S camptothecin

Manufactured by Merck Group

Sourced in Germany, Italy

(S)-(+)-camptothecin is a natural compound isolated from the Chinese tree Camptotheca acuminata. It is a chemical compound that serves as a laboratory reagent and research tool. The core function of (S)-(+)-camptothecin is to inhibit the enzyme topoisomerase I, which is involved in DNA replication and transcription.

Automatically generated - may contain errors

Lab products found in correlation

Spelling variants of this product

Camptothecin (330 citations) (S)-(+)-camptothecin (CPT; (3 citations)

19 protocols using s camptothecin

Cellular Stress Induction and G-quadruplex Targeting

Check if the same lab product or an alternative is used in the 5 most similar protocols

Where indicated, cells underwent to following treatments: Cobalt Chloride (CoCl₂; Sigma Aldrich) 100 μM for 16 h; Heparin (PharmaTex, Milan, Italy) 200 ng/ml for 16 h; Heparinase II from Flavobacterium heparinum (Sigma Aldrich) 15 mU/ml for 2 h; (S)-(+)-camptothecin 0.2 μM for 2 h (CPT, Sigma Aldrich); the ATM-inhibitor KU-55933 (Sigma-Aldrich) was used at 5 μM for 24 h. G-quadruplex ligands Emicoron (33 (link)) and RHPS4 (34 (link)), were used at 1 μM for 24 and 72 h, respectively. SULF2 inhibitor 2,4-disulfonylphenyl-tert-butylnitrone (OKN-007, R&D systems) was dissolved in water and used at 50 mg/kg once a day for 2 weeks.

Zizza P., Dinami R., Porru M., Cingolani C., Salvati E., Rizzo A., D’Angelo C., Petti E., Amoreo C.A., Mottolese M., Sperduti I., Chambery A., Russo R., Ostano P., Chiorino G., Blandino G., Sacconi A., Cherfils-Vicini J., Leonetti C., Gilson E, & Biroccio A. (2019). TRF2 positively regulates SULF2 expression increasing VEGF-A release and activity in tumor microenvironment. Nucleic Acids Research, 47(7), 3365-3382.

+ Open protocol

+ Expand

Protein-Ligand Binding Interactions Study

Check if the same lab product or an alternative is used in the 5 most similar protocols

Alternariol (AOH) was obtained from Cfm Oskar Tropitzsch (Marktredwitz, Germany). Racemic warfarin (WAR), naproxen (NAP), human serum albumin (HSA), bovine serum albumin (BSA), porcine serum albumin (PSA), rat serum albumin (RSA), glimepiride (GLIM), furosemide (FUR), bilirubin (BIL), phenylbutazone (PBut), indomethacin (IME), racemic ibuprofen (IBU) and S-camptothecin (CPT) were purchased from Sigma-Aldrich (Budapest, Hungary). Ethinylestradiol (EE) was purchased from Serva (Budapest, Hungary). Spectroscopic grade ethanol (96%), as well as HPLC-grade acetonitrile and methanol were obtained from VWR (Budapest, Hungary). Stock solutions of AOH (5000 μM), bilirubin (500 μM), methyl orange (2000 μM), and glimepiride (2000 μM) were prepared in spectroscopic grade dimethyl sulfoxide (DMSO; Fluka, Bucharest, Romania). Stock solutions of indomethacin and ethinylestradiol (both 2000 μM), as well as ibuprofen, furosemide, phenylbutazone, and naproxen (each 2500 μM) were prepared in ethanol (96%, spectroscopic grade). The applied amounts of organic solvents did not affect significantly the fluorescence measurements (tested in each spectroscopic model). All stock solutions were stored at −20 °C, and protected from light.

Fliszár-Nyúl E., Lemli B., Kunsági-Máté S., Dellafiora L., Dall’Asta C., Cruciani G., Pethő G, & Poór M. (2019). Interaction of Mycotoxin Alternariol with Serum Albumin. International Journal of Molecular Sciences, 20(9), 2352.

