Epigallocatechin gallate (egcg)

Manufactured by Merck Group

Sourced in United States, Germany, China, Sao Tome and Principe, Spain, France, Canada, United Kingdom, Macao, India, Italy, Australia, Belgium, Israel, Denmark, Japan

EGCG is a laboratory reagent used in research applications. It is a bioactive compound extracted from green tea leaves. EGCG has demonstrated potential antioxidant and anti-inflammatory properties in scientific studies.

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Lab products found in correlation

Catechin, by Merck Group (69 mentions) Epicatechin, by Merck Group (68 mentions) Gallic acid, by Merck Group (68 mentions) Epigallocatechin, by Merck Group (54 mentions) Quercetin, by Merck Group (54 mentions) Epicatechin gallate, by Merck Group (45 mentions) Dimethyl sulfoxide (dmso), by Merck Group (40 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (40 mentions) Gallocatechin gallate, by Merck Group (33 mentions) Gallocatechin, by Merck Group (31 mentions) Caffeine, by Merck Group (27 mentions) 2 2 diphenyl 1 picrylhydrazyl (dpph), by Merck Group (24 mentions) Catechin gallate, by Merck Group (23 mentions) Rutin, by Merck Group (22 mentions) Kaempferol, by Merck Group (21 mentions) Myricetin, by Merck Group (20 mentions) Curcumin, by Merck Group (19 mentions) Caffeic acid, by Merck Group (18 mentions) Ferulic acid, by Merck Group (18 mentions) Resveratrol, by Merck Group (18 mentions)

602 protocols using epigallocatechin gallate (egcg)

Mouse Sepsis Cardiac Dysfunction Model

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Male C57BL/6 mice were purchased from the Experimental Animal Center of Nanjing Medical University (Nanjing, China). They were randomly classified into four groups (n = 6 each): (1) Control group; (2) LPS group; (3) EGCG group; and (4) LPS + EGCG group. The control group was injected with 0.9% saline + sodium carboxymethylcellulose. EGCG group and LPS + EGCG group were injected intraperitoneally with 200 mg/kg EGCG (Sigma-Aldrich, St. Louis, USA, Cat. NO. M5250). Five days later, to establish the animal model of sepsis-induced cardiac dysfunction, the LPS group and LPS + EGCG group were injected through tail vein with 15 mg/kg LPS (Sigma-Aldrich, St. Louis, USA, Cat. NO. L2880). The cardiac function was measured 24 h after LPS treatment. And then the myocardium was harvested for further examination.

Chen B., Li Y.F., Fang Z., Cai W.Y., Tian Z.Q., Li D, & Wang Z.M. (2024). Epigallocatechin-3-gallate protects sepsis‐induced myocardial dysfunction by inhibiting the nuclear factor-κB signaling pathway. Heliyon, 10(5), e27163.

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Synergistic Effects of Natural Compounds on Cancer Cell Proliferation

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LAPC-4-AI and PC-3 cells were seeded into opaque-wall 96-well plates at a density of 8×10³ per well. An inhibition curve was achieved for individual compound including EGCG, Q and Doc by incubation of both cell lines with multiple doses of each compound for 48h. A dose that leads to 10–30% cell growth inhibition by each compound was selected for the combination study. Cells were treated with the following: vehicle control (DMSO), 40μM EGCG (Sigma-Aldrich, St Louis, MO), 5μM Q (Sigma-Aldrich), 5nM Doc (Sigma-Aldrich), EGCG+Q, EGCG+Doc, Q+Doc, or EGCG+Q+Doc for 24 and 48h. In addition, the combined effect of the mixture was compared with a higher dose of Doc at 20nM. Cell proliferation was measured with adenosine triphosphate (ATP) assay using the CellTiter-Glo® Luminescent cell viability assay kit (Promega Corporation, Madison, WI). To minimize the effect of hydrogen peroxide (H₂O₂) that may be formed by autoxidation and/or dimerization of EGCG and Q in cell culture medium [28 (link)], 50 units/mL of catalase was added to the medium prior to EGCG, Q and Doc in all the experiments in the present study. There were four wells for each of the treatments and the experiment was repeated twice.

Wang P., Henning S.M., Heber D, & Vadgama J.V. (2015). Sensitization to docetaxel in prostate cancer cells by green tea and quercetin. The Journal of nutritional biochemistry, 26(4), 408-415.

