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Milliplex map mmp magnetic bead panels

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MILLIPLEX MAP MMP Magnetic Bead Panels are a multiplex assay system designed for the quantitative measurement of multiple analytes in a single sample. The panels utilize Luminex xMAP technology with magnetic beads to enable simultaneous detection and quantification of various matrix metalloproteinases (MMPs) or tissue inhibitors of metalloproteinases (TIMPs) in biological samples.

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Lab products found in correlation

Ab126780, by Abcam (2 mentions) Cxcl5, by R&D Systems (1 mentions) Image lab software, by Bio-Rad (1 mentions) Bio plex 200 system, by Bio-Rad (1 mentions)

Close spelling variants of this product

Milliplex panels MILLIPLEX MAP Magnetic beads

3 protocols using milliplex map mmp magnetic bead panels

1

Comprehensive Enzymatic Activity Profiling

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MMP and cathepsin gene expression was performed by quantitative PCR (qPCR) using validated Taqman probes (Life Technologies/Applied Biosystems, Carlsbad, CA). All qPCR results are represented as relative quantification (RQ) compared to the mock treated animals and corrected to actin levels. Several MMP levels were measured in BALF and cell culture supernatants using a bead assay (MILLIPLEX MAP MMP Magnetic Bead Panels, EMD Millipore, Billerica, MA) with the BioRad Bio-Plex 200 system (BioRad, Hercules, CA). Cathepsin tissue levels were determined by immunoblots and BALF cathepsin S and B activity assays as previously described46 (link). Cathepsin G BALF activity was determined by a commercial available colorimetric assay (Abcam; ab126780). BALF collagenase activity was determined by a colorimetric ninhydrin method, as previously described22 (link). Proteinase activity was also assessed with the same colorimetric ninhydrin method but utilizing casein as the substrate. BALF elastase activity was measured by the hydrolysis of N-succinyl-L-Ala-L-Ala-L-Ala-p-nitroanilide(Suc Ala3NA) per minute at 25°C and pH 8.0. BALF gelatinase activity was determined by gelatin zymography.
Foronjy R.F., Taggart C.C., Dabo A.J., Weldon S., Cummins N, & Geraghty P. (2014). Type I interferons induce lung protease responses following respiratory syncytial virus infection via RIG-I-like receptors. Mucosal immunology, 8(1), 161-175.
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2

Comprehensive Enzymatic Activity Profiling

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
MMP and cathepsin gene expression was performed by quantitative PCR (qPCR) using validated Taqman probes (Life Technologies/Applied Biosystems, Carlsbad, CA). All qPCR results are represented as relative quantification (RQ) compared to the mock treated animals and corrected to actin levels. Several MMP levels were measured in BALF and cell culture supernatants using a bead assay (MILLIPLEX MAP MMP Magnetic Bead Panels, EMD Millipore, Billerica, MA) with the BioRad Bio-Plex 200 system (BioRad, Hercules, CA). Cathepsin tissue levels were determined by immunoblots and BALF cathepsin S and B activity assays as previously described46 (link). Cathepsin G BALF activity was determined by a commercial available colorimetric assay (Abcam; ab126780). BALF collagenase activity was determined by a colorimetric ninhydrin method, as previously described22 (link). Proteinase activity was also assessed with the same colorimetric ninhydrin method but utilizing casein as the substrate. BALF elastase activity was measured by the hydrolysis of N-succinyl-L-Ala-L-Ala-L-Ala-p-nitroanilide(Suc Ala3NA) per minute at 25°C and pH 8.0. BALF gelatinase activity was determined by gelatin zymography.
Foronjy R.F., Taggart C.C., Dabo A.J., Weldon S., Cummins N, & Geraghty P. (2014). Type I interferons induce lung protease responses following respiratory syncytial virus infection via RIG-I-like receptors. Mucosal immunology, 8(1), 161-175.
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3

Multiplex Biomarker Profiling in BALF

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MMP9 (EMD MILLIPLEX MAP MMP Magnetic Bead Panels, EMD Millipore, Billerica, MA) and cytokines (IL-13, RANTES, CXCL2, TNF-α, MCP-1, IL-1β, IL-17, IL-10, CXCL1, G-CSF, IL-6 and human IL-8; Bio-Rad Cytokines Magnetic Bead Panels) were measured in BALF and cell culture supernatants using a bead assay (MILLIPLEX MAP MMP Magnetic Bead Panels, EMD Millipore, Billerica, MA) with the Bio-Rad Bio-Plex 200 system (Bio-Rad, Hercules, CA) or with commercially available ELISAs (CXCL5 and SCF; R&D Systems). BALF gelatinase activity was determined by gelatin zymography [13 (link)]. Densitometry was performed on MMP9 positive bands and represented densitometry units (DU) measured by pixel intensity of bands, using Bio-Rad Laboratories Image Lab software (version 4.0, build 16).
Dabo A.J., Cummins N., Eden E, & Geraghty P. (2015). Matrix Metalloproteinase 9 Exerts Antiviral Activity against Respiratory Syncytial Virus. PLoS ONE, 10(8), e0135970.
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