Total as160

Manufactured by Merck Group

The Total AS160 is a laboratory equipment designed for the analysis and quantification of proteins in biological samples. It utilizes advanced spectroscopic techniques to provide accurate and reliable results. The core function of the Total AS160 is to enable researchers and scientists to efficiently measure and characterize protein concentrations in their experiments.

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Lab products found in correlation

Total akt, by Cell Signaling Technology (2 mentions) Phospho tbc1d1 thr590, by Cell Signaling Technology (1 mentions) Total acetyl coa carboxylase acc, by Cell Signaling Technology (1 mentions) Antibodies against phospho as160 thr642, by Merck Group (1 mentions) Phospho acc ser79, by Merck Group (1 mentions) Phospho tbc1d1 ser237, by Merck Group (1 mentions) Hrp conjugated anti rabbit igg, by Thermo Fisher Scientific (1 mentions) Hrp conjugated anti sheep igg, by Merck Group (1 mentions) Antibodies against phospho akt ser473, by Cell Signaling Technology (1 mentions) Enhanced chemi luminescence (ecl), by GE Healthcare (1 mentions) Phospho akt thr308, by Cell Signaling Technology (1 mentions) Ecl plus, by GE Healthcare (1 mentions) Ecl prime, by GE Healthcare (1 mentions) Anti glut4antibody, by Bio-Rad (1 mentions) Phosphorylated as160, by Cell Signaling Technology (1 mentions) Phosphorylated akt ser473, by Cell Signaling Technology (1 mentions)

Close spelling variants of this product

AS160

2 protocols using total as160

Antibody-based Analysis of Insulin Signaling

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Antibodies against phospho‐Akt Ser⁴⁷³ (#9271), phospho‐Akt
Thr³⁰⁸ (#9275), phospho‐TBC1D1 Thr⁵⁹⁰ (#6927), total Akt
(#9272), and total acetyl CoA carboxylase (ACC, #3662) were purchased from Cell
Signaling Technology (Beverly, MA). Antibodies against phospho‐AS160 Thr⁶⁴²(#07‐802), phospho‐ACC Ser⁷⁹ (#07‐303),
phospho‐TBC1D1 Ser²³⁷ (#07‐2268), and total AS160
(#07‐741) were from Millipore (Temecula, CA). Anti‐phospho‐AS160
Ser⁵⁸⁸(#3028P2) was from Symansis Limited (Timaru, New Zealand). Anti‐GLUT4
antibody (#4670‐1704) was from Bio‐Rad AbD Serotec (Oxford, UK).
HRP‐conjugated anti‐rabbit IgG was from Biosource International (Camarillo, CA).
HRP‐conjugated anti‐sheep IgG was from Millipore. Enhanced chemiluminescence reagents
(ECL, ECL Plus, and ECL Prime) were obtained from GE Healthcare Life Sciences (Uppsala, Sweden). All
other reagents were obtained from Sigma (St. Louis, MO).

Iwabe M., Kawamoto E., Koshinaka K, & Kawanaka K. (2014). Increased postexercise insulin sensitivity is accompanied by increased AS160 phosphorylation in slow‐twitch soleus muscle. Physiological Reports, 2(12), e12162.

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Western Blot Analysis of Akt and AS160

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Gastrocnemius homogenates were resolved on SDS-PAGE gels, transferred to polyvinylidene fluoride membranes, and probed with phosphorylated Akt (Ser⁴⁷³; Cell Signaling), total Akt (Cell Signaling), phosphorylated AS160 (Ser⁵⁸⁸; Cell Signaling), and total AS160 (Millipore) antibodies.

Williams I.M., Otero Y.F., Bracy D.P., Wasserman D.H., Biaggioni I, & Arnold A.C. (2016). CHRONIC ANGIOTENSIN-(1–7) IMPROVES INSULIN SENSITIVITY IN HIGH-FAT FED MICE INDEPENDENT OF BLOOD PRESSURE. Hypertension, 67(5), 983-991.

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