2 2 azinobis 3 ethylbenzothiazoline 6 sulfonic acid

Methanol, hexane, chloroform, ethyl acetate and ethanol were purchased from Junsei Chemical Co., Ltd., Tokyo, Japan. Potassium phosphate monobasic and dibasic, xanthine, xanthine oxidase, allopurinol, and hydrochloric acid were obtained from Sigma-Aldrich Corp., St. Louis, MO, USA. Reagents including 1,1-diphenyl-2-picrylhydrazyl (DPPH), sodium acetate, acetic acid, 2,2′-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), potassium peroxodisulfate, and dibutyl hydroxytoluene (BHT) were supplied by Kanto Chemical Co. Inc., Tokyo, Japan. Four bacteria including Staphylococcus aureus, Escherichia coli, Bacillus subtilis, and Proteus mirabilis were provided by Sigma-Aldrich Corp., St. Louis, MO USA. All chemicals used were of analytical grade.

Extraction solvents including methanol, hexane, ethyl acetate, and butanol were purchased from Junsei Chemical Co., Ltd., Tokyo, Japan. Standards including gallic acid, rutin, butylated hydroxytoluene (BHT), kojic acid were purchased from Kanto Chemical Co., Inc., Tokyo, Japan. Palmitic acid was obtained from FUJIFILM Wako Pure Chemical Corporation.
Chemicals including sodium hypochlorite (NaClO), Folin-Ciocalteu’s reagent, sodium carbonate (Na₂CO₃), aluminum chloride (AlCl₃), 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), potassium persulfate (K₂S₂O₈), 2,2-diphenyl-1-picrylhydrazyl (DPPH), sodium acetate (CH₃COONa), β-carotene, chloroform, linoleic acid, tween 40, dipotassium phosphate (K₂HPO₄), monopotassium phosphate (KH₂PO₄), potassium ferricyanide (K₃[Fe(CN)₆]), trichloro acetic acid (CCl₃COOH), ferric chloride (FeCl₃), dimethyl sulfoxide (DMSO), monosodium phosphate (NaH₂PO₄), sodium chloride (NaCl), hydrochloric acid (HCl) were obtained from Kanto Chemical Co., Inc., Tokyo, Japan. While tyrosinase, L-tyrosine, α-amylase, starch, iodine, 3-(4,5-dimethylthiazolyl)2,5-diphenyl-tetrazolium bromide (MTT), Iscove’s Modified Dulbecco’s Medium (IMDM), and cell lysis buffer were purchased from Sigma-Aldrich, St. Louis, MO, USA.
K562 and Meg-01 cell lines were obtained from American Type Culture Collection (ATCC), Virginia, United States.