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Access assay

Manufactured by Beckman Coulter
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The Access Assay is an automated immunoassay system designed for diagnostic testing. It provides quantitative and qualitative measurements of various analytes in biological samples. The system utilizes chemiluminescent technology to perform a range of immunoassays.

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Lab products found in correlation

Radioimmunoassay, by Linco Research (4 mentions) Glucose oxidase method, by Yellow Springs Instruments (4 mentions) Radioimmunoassay, by Merck Group (2 mentions) Gas chromatographic mass spectrometry, by Thermo Fisher Scientific (2 mentions) Gas chromatography mass spectrometry, by Thermo Fisher Scientific (1 mentions) Glucose oxidase method, by Beckman Coulter (1 mentions) Taqman, by Thermo Fisher Scientific (1 mentions) Dipeptidyl peptidase 4 inhibitor, by Linco Research (1 mentions) Optimatm le 80 k, by Beckman Coulter (1 mentions)

10 protocols using access assay

1

Blood Glucose and Hormone Measurement

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Glucose concentrations were measured using a glucose oxidase method (Yellow Springs Instruments, Yellow Springs, OH). Plasma insulin was measured using a chemiluminescence assay (Access Assay; Beckman, Chaska, MN). Plasma glucagon and C-peptide were measured by Radio-Immunoassay (Linco Research, St. Louis, MO).
Varghese R.T., Man C.D., Laurenti M.C., Piccinini F., Sharma A., Shah M., Bailey K.R., Rizza R.A., Cobelli C, & Vella A. (2017). Performance of individually-measured vs population-based C-peptide kinetics to assess β-cell function in presence and absence of acute insulin resistance. Diabetes, obesity & metabolism, 20(3), 549-555.
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2

Measuring Plasma Glucose Metabolism

Cited 1 time
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Plasma samples were placed on ice, centrifuged at 4°C, separated, and stored at −20°C until assayed. Glucose concentrations were measured using a glucose oxidase method (Yellow Springs Instruments, Yellow Springs, OH, USA). Plasma insulin was measured using a chemiluminescence assay (Access Assay; Beckman Coulter, Chaska, MN, USA). Plasma glucagon and C-peptide were measured by Radio-Immunoassay (EMD Millipore, Billerica, MA, USA). Plasma [6,6-2H2] glucose and [1-13C] glucose enrichments were measured using gas chromatographic mass spectrometry (Thermoquest, San Jose, CA, USA) to simultaneously monitor the C-1 and C-2 and C-3 to C-6 fragments, as described by Beylot et al.23 (link) In addition, [6-3H] glucose specific activity was measured by liquid scintillation counting following deproteinization and passage over anion and cation exchange columns.22 (link)
Sathananthan M., Ikramuddin S., Swain J.M., Shah M., Piccinini F., Dalla Man C., Cobelli C., Rizza R.A., Camilleri M, & Vella A. (2014). The effect of vagal nerve blockade using electrical impulses on glucose metabolism in nondiabetic subjects. Diabetes, Metabolic Syndrome and Obesity: Targets and Therapy, 7, 305-312.
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3

Plasma Metabolite Measurement Protocol

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Plasma samples were placed on ice, centrifuged at 4°C, separated and then stored at −20°C until assayed. Glucose concentrations were measured using the glucose oxidase method (Yellow Springs Instruments, Yellow Springs, OH, USA). Plasma insulin was measured using a chemiluminescence assay (Access Assay; Beckman, Chaska, MN, USA). Plasma glucagon and C-peptide concentrations were measured by Radio-Immunoassay (Linco Research, St Louis, MO, USA). Plasma [6,6-2H2]glucose and [1-13C]glucose enrichments were measured using gas chromatographic MS (Thermoquest, San Jose, CA, USA) to simultaneously monitor C-1 plus C-2 and C-3 – C-6 fragments, as described by Beylot et al [23 (link)]. In addition, [6-3H]glucose specific activity was measured by liquid scintillation counting after deproteinisation and anion exchange and cation exchange chromatography.
Sharma A., Laurenti M.C., Man C.D., Varghese R.T., Cobelli C., Rizza R.A., Matveyenko A, & Vella A. (2017). Glucose metabolism during rotational shift-work in health-care workers. Diabetologia, 60(8), 1483-1490.
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4

