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Lambda 750 uv

Manufactured by PerkinElmer
Sourced in United States

The Lambda 750 UV is a high-performance UV-Vis spectrophotometer designed for accurate and reliable measurements. It features a wide wavelength range, high-resolution optics, and advanced data analysis capabilities.

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6 protocols using lambda 750 uv

1

Comprehensive Nanomaterial Characterization Protocol

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The EDS, TEM, and HRTEM images were captured using the high‐resolution field emission transmission electron microscope Talos F200s G2 operating at an acceleration voltage of 200 kV. DLS and ζ potential measurements were conducted at room temperature on a Malvern Zetasizer Nano ZS90 equipped with a solid‐state He‐Ne laser (λ = 633 nm). The crystal structure of CSP NPs was characterized with a Shimadzu XRD‐6000 X‐ray diffractometer equipped with Cu Kα1 radiation (λ = 0.15 406 nm). The UV–vis–NIR spectra were collected on a PerkinElmer Lambda 750 UV–vis–NIR spectrophotometer. FTIR was tested by the Thermo Scientific NICOLET iS 50 conventional Fourier infrared spectrometer. The TGA of Cur, CSP, and CSC NPs was characterized by the Thermo STA 499 F3 jupiter. The copper ions were determined by the inductively coupled plasma mass spectrometry (ICP‐MS, Thermo, ELEMENT 2).
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2

Characterization of HMnO2-MSC-TAT Nanoparticles

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The hydrodynamic diameters
and zeta potentials of the NPs were determined by DLS (Malvern Instruments,
UK). The morphology of HMnO2-MSC-TAT NPs was characterized
by TEM (FEI Tecnai G2, USA) and SEM (Zeiss Sigma 300, Germany). UV–vis
spectra were measured using a PerkinElmer Lambda 750 UV–vis–NIR
spectrophotometer. The surface area and pore size of HMnO2 were measured by a Surface Area and Porosity Analyzer (ASAP2460,
USA). The concentrations of Mn were determined by ICP-OES (ICPE 9000,
Japan). The protein contents in the membranes of the NPs were measured
by SDS-PAGE.
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3

Characterization of Polymer Memory Films

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The AFM images of the polymer films were scanned in tapping mode by atomic force microscope (AFM, Veeco Multimode V). The absorption spectrum of the polymer film was obtained using a Perkin Elmer Lambda 750 UV–visible near-infrared spectrophotometer with integrating sphere. The electrical characterization of the memory cells were carried out by a Keithley 2612 source meter and an Agilent 4155c semiconductor parameter analyzer at room temperature in ambient atmosphere.
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4

UV-Vis Spectroscopy for Graphene Oxide Dispersion

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Ultraviolet–visible spectrum can be generated when ultraviolet light, visible light and near-infrared light are absorbed by materials. The spectrum can be used to analyze the absorbance of the materials. The absorption degree is proportional to the concentration of the materials. The larger the absorbance of the solution, the more homogenous dispersion of GO in the solution [52 (link),53 (link),54 (link)]. As an indirect method, UV–Vis measures the degree of dispersion of GO in a non-quantitative manner. The dispersion of the materials are quantitatively reflected by the spectrum and better dispersion measured means higher absorbance. In this work, Lambda 750 UV–Vis spectrophotometer (PerkinElmer, Waltham, MA, USA, see in Figure 3) is used to evaluate the adsorption performance of GO aqueous solution.
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5

Multimodal Characterization of Nanoparticles

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Scanning electron microscopy (SEM; JSM-2100F, JEOL, Tokyo, Japan) was applied to characterize the nanoparticle morphology. Ultraviolet–visible (UV–Vis) spectra were measured with a PerkinElmer Lambda 750 UV/Vis/NIR spectrophotometer. Nanoparticle size and zeta potential were determined by a Malvern Zetasizer (ZEN3690, Malvern, UK) and Nano ZS90 (Malvern, UK). Surface area and pore size were measured by Surface Area and Porosity Analyzer (Micromeritics Instrument Corp. ASAP2050). The functional groups and chemical structure of the nanofibers were performed by Fourier transform infrared (FT-IR) spectroscopy (Nicolet iS50) in the wavenumber range of 4000–400 cm–1.
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6

Nanoparticle Characterization Techniques

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The morphology of nanoparticles was characterized by TEM (FEI Tecnai F20) operating at an acceleration voltage of 200 kV. The UV‐vis–NIR absorbance was recorded with a PerkinElmer Lambda 750 UV‐vis‐NIR spectrophotometer. The flow cytometry was characterized by FACMVerse (BD). The fluorescence of cells and tissues was analyzed by a scanning confocal microscope (FV1200, Olympus).
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