Matrigel plug

Manufactured by Corning

Sourced in United States

Matrigel plug is a gelatinous protein mixture secreted by Engelbreth-Holm-Swarm (EHS) mouse sarcoma cells. It is used as a basement membrane extract for cell culture applications.

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Lab products found in correlation

Matrigel, by Corning (2 mentions) Nod cb17 prkdcscid ncrcrl, by Charles River Laboratories (1 mentions) Drabkin s solution, by Merck Group (1 mentions) Recombinant mouse opn, by R&D Systems (1 mentions) Matrigel basement membrane matrix, by Corning (1 mentions) Von kossa, by Merck Group (1 mentions)

6 protocols using matrigel plug

Xenograft Tumor Growth Assay in NOD SCID Mice

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Female 7-9 week old NOD SCID (NOD.CB17-Prkdcscid/NCrCrl) mice were obtained from Charles River Laboratories. NOD/SCID mice (n=4 per group) were subcutaneously inoculated with ALDH1+ve and ALDH1-ve H460 CisR cell fractions at a density of 1×10³ cells/mouse within a Matrigel plug (Corning, USA). Tumor volumes were recorded thrice weekly using digital callipers. Tumor volumes were calculated using the modified ellipsoid formula [1/2 (Length x Width²)] [89 (link)]. Experimental endpoints were defined as a tumor volume of 500mm³ or 90 days post-inoculation, at which point animals were sacrificed and tumors harvested. All animal experiments were approved by the Ethics Board of Trinity College Dublin and carried out under a licence granted by the Health Products Regulatory Authority.

MacDonagh L., Gallagher M.F., Ffrench B., Gasch C., Breen E., Gray S.G., Nicholson S., Leonard N., Ryan R., Young V., O'Leary J.J., Cuffe S., Finn S.P., O'Byrne K.J, & Barr M.P. (2017). Targeting the cancer stem cell marker, aldehyde dehydrogenase 1, to circumvent cisplatin resistance in NSCLC. Oncotarget, 8(42), 72544-72563.

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In vivo Angiogenesis Assay using Matrigel Plug

Cited 1 time

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For the in vivo angiogenesis assay (Matrigel plug assay), 2X10⁵ MDA-MB-231 cells were mixed with 0.5 ml of growth factor-reduced Matrigel (Corning, Tewksbury, MA) and implanted subcutaneously into the flanks of nude mice. The following day, six mice in each group were treated with 100 µg/kg HDAC9 siRNAs or control oligonucleotides via daily subcutaneous injections between the two plugs on the back of the mice. For selective HDAC IIa inhibitor, TMP269, mice were administered with TMP269 by subcutaneous injections every other day at 15 mg/kg mice body weight between the two plugs on the back of the mice. The animals were sacrificed and the Matrigel plugs were excised 10 days after Matrigel injection. The excised plugs were homogenized and subjected to measure hemoglobin content with 100 µL of Drabkin’s solution (Sigma, St. Louis, MO) following manufactural instruction and our previous description [41 (link)].

Salgado E., Bian X., Feng A., Shim H, & Liang Z. (2018). Overexpression of HDAC9 Promotes Invasion and Angiogenesis of Triple Negative Breast Cancer by regulating microRNA-206. Biochemical and biophysical research communications, 503(2), 1087-1091.

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Kidney Capsule Transplantation of Stem Cells

Cited 2 times

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Kidney capsule transplantation models were performed as previously described^{17 (link),18 (link)}. 2-month-old male mice were anesthetized with isoflurane and shaved on the right flank and lower back prior to surgical site sterilization. A 5-mm incision was made below the rib arch to expose the right kidney. And a 2-mm pocket was opened in the renal capsule to implant a 5–8uL Matrigel plug (Corning, USA) containing 2000 to 3000 SSCs. After implantation, the renal capsule was then sealed with an electrocautery. The kidney was then relocated back to the peritoneal cavity prior to layered Vicryl suture (Johnson & Johnson, USA) closure of the peritoneal and skin incisions. Recipients were euthanized by CO₂ narcosis 4 weeks after transplantation. Kidneys were carefully dissected, fixed with 4% paraformaldehyde (PFA) for 6 hours and mineralized organoids were assessed by μCT analysis.