Glucose and Isotope Tracer Analysis

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Plasma samples were placed on ice, centrifuged at 4°C, separated, and stored at −20°C until assay. Glucose concentrations were measured using a glucose oxidase method (Yellow Springs Instruments, Yellow Springs, OH). Plasma insulin was measured using a chemiluminescence assay with reagents obtained from Beckman (Access Assay; Beckman, Chaska, MN). Plasma glucagon and C-peptide were measured by radioimmunoassay using reagents supplied by Linco Research (St. Louis, MO). Plasma [6,6-2H2]glucose and [1-13C]glucose enrichments were measured using gas chromatography–mass spectrometry (Thermoquest, San Jose, CA) to simultaneously monitor the C-1, C-2, and C-3 to C-6 fragments, as described by Beylot et al. [30 (link)]. [6-3H]glucose specific activity was measured by liquid scintillation counting following deproteinization and passage over anion- and cation-exchange columns.
Adams J.D., Treiber G., Hurtado M.D., Laurenti M.C., Dalla Man C., Cobelli C., Rizza R.A, & Vella A. (2018). Increased Rates of Meal Absorption Do Not Explain Elevated 1-Hour Glucose in Subjects With Normal Glucose Tolerance. Journal of the Endocrine Society, 3(1), 135-145.
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5

Plasma Glucose and Hormone Analysis

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Plasma samples were placed on ice, centrifuged at 4°C, separated, and stored at −20°C until assayed. Glucose concentrations were measured from dorsal vein samples using a glucose oxidase method (Yellow Springs Instruments, Yellow Springs, OH). Plasma insulin was measured from hepatic vein samples using a chemiluminescence assay (Access Assay; Beckman, Chaska, MN). Plasma glucagon was measured from hepatic vein samples by Radioimmunoassay (Linco Research, St. Louis, MO).
Laurenti M.C., Arora P., Dalla Man C., Andrews J.C., Rizza R.A., Matveyenko A., Bailey K.R., Cobelli C, & Vella A. (2022). The relationship between insulin and glucagon concentrations in non‐diabetic humans. Physiological Reports, 10(13), e15380.
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6

Genotyping and Metabolic Biomarkers Analysis

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Genotyping of the rs7903146 SNP was done using Taqman (Applied Biosystems Inc., Foster City, CA). Blood samples from the OGTT were placed on ice, centrifuged at 4°C to separate plasma and stored at −20°C until assayed. Glucose and insulin concentrations were measured using a glucose oxidase method (Yellow Springs Instruments, Yellow Springs, OH) and a chemiluminescence assay (Access Assay; Beckman, Chaska, MN), respectively. Plasma free fatty acid (FFA) concentrations were measured as previously described [19 (link)]. We measured the concentrations of the C14:0, C16:0, C18:0 saturated fatty acids (SFA), the C16:1n-7, C18:1n-9 cis-monounsaturated fatty acids (MUFA), the C18:2n-6, C18:3n-3, C20:5n-3, C20:4n-6, C22:6n-3 polyunsaturated fatty acids (PUFA) and C16:1 trans-9 and C18:1 trans-9 trans-fatty acids. The percentages of fatty acids subgroups (%SFA, %MUFA, %PUFA) were also calculated.
Lu J., Varghese R.T., Zhou L., Vella A, & Jensen M.D. (2016). Glucose Tolerance and Free Fatty Acid Metabolism in Adults with Variations in TCF7L2 rs7903146. Metabolism: clinical and experimental, 68, 55-63.
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7

Isotopic Glucose Kinetics Measurement

Cited 1 time
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Plasma samples were placed on ice, centrifuged at 4°C, separated, and stored at −20°C until assayed. Glucose concentrations were measured using a glucose oxidase method (Yellow Springs Instruments, Yellow Springs, OH). Plasma insulin was measured using a chemiluminescence assay (Access Assay; Beckman, Chaska, MN). Plasma glucagon and C-peptide were measured by Radio-Immunoassay (Linco Research, St. Louis, MO). Collection tubes for GLP-1 had 100 μmol/L dipeptidyl peptidase-4 inhibitor (Linco Research, St. Louis, MO) added. Total GLP-1 concentrations were measured using a COOH-terminal assay (Linco Research). Plasma [6,6-2H2]glucose and [1-13C]glucose enrichments were measured using gas chromatographic mass spectrometry (Thermoquest, San Jose, CA) to simultaneously monitor the C-1 and C-2 and C-3 to C-6 fragments, as described by Beylot et al. (22 (link)). In addition, [6-3H]glucose specific activity was measured by liquid scintillation counting after deproteinization and passage over anion- and cation-exchange columns (20 (link)).
Shah M., Law J.H., Micheletto F., Sathananthan M., Dalla Man C., Cobelli C., Rizza R.A., Camilleri M., Zinsmeister A.R, & Vella A. (2014). Contribution of Endogenous Glucagon-Like Peptide 1 to Glucose Metabolism After Roux-en-Y Gastric Bypass. Diabetes, 63(2), 483-493.
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8

