Anti c5l2

Human DPSCs were purchased from Lonza Pharma & Biotech (Cat. # PT-5025). MEM-alpha, DMEM, PBS, fetal bovine serum, L-glutamine, and antibiotic–antimycotic solution were procured from Gibco™ Fisher Scientific (Waltham, MA, United States). Poly-D-Lysine (Bio-Coat™, 12 mm)-coated round German glass coverslips were purchased from Corning™ Fisher Scientific (Cat. # 354087; Waltham, MA, United States). RIPA buffer was from Cell Signaling (Danvers, MA, United States), the DMP-1 ELISA kit was from Invitrogen, Thermo Fisher Scientific (Waltham, MA, United States), and the DSPP ELISA kit was from MyBioSource (San Diego, CA, United States). Various antibodies were procured: anti-C5L2 was from BioLegend (San Diego, CA, United States), anti-DMP-1, anti-DSPP, and anti-β-actin were from Fisher Scientific (Waltham, MA, United States), and anti-STRO-1 was from Santa Cruz (Dallas, Texas, United States). Fluorescent secondary antibodies were from Life Technologies (Grand Island, NY, United States). Human recombinant TNFα was from Invitrogen, Thermo Fisher Scientific (Waltham, MA, United States), and a few other chemicals were from Fisher Chemical (Nazareth, PA, United States). C5L2 CRISPR/Cas9 KO plasmid human (sc-410578) and reagent system were purchased from Santa Cruz Biotechnology (Dallas, TX, United States).

Human dental pulp stem cells (DPSCs) were purchased from Lonza, Pharma & Biotech (Cat. # PT-5025). MEM-alpha, PBS, fetal bovine serum, L-glutamine, and Antibiotic–Antimycotic were procured from Gibco™ Fisher Scientific (Waltham, MA, USA). Poly-D-Lysine coated (BioCoat™, 12 mm) round German glass coverslips were purchased from Corning™ Fisher Scientific (Cat. # 354087; Waltham, MA, USA). RIPA buffer was from Cell Signaling Technology (Danvers, MA, USA) and BDNF ELISA kit from R&D System (Minneapolis, MN, USA). Various antibodies were procured: anti-C5L2 was from BioLegend (San Diego, CA, USA), rabbit anti-BDNF from NovusBio (Centennial, CO, USA), mouse anti-STRO-1, mouse anti-p38 and rabbit anti-pp38 from Santa Cruz (Dallas, Texas, USA). Fluorescent secondary antibodies were from Life Technologies (Grand Island, NY, USA). Lipopolysaccharide (LPS) was from Sigma-Aldrich (St. Louis, MO, USA; Cat.# L5418), and chemicals were from Fisher Chemical (Nazareth, PA, USA). siRNA targeting human C5L2, siRNA control and siRNA Reagent System were purchased from Santa Cruz Biotechnology (Dallas, TX, USA).