Using a CCK8 test, the impact of catalpol on the viability of LPS-treated astrocytes was determined. Briefly, the cultures enriched astroglia were planted at a cell concentration of 4 ×104 cells/well in 96-well plates. The cells were then treated for 6 h with varying doses of catalpol (0, 125, 250, 500, and 1000 µM) with or without LPS (1 μg/ml). After the incubation period completion, 10 µL of CCK8 testing reagent (Dojindo, Japan) was applied to every well, followed by an extra 1 h of incubation at 37 °C. At 450 nm, the absorbance (OD) was calculated utilizing a Multiskan GO spectrophotometer (United States).
Multiskan go spectrophotometer
Manufactured by MultiSciences Biotech
Sourced in United States
The Multiskan GO spectrophotometer is a high-performance instrument designed for absorbance measurements in life science and analytical applications. It provides accurate and reliable data across a wide range of wavelengths, enabling users to conduct various types of spectroscopic analyses.
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Lab products found in correlation
4 protocols using multiskan go spectrophotometer
1
Catalpol's Impact on Astrocyte Viability
2
Quantifying Photosynthetic Pigments
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The analysis of chlorophyll a, chlorophyll b, and carotenoid contents was performed using the Multiskan GO spectrophotometer, as described previously [59 (link)]. The level of pigments was calculated according to equations reported previously [60 (link)].
3
Measuring Inflammatory Cytokine Levels
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HMC3 cells were plated into 6-well (2 × 105/well) dishes, grown for 20 h and treated with the tested compounds (1–10 μM) for 8 h followed by IFN-γ (100 ng/ml) stimulation. The release of NO in cell culture medium was evaluated by Griess assay according to manufacturer’s protocol (Thermo Fisher Scientific). In addition, the levels of IL-1β, TNF-α and IL-6 in medium pre-treated with 10 μM compound were determined using Human Instant ELISATM Kit following the manufacturer’s protocol (Invitrogen). The optical density at 450 nm was detected using Multiskan GO spectrophotometer.
4
Cytotoxicity Evaluation of Nanoparticles
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An MTT assay was conducted following an established protocol [30 (link),31 (link)] to assess the short-term cytotoxic effect of PPNPs on RL14 cardiomyocytes and NiH3T3 fibroblasts. It was employed as an indicator of the cells’ metabolic competence [32 (link)]. In summary, RL14 and NiH3T3 cells (4 × 103 cells/well, 96-well microplates) were incubated with 10 μL of 20 freshly prepared serial dilutions (1:2) of NSC, PAPESE, and PPNPs, ranging from 2000 to 3.81 ×10−3 µg/mL, for 24 h. At the end of the incubation, 10 μL MTT solution (5 mg/mL) was added to each well. The microplates were then incubated under constant stirring in the dark for 6 h at 37 °C. Then, 100 μL cold dimethylsulfoxide (DMSO) was added to each well to dissolve the formazan crystals, followed by gentle stirring in a gyratory shaker for between 12 to 24 h. After that, the optical density (OD) was measured with a Multiskan-go spectrophotometer employing a wavelength of 570 nm. Five independent experiments were performed in triplicate to verify the repeatability. Positive, DMSO 100%, and negative complete culture media without cell controls were implemented.
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