Xu R., Li N., Shi B., Li Z., Han J., Sun J., Yallowitz A., Bok S., Xiao S., Wu Z., Chen Y., Xu Y., Qin T., Lin Z., Zheng H., Shen R, & Greenblatt M. (2023). Schnurri-3 inhibition rescues skeletal fragility and vascular skeletal stem cell niche pathology in a mouse model of osteogenesis imperfecta. Research Square.

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Ectopic Bone Formation in Nude Mice

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Six to eight-week-old male KSN/Slc Nude mice were anaesthetized. The kidney was externalized through a 1-cm incision and a 2-mm pocket was made in the renal capsule. A 5-µl Matrigel plug (Corning, 356231) containing 10,000 cells was implanted underneath the capsule and kidney was replaced back into the body cavity. Animals were euthanized after six weeks. After death, kidneys were fixed overnight with 4% PFA and bone formation was detected by μCT. Samples were subjected to infiltration, embedding and sectioning. Von Kossa staining was performed to detect the mineralized bones.

Tsukasaki M., Komatsu N., Negishi-Koga T., Huynh N.C., Muro R., Ando Y., Seki Y., Terashima A., Pluemsakunthai W., Nitta T., Nakamura T., Nakashima T., Ohba S., Akiyama H., Okamoto K., Baron R, & Takayanagi H. (2022). Periosteal stem cells control growth plate stem cells during postnatal skeletal growth. Nature Communications, 13, 4166.

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OPN-enriched Matrigel Plug Assay

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OPN-enriched in vivo Matrigel plug assay was performed according to the manufacturer’s instructions (Matrigel Basement Membrane Matrix, catalog 354234, Corning) and previous report (47 (link)). A total of 6 male 5-month-old WT mice were used. In brief, 500 μL Matrigel (catalog 354234, Corning) was mixed with 100 μL of 50 μg mouse recombinant OPN (R&D Systems) or PBS (as vehicle). Mixed solution was subcutaneously injected to the groin of anesthetized mice. After 14 days, the Matrigel plug was removed, fixed in 4% formaldehyde with mesh cassettes (Trajan), and further processed for immunohistochemistry.

Sawaki D., Zhang Y., Mohamadi A., Pini M., Mezdari Z., Lipskaia L., Naushad S., Lamendour L., Altintas D.M., Breau M., Liang H., Halfaoui M., Delmont T., Surenaud M., Rousseau D., Yoshimitsu T., Louache F., Adnot S., Henegar C., Gual P., Czibik G, & Derumeaux G. (2023). Osteopontin promotes age-related adipose tissue remodeling through senescence-associated macrophage dysfunction. JCI Insight, 8(8), e145811.

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Matrigel Implantation of Progenitor Cells

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Eight–ten-week-old WT male mice were anesthetized and shaved on the left flank and abdomen before sterilization of the surgical site. Recipients for these experiments were syngeneic with donors. A 5-µL Matrigel plug (Corning, 356231) containing 8,000 to 10,000 sorted Ctsk-lineage PSCs, PP1, or PP2 cells was implanted underneath the renal capsule. Animals were killed by CO₂ after 3 wk. Kidneys were fixed with 4% PFA for 5 h and subjected to infiltration, embedding, and sectioning. Von Kossa (Sigma-Aldrich) staining was performed to detect mineralized nodule formation. Quantification of Von Kossa staining was performed using Image J (NIH.gov, version 1.53t).

Chen R., Dong H., Raval D., Maridas D., Baroi S., Chen K., Hu D., Berry S.R., Baron R., Greenblatt M.B, & Gori F. (2023). Sfrp4 is required to maintain Ctsk-lineage periosteal stem cell niche function. Proceedings of the National Academy of Sciences of the United States of America, 120(46), e2312677120.

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