In this study, the manual SPME was used for collecting the volatile pyrazines. The method of GC-MS was established for the separation and identification of different kinds of pyrazines. For the SPME, the vials (20 mL) were filled with 1 mL solutions composed of a mixture of pyrazine solution (1 × 10−5 mol L−1) and BSA solution (0, 5, 15 × 10−4 mol L−1, respectively). The vials were heated at 323 K under stirring. The extraction fiber of SPME coatings with 50/30 μm DVB/CAR/PDMS was inserted into the vials and extracting the head space compounds for 30 minutes. When the extraction was completed, the fiber was placed in the injection port of the GC-MS system (Agilent 7890B-5977A, Agilent, U.S.A) with GC column (HP-5MS, 30 m × 250 μm ID). The time of desorption and the injection temperature were 5 minutes and 523 K, respectively. The oven heat program began at 308 K (3 minutes) and then was increased to 553 K at 283 K min−1, and maintained at 553 K for 5 min. The MS with electronic impact ionization source (ionization voltage: 70 eV) operated in scan mode and the mass detection range was from 40 to 400 m/z. In addition, the ability of pyrazine to release from BSA is reflected by the peak area ratios (area of pyrazine with BSA/area of pyrazine without BSA).
Agilent 7890b 5977a
Manufactured by Agilent Technologies
Sourced in United States
The Agilent 7890B-5977A is a gas chromatography-mass spectrometry (GC-MS) system designed for analytical laboratory applications. It combines a gas chromatograph (7890B) and a mass spectrometer (5977A) to provide separation, identification, and quantification of complex chemical mixtures. The system is capable of analyzing a wide range of volatile and semi-volatile organic compounds.
Automatically generated - may contain errors
Lab products found in correlation
Agilent 1100, by Agilent Technologies (3 mentions)
Hp 5ms, by Agilent Technologies (1 mentions)
N 1000, by Eyela (1 mentions)
Bstfa tmcs, by Merck Group (1 mentions)
Crm47885, by Merck Group (1 mentions)
Silent crusher m, by Heidolph (1 mentions)
Fused silica 24 ga, by Merck Group (1 mentions)
Oasis hlb prime cartridges, by Waters Corporation (1 mentions)
Masshunter software, by Agilent Technologies (1 mentions)
Db 5ms capillary column, by Agilent Technologies (1 mentions)
Db wax, by Agilent Technologies (1 mentions)
16 protocols using agilent 7890b 5977a
1
Pyrazine Extraction and Identification
2
Gas Chromatography-Mass Spectrometry Analysis of Essential Oils
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The essential oils were analyzed using an Agilent 7890B/5977A gas chromatograph (Agilent Technologies) equipped with a 60 m × 0.25 mm × 0.25 μm DB-WAX column. The mass-selective detector operated in scan range from m/z 30 to m/z 550 at 70 eV. Initially, the oven temperature was 40 °C, and then raised in stepwise manner to 50 °C, 92 °C, 120 °C and 230 °C at a rate of 5 °C/min, 6 °C/min, 3 °C/min and 5 °C/min, respectively. The carrier gas was helium, and the flow rate was 1.2 mL/min. The sample diluted with dichloromethane was injected into the gas chromatograph with a split ratio of 33.3. By comparing with the National Institute of Standards and Technology (NIST) MS spectral database (version 2017), the sample constituents were identified [13 (link)].
3
Guaiacol Hydrodeoxygenation Using Ni2P@HZSM-5 Catalyst
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The HDO
reactions were performed in a 100 mL high-pressure reactor (Shanghai
Yanzheng, China). The activity of Ni2P@hierarchical HZSM-5-X(Y)
was evaluated by using HDO of guaiacol. The high-pressure reactor
was checked for leaks before each reaction. Guaiacol, dodecane, and
catalyst were added to the reactor. The air was replaced by purging
five times with 3 MPa H2. Then, the reactor was heated
to 300 °C at a stirring speed of 800 rpm. After each reaction,
the liquid products were collected while directly removing the gaseous
products. The liquid products were analyzed, respectively, by GC (GC1120,
China) and GC–MS (Agilent 7890B-5977A, USA). The conversion
and the yield were computed by the following definition: where i represents products
cyclohexane, methylcyclopentane,
methylcyclohexane, anisole, and phenol in the reaction; nGua0 and nGua represent the content of guaiacol before
and after the reaction, respectively; ni is the content of product i; and t is the reaction
time.