Plasma Biomarker Measurement Protocol

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All blood was immediately placed on ice after collection, centrifuged at 4°C, separated, and stored at −80°C until assay. Plasma glucose concentrations were measured using a Yellow Springs glucose analyzer. Plasma insulin concentrations were measured using a chemiluminescence assay (Access Assay, Beckman, Chaska, MN). Plasma C-peptide was measured using a 2-site immunenzymatic sandwich assay (Roche Diagnostics, Indianapolis, IN). Glucagon was measured using a two-site ELISA (Mercodia, Winston Salem, NC) in accordance with the manufacturer’s instructions.
Kohlenberg J.D., Laurenti M.C., Egan A.M., Schembri Wismayer D., Bailey K.R., Cobelli C., Dalla Man C, & Vella A. (2022). Differential Contribution of alpha- and beta-cell Dysfunction to Impaired Fasting Glucose and Impaired Glucose Tolerance. Diabetologia, 66(1), 201-212.
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9

Measurement of Glucose Metabolism Markers

Cited 1 time
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Plasma samples were placed on ice, centrifuged at 4°C, separated, and stored at −20°C until assayed. Glucose concentrations were measured using a glucose oxidase method (Yellow Springs Instruments, Yellow Springs, OH). Plasma insulin was measured using a chemiluminescence assay (Access Assay; Beckman, Chaska, MN). Plasma glucagon and C-peptide were measured by Radio-Immunoassay (EMD Millipore, Billerica, MA). Collection tubes for GLP-1 had 100 μM of DPP-4 inhibitor added. Total GLP-1 concentrations were measured using a C-terminal assay (EMD Millipore, Billerica, MA). Plasma [6,6-2H2] glucose and [1-13C] glucose enrichments were measured using gas chromatographic mass spectrometry (Thermoquest, San Jose, CA) to simultaneously monitor the C-1 and C-2 and C-3 to C-6 fragments, as described previously [19 (link)]. In addition, [6-3H] glucose specific activity was measured by liquid scintillation counting following deproteinization and passage over anion and cation exchange columns [18 (link)].
Shah M., Laurenti M.C., Man C.D., Ma J., Cobelli C., Rizza R.A, & Vella A. (2018). Contribution of Endogenous Glucagon-Like Peptide-1 to Changes in Glucose Metabolism and Islet Function in People with Type 2 Diabetes Four Weeks after Roux-en-Y Gastric Bypass (RYGB). Metabolism: clinical and experimental, 93, 10-17.
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10

Ultracentrifugation of Plasma for VLDL Extraction

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2 ml of plasma samples were placed in the bottom of an ultracentrifuge tube, covered with 3.6 mL of 1.006 g/mL density solution and centrifuged at 145,606 g for 19.5 h at 4°C in a Beckman ultracentrifuge (OptimaTM, LE-80K, Beckman Instruments, Spinco Division, Palo Alto, CA). Afterwards the top 2 cm of the tube, containing the VLDL particles were collected. The VLDL were extracted with the Dole solution (see above), the lipids fractionated and the TG’s hydrolysed, extracted, derivatized and measured by UPLC as described above.
Plasma glucose concentrations were measured using the glucose oxidase method (YSI, Yellow Springs, OH). A chemiluminescence assay with reagents (Access Assay; Beckman Coulter Inc., Chaska, MN) was used to measure insulin concentrations.
Ramos P., Bush N.C, & Jensen M.D. (2020). Sex and Depot Differences in Palmitoleic Acid Content of Human Blood and Fat. Lipids, 55(1), 63-72.
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