Creatinine jaff gen 2

Blood samples were taken from all subjects after a 12-h fasting period to measure serum concentrations of glucose, insulin, SUA and creatinine. Commercial kits, normally used for routine examinations of patients, were employed for all analyses. In detail: enzymatic method with hexokinase Glucose HK Gen.3 Cobas Roche, for glucose assay; immuno Assay in ElectroChemiLuminescence Elecsys Insulin Cobas Roche for insulin assay; colorimetric enzymatic test Uric Acid 2 Cobas Roche for uric acid assay; colorimetric kinetic test based on the Jaffé method Creatinine Jaffé Gen.2 Cobas Roche for creatinine assay. HOMA-index was calculated by dividing the product of serum insulin (μU/ml) and serum glucose (mmol/L) by 22.5 (22 (link)). Glomerular filtration rate was estimated (eGFR) by means of the Schwartz formula using serum creatinine and height measurements and a k constant of 0.55 (23 (link)).

Blood samples were taken from all subjects after a 12-h fasting period in order to measure serum concentrations of total cholesterol, high density lipoprotein (HDL), Triglycerides, glucose, insulin, uric acid and creatinine. Commercial kits, normally used for routine examinations of patients, were employed for all analyses. In detail: enzymatic colorimetric test Cholesterol Gen.2 Cobas Roche, for total cholesterol assay; colorimetric enzymatic test in homogeneous phase HDL-Cholesterol Gen.4 Cobas Roche, for HDL cholesterol; enzymatic colorimetric test Triglycerides Cobas Roche, for Triglycerides assay; enzymatic method with hexokinase Glucose HK Gen.3 Cobas Roche, for glucose assay; immunoassay in ElectroChemiLuminescence Elecsys Insulin Cobas Roche, for insulin assay; colorimetric enzymatic test Uric Acid 2 Cobas Roche, for uric acid assay; and the colorimetric kinetic test based on the Jaffé method, Creatinine Jaffé Gen.2 Cobas Roche, for creatinine assay. LDL cholesterol was calculated using Friedewald’s formula, LDL cholesterol = total cholesterol − [HDL cholesterol + (triglyceridemia/5)]. HOMA index was calculated by dividing the product of serum insulin (µU/mL) and serum glucose (mmol/L) by 22.5 [17 (link)]. Glomerular filtration rate was estimated (eGFR) by means of the Schwartz formula using serum creatinine and height measurements and a k constant of 0.55 [18 (link)].

Mice were placed for 24 h in metabolic cages for urine collection. Retroorbital blood sampling was performed subsequently. Blood and urine samples were analyzed at the Fribourg Cantonal Hospital (Laboratoire HFR) using an ion-selective electrode indirect method for the detection of Na⁺, K⁺, Cl^-, Cat. No. 0588392001 (ISE indirect Na⁺, K⁺, C^- for Gen.2, Roche, Basel Switzerland); quantitative determination of creatinine, kit CREJ2- Cat. No. 04810716 190 (Creatinine Jaffé Gen.2, Roche, Basel Switzerland) and; quantitative determination of urinary protein using TPUC3, Cat. No. 03333825 190 (Total Protein Urine/CSF Gen.3, Roche, Basel Switzerland) on instrument Cobas 6000 (Roche, Basel, Switzerland). Analysis was performed in a blinded fashion.