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EGCG Ameliorates Diabetic Sexual Dysfunction in Rats

Cited 1 time

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One week after screening, the rats were tested for sexual behavior before STZ injection. Then, the rats were randomly divided into three groups: vehicle-treated control group (Control, n = 8), vehicle-treated diabetic group (DM, n = 8), and EGCG-treated diabetic group (DM + EGCG, n = 9).
Diabetes was induced by a single intraperitoneal injection of freshly prepared STZ (65 mg/kg; Sigma). Control animals were intraperitoneally injected with an equivalent volume of sodium citrate buffer. Fasting blood glucose levels were measured from the tail vein before and 72 h after STZ injection, using a glucometer (Contour Plus, Bayer, Germany). Rats exhibiting fasting glucose levels greater than 300 mg/dL after STZ injection were considered as diabetic rats [35 (link),37 (link)], and were used in this study.
All the subsequent treatments were done by gavage every day between 9:00 AM and 11:00 AM for 14 consecutive days. Animals in the EGCG-treated diabetic group were administrated at a dose of 40 mg/kg/day of EGCG (Sigma), starting one week after STZ injection. The groups of vehicle-treated control and vehicle-treated diabetes received equal volumes of distilled water. Fasting blood glucose levels were measured again on days 7 and day 14 of EGCG treatment. Body weight and sexual behavioral parameters were recorded before and on day 7 and day 14 of EGCG treatment.

Huang A.C., Yeh T.C., Wu N.C., Yeh C.Y., Lin P.H, & Yeh K.Y. (2022). Protective Effects of Epigallocatechin Gallate for Male Sexual Dysfunction in Streptozotocin-Induced Diabetic Rats. International Journal of Molecular Sciences, 23(17), 9759.

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Epigallocatechin gallate treatment in pregnant mice

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Epigallocatechin gallate treatment was performed as described previously.³⁷ Concentrations of either 1 or 10 μM Epigallocatechin gallate (Sigma) were given to wild-type nondiabetic and diabetic pregnant mice at embryonic day 5.5 in drinking water.

Zhong J., Xu C., Albert Reece E, & Yang P. (2016). The green tea polyphenol EGCG alleviates maternal diabetes–induced neural tube defects by inhibiting DNA hypermethylation. American journal of obstetrics and gynecology, 215(3), 368.e1-368.e10.

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Comprehensive Metabolite Analysis Protocol

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N,N-dimethylformamide, acetonitrile, acetic acid, and methanol (chromatographic grade, Shanghai National Pharmaceutical Group Reagent Co, Shanghai, China). Methanol, sodium chloride, sodium carbonate, folin-phenol, disodium hydrogen phosphate, potassium dihydrogen phosphate, ninhydrin, stannous chloride, aluminum trichloride, anthrone, anhydrous dextrose, concentrated sulfuric acid (analytical grade), Shanghai National Pharmaceutical Group Reagent Co, Shanghai China. Epi catechin (EC), epigallo catechin (EGC), catechin (D, L-C), epigallo catechin gallate (EGCG), epigallo catechin gallate (ECG), gallocatechin gallate (GCG), aspartic acid, serine, glutamic acid, glycine, histidine, arginine, threonine, alanine, proline, theanine, cysteine, tyrosine, valine, methionine, lysine, iso leucine, leucine, phenylalanine standard, n-alkane mixed standard (C7~C30), Sigma-Aldrich Corp., St. Louis, MO, USA. Ethyl decanoate (99%), Shanghai Aladdin Biochemical Technology Co, Shanghai, China.

Wang K., Xiao Y., Xie N., Xu H., Li S., Liu C., Huang J., Zhang S., Liu Z, & Yin X. (2023). Effect of Leaf Grade on Taste and Aroma of Shaken Hunan Black Tea. Foods, 13(1), 42.

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Epigallocatechin Gallate Treatment in Rats

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Rats in the experimental group were administered epigallocatechin gallate (Sigma-Aldrich Co., St. Louis, MO, USA) once before surgery and once daily after surgery at a dose of 1 mL of epigallocatechin gallate solution prepared as 100 mg/kg via gastric gavage. The rats in the control group were given equivalent volumes of saline via gavage. The doses and administration protocol were previously described by Meng et al. [13 (link)], Choi et al. [14 (link)], Unno and Takeo [15 (link)].

İğde M., Onur Öztürk M., Yaşar B., Hakan Bulam M., Ergani H.M, & Ünlü R.E. (2019). Antithrombotic effect of epigallocatechin gallate on the patency of arterial microvascular anastomoses. Archives of Plastic Surgery, 46(3), 214-220.