reactions were performed in a 100 mL high-pressure reactor (Shanghai
Yanzheng, China). The activity of Ni2P@hierarchical HZSM-5-X(Y)
was evaluated by using HDO of guaiacol. The high-pressure reactor
was checked for leaks before each reaction. Guaiacol, dodecane, and
catalyst were added to the reactor. The air was replaced by purging
five times with 3 MPa H2. Then, the reactor was heated
to 300 °C at a stirring speed of 800 rpm. After each reaction,
the liquid products were collected while directly removing the gaseous
products. The liquid products were analyzed, respectively, by GC (GC1120,
China) and GC–MS (Agilent 7890B-5977A, USA). The conversion
and the yield were computed by the following definition: where i represents products
cyclohexane, methylcyclopentane,
methylcyclohexane, anisole, and phenol in the reaction; nGua0 and nGua represent the content of guaiacol before
and after the reaction, respectively; ni is the content of product i; and t is the reaction
time.
4
Methionine Supplementation in Fish Meal-Soy Protein Diet
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The basic diet (110 g/kg fish meal and 400 g/kg soy protein concentrate) was based on our previous data [15 (link),16 (link)]. Different levels of methionine (0, 2, 4, 6, 8, or 10 g/kg) were supplemented in the basic diet based on the rule of equal nitrogen and our previous studies [14 (link),16 (link)], showed in Table 6 , Table 7 and Table 8 .
Proximate analysis (moisture, crude protein, crude lipid, ash, and gross energy) of experimental feed and M. albus was performed based on our previous papers [53 (link)]. Amino acids were analyzed by an automatic amino acid analyzer (Agilent-1100, Agilent Technologies Co., Ltd., Santa Clara, CA, USA) based on Wijerath’s method [54 (link)], and fatty acids were analyzed by GC-MS (Agilent 7890B-5977A, Agilent Technologies Co., Ltd., Santa Clara, CA, USA) based on Jin’s method [55 (link)], the results are shown inTable 2 and Table 3 .
Proximate analysis (moisture, crude protein, crude lipid, ash, and gross energy) of experimental feed and M. albus was performed based on our previous papers [53 (link)]. Amino acids were analyzed by an automatic amino acid analyzer (Agilent-1100, Agilent Technologies Co., Ltd., Santa Clara, CA, USA) based on Wijerath’s method [54 (link)], and fatty acids were analyzed by GC-MS (Agilent 7890B-5977A, Agilent Technologies Co., Ltd., Santa Clara, CA, USA) based on Jin’s method [55 (link)], the results are shown in
5
Identification of Lipid Compounds by GC-MS
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A standard mixture containing 39 FAMEs (GLC 566, Nu Chek Prep Inc., MA, United States) or standardized AcOMEs obtained in the previous section (Study II) were analyzed by GC–MS chromatography (Agilent 7890B/5977A, Agilent Technologies Inc., CA, United States) to identify the lipids. A previously developed method was used (Castillo et al., 2020 (link)). Having as reference fragmentation patterns of FAMEs (Jabbar et al., 2014 ; Busta et al., 2016 (link)) and Mclafferty rearrangement characteristic of esters (McLafferty, 1956 (link); Weiler, 1972 (link)), the expected fragmentation patterns of each of the free AcO-acetogenins reported here (based on their structure) were matched to their observed mass spectra (Supplementary Figure 2 ).
6
Quantification of Bacterial Polyhydroxybutyrate
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The crude PHB was diluted with chloroform at 1:40 (dry weight of SS:volume of chloroform, g:mL) at 40°C for 11 h, and the pellet was collected by centrifugation, dried at 70°C for 8 h in the oven. Then, the PHB extract was diluted with chloroform at 80°C using a rotary evaporator (Eyela N-1000), and dried to a constant mass. A thin film of pure PHB was obtained and quantified using spectrometric analysis based on the standard curve (Law & Slepecky, 1961 (link)). The purity of PHB was analyzed by gas chromatography (GC–MS, Agilent 7890B/5977A) (Albuquerque et al., 2010 (link); Alsafadi & Al-Mashaqbeh, 2017 (link)).
7
Methionine Supplementation in Fish Diets
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A basic diet (110 g/kg fish meal; 400 g/kg soy protein concentrate), as prepared in previous studies (Zhang et al., 2019 (link); Hu et al., 2021b (link)), was supplemented with different levels of methionine (0, 2, 4, 6, 8, or 10 g/kg). Table 1 shows the composition and nutrition of these methionine diets.
