Enoxaparin (Sigma, St. Louis, MO, USA), 4 mg/kg, was given subcutaneously 30 min before MV, based on our previous in vivo study that showed 4 mg/kg Enoxaparin inhibited blood coagulation and lung injury without significant bleeding tendency [8 (link)].
Enoxaparin
Manufactured by Merck Group
Sourced in United States
Enoxaparin is a low-molecular-weight heparin (LMWH) used as an anticoagulant. It is a pharmaceutical product manufactured by Merck Group.
Automatically generated - may contain errors
4 protocols using enoxaparin
1
Enoxaparin Inhibits Lung Injury
2
Enoxaparin Suppresses Coagulation and Lung Injury
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Enoxaparin (Sigma, St. Louis, MO, USA), 4 mg/kg, was given subcutaneously 30 min before MV, based on our previous in vivo study that revealed 4 mg/kg Enoxaparin suppressed blood coagulation and lung injury without significant bleeding tendency [27 (link),38 (link)].
3
TgPDCD5 Heparin Binding Kinetics
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Quantitative estimations of heparin-binding
affinities for some proteins using ITC have been reported.49 (link)−51 (link) To investigate the interaction between TgPDCD5 and heparin sulfate
(H3393, Sigma-Aldrich) or Enoxaparin (E0180000, Sigma-Aldrich), ITC
was performed using a Nano ITC instrument (TA Instruments). Aliquots
of 4 μL of 3 mM TgPDCD5 were injected into 0.12 mM heparin sulfate
or Enoxaparin in 25 mM phosphate buffer at pH 4.5 with 100 mM NaCl
while maintaining a temperature of 37 °C with 250 rpm stirring.
Background heat from the protein to buffer titrations was subtracted.
The thermal parameters (enthalpy ΔH and entropy
ΔS), stoichiometry of the binding (n), and dissociation constant (Kd) were derived by fitting the data to an independent binding model
using Launch NanoAnalyze v2.3.6 software.
affinities for some proteins using ITC have been reported.49 (link)−51 (link) To investigate the interaction between TgPDCD5 and heparin sulfate
(H3393, Sigma-Aldrich) or Enoxaparin (E0180000, Sigma-Aldrich), ITC
was performed using a Nano ITC instrument (TA Instruments). Aliquots
of 4 μL of 3 mM TgPDCD5 were injected into 0.12 mM heparin sulfate
or Enoxaparin in 25 mM phosphate buffer at pH 4.5 with 100 mM NaCl
while maintaining a temperature of 37 °C with 250 rpm stirring.
Background heat from the protein to buffer titrations was subtracted.
The thermal parameters (enthalpy ΔH and entropy
ΔS), stoichiometry of the binding (n), and dissociation constant (Kd) were derived by fitting the data to an independent binding model
using Launch NanoAnalyze v2.3.6 software.
4
Heparin-based Affinity Chromatography
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Heparin-Sepharose 6 Fast Flow Affinity Chromatography Media and nitrocellulose blotting membrane (0.2 μm) were obtained from GE Healthcare (Velizy-Villacoublay, France). Streptavidin Alexa Fluor 680 conjugate was purchased from Molecular Probes (Fisher Scientific, Saint-Aubin France). Enoxaparin, heparin from porcine intestinal mucosa and bovine serum albumin were from Sigma-Aldrich (Sigma-Aldrich, Saint Quentin Fallavier, France). All other chemicals were of analytical grade.
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