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Characterization of Antioxidant Compounds

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Methanol (analytical grade) was purchased from VWR Chemicals (Fontenay-sous-Bois, France). Methanol and sulfuric acid (HPLC grade), anhydrous sodium sulphate, isooctane, glacial acetic acid, trichloroacetic acid, orthophosphoric acid (all analytical grade), sodium carbonate, absolute ethanol, sodium hydroxide, chloroform, all were purchased from Merck (Darmstadt, Germany). 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,6-di-tert-butyl-4-methylphenol (BHT), 2-(1,1dimethylethyl)-1,4-benzenediol (TBHQ), (-)-epicatechin (purity ≥90%), (-)-epigallocatechin gallate (purity ≥80%), (-)-epigallocatechin gallate (purity ≥95%), 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox) (purity 97%), Tween ® 40, linoleic acid, βcarotene, Phenol reagent Folin & Ciocalteu`s, sodium nitrite, aluminium chloride, potassium iodide, soluble starch, p-anisidine, thiobarbituric acid, 2,4-DNPH, were all purchased from Sigma-Aldrich (Steinheim, Germany). n-hexane for spectroscopy was supplied from BDH Prolabo (Leuven, Belgium).

Martins C., Vilarinho F., Silva A.S., Andrade M., Machado A.V., Castilho M.C., Sá A., Cunha A., Vaz M.F, & Ramos F. (2018). Active polylactic acid film incorporated with green tea extract: Development, characterization and effectiveness. Industrial Crops & Products., 123.

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Hair Follicle Regulation by Estrogen Compounds

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Male 6-week-old B6 mice (3 mice per treatment) with hair follicles in telogen, as indicated by pink skin, were shaved [54 (link)]. Shortly after shaving 10 nmoles of the following compounds in 200 μl acetone was topically applied to the whole dorsal skin: 1) vehicle only control, 2) 17-β-estradiol (E2, Sigma, St. Louis, MO), 3) an anti-estrogen (ICI 182,780, Tocris, Minneapolis, MN) 4) an aromatase inhibitor epigallocatechin gallate (EGCG, Sigma-Aldrich, St. Louis, MO), or 5) E2 plus EGCG. For E2 plus EGCG 10 nmoles of E2 plus 10 nmoles of EGCG were combined in acetone and added simultaneously. Mice were euthanized 4, 12, or 24 hours later. Skin was fixed with Tellyzneski-Fekete’s acid-alcohol-formalin solution, placed in RNAlater RNA stabilization solution (Ambion, Austin, TX), or frozen in optimum cutting solution (OCT, Fisher scientific Waltham, MA).

Everts H.B., Silva K.A., Schmidt A.N., Opalenikx S., Duncan F.J., King LE J.r., Sundberg J.P, & Ong D.E. (2021). Estrogen regulates the expression of retinoic acid synthesis enzymes and binding proteins in mouse skin. Nutrition research (New York, N.Y.), 94, 10-24.

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Streptozotocin-Induced Diabetes with EGCG Treatment

Cited 2 times

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STZ (Sigma) was freshly dissolved in 0.1 M sodium citrate buffer (pH 4.5) at a dose of 65 mg/kg body weight. The STZ dose was chosen based on the previous studies [6 (link),35 (link)]. EGCG (Sigma) was dissolved in sterile distilled water and administered orally at a dose of 40 mg/kg/day of EGCG (Sigma) one week after STZ injection. The dose of EGCG was chosen based on a previous study [36 (link)].

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Oxidative Stress Modulation and Cisplatin Resistance

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Harvested adult worms were washed and incubated with 5 mM H₂O₂ for 30 min, followed by lysis and western blot analysis. For recovery from H₂O₂ exposure, washed worms were put onto seeded NGM plates for the indicated times. Epigallocatechin gallate (EGCG): EGCG (Sigma) containing plates were prepared by spreading 200 µL of 400 µM EGCG dissolved in water on unseeded NGM plates to obtain a final concentration of 5.7 µM. Spots of concentrated OP50 were applied for food after the plates had dried. L3 larvae were transferred onto NGM + EGCG plates for 24 h and transferred onto fresh EGCG plates for another 24 h. Worms were washed 3 times with M9 followed by lysate preparation or exposed to cisplatin and tested for survival. Cisplatin treatment: Cisplatin plates were prepared using MYOB media with 2% agar in which the drug was added at a final concentration of 300 μg/mL. Cisplatin solution (1 mg/mL, Accord Healthcare AB) was added to autoclaved medium after cooling to 52 °C. Young adults (24 h post L4 stage) were collected, washed and incubated on cisplatin plates for 3 or 6 h. Worms were harvested and processed for lysate preparation.

Raj D., Billing O., Podraza-Farhanieh A., Kraish B., Hemmingsson O., Kao G, & Naredi P. (2021). Alternative redox forms of ASNA-1 separate insulin signaling from tail-anchored protein targeting and cisplatin resistance in C. elegans. Scientific Reports, 11, 8678.

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