The proximate analysis (moisture, crude lipid, crude protein, ash, and gross energy) of the experimental feed treatments was determined based on our previous paper (Hu et al., 2021c (link)). Amino acids (Table 2 ) were analyzed by an automatic amino acid analyzer (Agilent-1,100, Agilent Technologies Co., Ltd., Santa Clara, CA, United States), by referring to the methodology of Wijerath Wiriduge et al. (2020) (link), while fatty acids (Table 3 ) were analyzed by GC–MS (Agilent 7890B-5977A, Agilent Technologies Co., Ltd., Santa Clara, CA, United States) according to Jin et al. (2017) (link).
The proximate analysis (moisture, crude lipid, crude protein, ash, and gross energy) of the experimental feed treatments was determined based on our previous paper (Hu et al., 2021c (link)). Amino acids (
8
Propamocarb Residue Analysis Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A propamocarb analytical standard was provided by Sigma-Aldrich® (CAS: 24579-73-5) (Merck, United States). Ten grams of homogenized sample were added to a 50 mL colorimetric tube and further homogenized for 5 min. Then, 20 mL acetonitrile and 2.5 g NaCl were added to the sample in the 50 mL colorimetric tube, and mixed for 5 min with shaking at 500 g/min using a mechanical shaker. Samples were allowed to settle for 30 min and then vortex mixed and evaporated until dryness; this step was repeated several times and the resulting material was collected. The sample was then filtered through a polypropylene filter (Merck, United States) (0.22 μm) until no residual particles remained. The levels of propamocarb residue were then analyzed using the Agilent 7890B-5977A gas chromatography system (Agilent Technologies) with a 30 m × 0.25 mm × 0.25 μm capillary column (HP-5MS). The oven temperature was programmed at 40°C 2 min, increased to 200°C at 25°C/min and held for 8 min, sample inlet: 240°C, non-split injection, flow rate of 1.0 mL min–1.
9
Methionine Supplementation in High-Fat Fish Diet
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The basic feed (60 g/kg fat) was made according to our previous paper [32 (link), 33 (link)], named Con; and the high-fat basic feed (120 g/kg fat) was based on our study on M. Albus [36 (link)], different levels of methionine (0, 4 g/kg, 8 g/kg, and 12 g/kg) were supplemented into high-fat diet obeyed the principle of equal nitrogen referenced our study [32 (link), 33 (link)], named as, HFD+M0, HFD+M4, HFD+M8, and HFD+M12, respectively. Experimental diets and levels of nutrition is shown in Table 1 .
The method of proximate analysis (moisture, crude protein, crude lipid, and ash) of experimental feed was referenced in our paper [37 (link)]. Amino acids were determined by an automatic amino acid analyzer (Agilent-1100, Agilent Technologies Co., Ltd., Santa Clara, CA, USA) referenced by the method reported by Wiriduge et al. [38 (link)], fatty acids were analyzed by GC-MS (Agilent 7890B-5977A, Agilent Technologies Co., Ltd., Santa Clara, CA, USA) referenced the method reported by Jin et al. [39 (link)], as shown in Tables2 and 3 .
The method of proximate analysis (moisture, crude protein, crude lipid, and ash) of experimental feed was referenced in our paper [37 (link)]. Amino acids were determined by an automatic amino acid analyzer (Agilent-1100, Agilent Technologies Co., Ltd., Santa Clara, CA, USA) referenced by the method reported by Wiriduge et al. [38 (link)], fatty acids were analyzed by GC-MS (Agilent 7890B-5977A, Agilent Technologies Co., Ltd., Santa Clara, CA, USA) referenced the method reported by Jin et al. [39 (link)], as shown in Tables
10
Comprehensive Carbohydrate and Protein Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The total sugar content was determined by the phenol–sulphuric acid colorimetric method and glucose was used as reference standard for polysaccharide determination. Protein content was determined using Pierce BCA protein quantification kit with bovine serum albumin (BSA) as standard according to manufacturer's instruction. Monosaccharide composition was analyzed by Agilent 7890B/5977A gas chromatography-mass spectrometry (GC-MS) analysis using the TR-5MS chromatography column (60 m × 0.25 mm × 0.25 μm) from Thermo Fisher Scientific, Inc. (Grand Island, NY, USA). Briefly, a total of 2 mg of samples were hydrolyzed with 2 M trifluoroacetic acid (TFA) (3 mL) at 121°C for 2 h, and methanol was used to remove TFA completely. NaBH4 and water were added to reduce the residue for 5–8 h. After the samples were dried at 105°C for 10 min, it was acetylated by acetic anhydride at 105°C for 1 h. Finally, the monosaccharide compositions of RSGLP and BSGLP were measured by GC-MS method as described before (29 (link)).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!