One gram of the leaves’ powder was placed into a 20 mL head-space vial. The vials were sealed using crimp-top caps with TFE-silicone headspace septa (Agilent). Each vial was placed at 60°C for 10 min, and then a 65 μm divinylbenzene/carboxen/polydimethylsiloxane fiber (Supelco, Bellefonte, PA, United States) was exposed to the headspace of the sample for 20 min at 60°C. The volatile compound identification and quantification were carried out using an Agilent Model 7890 BGC and a 7000D mass spectrometer (Agilent), equipped with a 30 m × 0.25 mm × 1.0 μm DB-5 ms capillary column. Helium was used as the carrier gas at a flow rate of 1.0 ml/min. The injector temperature and detector temperature were 250°C and 280°C, respectively. The oven temperature was programmed at 40°C for 5 min, increased by 6°C/min to 280°C, and held for 5 min. The ionization voltage was 70 eV. Mass spectra were scanned from 30 to 350 amu. The compounds were identified by comparing the mass spectra with the MWGC database (Metware Biotechnology Co., Ltd., Wuhan, China) and a linear retention index.
Model 7890b gc
Manufactured by Agilent Technologies
Sourced in Switzerland
The Agilent 7890B Gas Chromatograph (GC) is a laboratory instrument designed for the separation, identification, and quantification of chemical compounds in a sample. It features advanced electronics, intuitive software, and high-performance components to deliver reliable and repeatable results.
Automatically generated - may contain errors
Lab products found in correlation
7000d mass spectrometer, by Agilent Technologies (2 mentions)
Db 5ms capillary column, by Agilent Technologies (2 mentions)
Tfe silicone headspace septa, by Agilent Technologies (1 mentions)
Divinylbenzene carboxen polydimethylsiloxane fiber, by Merck Group (1 mentions)
Model 340, by Mettler Toledo (1 mentions)
Agilent hp innowax column, by Agilent Technologies (1 mentions)
3 protocols using model 7890b gc
1
Volatile Compound Identification in Leaves
2
Volatile Compound Analysis by GC-MS
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Volatile emission collections were carried out with the SPME method previously described (11 (link)). The identification and quantification of VOCs were conducted by a Model 7890B GC and a 7000D mass spectrometer (Agilent), equipped with a DB-5MS capillary column (5% phenyl-polymethylsiloxane, 30 m x 0.25 mm x 1.0 μm). The GC-MS data were unit variance scaled, and then, principal component analysis (PCA) was conducted. The hierarchical cluster analysis (HCA) results were presented as heatmaps. Pearson correlation coefficients (PCCs) were calculated by R. Differentially accumulated metabolites (DAMs) were characterized with the criterions of VIP ≥1 and absolute Log2-fold change ≥1. The VIP values were obtained from OPLS-DA (Orthogonal Partial Least Squares Discrimination Analysis) results. The metabolites identified with NIST were further annotated by KEGG compound database and further mapped to KEGG pathway. Metabolite sets enrichment analysis (MSEA) was applied to analysis pathways containing DAMs, using hypergeometric test's p-values to determine the significance.
3
Fermented Jujube Juice Characteristics
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All the samples were centrifuged (7783×g for 10 min) for the analysis of fermented jujube juice characteristics (Settanni et al., 2012) . Soluble solid was analyzed using a refractometer according to AOAC guidelines. The pH was measured using a pH meter (Model 340, Mettler Toledo Gmb H, Schwerzenbach, Switzerland) and the titratable acidity (expressed as g kg -1 tartaric acid) was determined using the AOAC Official Method 962.12.
Ethanol contents were determined using an Agilent Model 7890B GC with reference to the national standard GB 5009. 225-2016 225- (SAC, 2016) ) published by the Standardization Administration of China. Briefly, the fermentation broth was filtered by organic membrane (0.45μm, PTFE) and then put into gas phase vial (2 mL, 9-425 transparent thread chromatographic injection vial). Chromatographic conditions are as follows: Agilent HP-INNOWax column (60 m × 0.25 mm × 0.25 μm, Agilent J&W, USA); column temperature, 50°C; temperature of gasifier and detector, 240°C, carrier gas (high purity nitrogen) flow rate, 30 mL/min; H2 flow rate, 30 mL/min; air flow rate, 300 mL/min. Ethanol were detected using a FID detector.
Ethanol contents were determined using an Agilent Model 7890B GC with reference to the national standard GB 5009. 225-2016 225- (SAC, 2016) ) published by the Standardization Administration of China. Briefly, the fermentation broth was filtered by organic membrane (0.45μm, PTFE) and then put into gas phase vial (2 mL, 9-425 transparent thread chromatographic injection vial). Chromatographic conditions are as follows: Agilent HP-INNOWax column (60 m × 0.25 mm × 0.25 μm, Agilent J&W, USA); column temperature, 50°C; temperature of gasifier and detector, 240°C, carrier gas (high purity nitrogen) flow rate, 30 mL/min; H2 flow rate, 30 mL/min; air flow rate, 300 mL/min. Ethanol were detected using a FID detector.
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