sulfamethoxazole/trimethoprim (SXT). Three AMR genes were re-examined, namely mecA, mecC, and blaZ [21 (link)–23 (link)]. Polymerase chain reaction (PCR) was performed with the primers used in a previous study [7 (link)]. Staphylococcal chromosomal cassette mec gene detection was performed through multiplex PCR [24 (link)].