+ Open protocol

+ Expand

Cytotoxicity Assay Reagents Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

(S)-(+)-camptothecin (#C9911), Triton X-100 (#X100), paraformaldehyde (#P6148), potassium phosphate monobasic (#P5655), sodium phosphate dibasic (#S5136), and dimethyl sulfoxide (DMSO, #D8418) were purchased from Sigma-Aldrich (Prague, Czech Republic); staurosporine (#3510A) was purchased from Santa Cruz Biotechnology (Heidelberg, Germany); Hoechst 33342 (#17530) was purchased from AAT Bioquest (Sunnyvale, CA, USA); and fetal bovine serum (#16000–036) was purchased from Thermo Fisher Scientific (Waltham, MA, USA). Hydrogen peroxide (30% solution, #23980–11000), potassium chloride (#60130G1000), sodium chloride (#71380G1000), isopropyl alcohol (#17510), nitric acid 65% (#18980) and sulfuric acid 96% (#20450) were obtained from Penta (Prague, Czech Republic).

Filipova M., Elhelu O.K., De Paoli S.H., Fremuntova Z., Mosko T., Cmarko D., Simak J, & Holada K. (2018). An effective “three-in-one” screening assay for testing drug and nanoparticle toxicity in human endothelial cells. PLoS ONE, 13(10), e0206557.

+ Open protocol

+ Expand

Synthesis of Dendrimer-Drug Conjugates

Check if the same lab product or an alternative is used in the 5 most similar protocols

EDA core PAMAM dendrimer G4.5 caboxylate sodium salt was purchased from Dendritech (Midland, MI). 2,2’-Bipyridine, 4-dimethylaminopyridine (DMAP), copper(II) sulfate (CuSO₄), (+)-sodium L-ascorbate, 4-(4,6-dimethoxy-(1,3,5)triazin-2-yl)-4-methyl-morpholinium chloride (DMTMM), deuterated solvents, dichloromethane (DCM), dimethyl sulfoxide (DMSO), and other organic solvents were purchased from Acros (Morris Plains, NJ). (S)-(+)-Camptothecin (CPT), propargylamine (PPA), copper bromide (CuBr), N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC), trifluoroacetic acid (TFA) and silica gel 60 (40-63 μm, 230-400 mesh) were purchased from Sigma-Aldrich (St. Louis, MO). 1-Azido-3,6,9,12,15-pentaoxaoctadecan-18-oic acid (APO) and methoxypoly(ethylene glycol) amine (mPEG-NH₂, 2000 g/mol) were purchased from Biomatrik (Jiaxing, Zhejiang, China) and JenKem Technology USA (Plano, TX), respectively. SnakeSkin dialysis tubing 3.5 kDa and 7 kDa MWCO, acetonitrile (ACN), HPLC grade water, phosphate-buffered saline (PBS), and magnesium sulfate (MgSO₄) were purchased from Thermo Fisher Scientific (Pittsburg, PA).

Zolotarskaya O.Y., Xu L., Valerie K, & Yang H. (2015). Click synthesis of a polyamidoamine dendrimer-based camptothecin prodrug. RSC advances, 5(72), 58600-58608.

+ Open protocol

+ Expand

Versatile Live-Cell Imaging Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were seeded and transfected 24 h prior to live imaging in the presence of different siRNAs (30-50 nM) using RNAiMAX (Invitrogen). EGFP-PCNA transfection was performed 24 h prior to imaging using FugeneHD (Promega). The following inhibitors were used: olaparib (AZD2281, Ku-0059436; Selleckchem), veliparib (ABT-888; Selleckchem), talazoparib (BMN 673; Selleckchem); XAV-939 (Selleckchem), ME328 [34 (link)], verapamil (Sigma), MG132 (Sigma), hydroxyurea (Sigma), cisplatin (CPDD, Sigma), (S)-(+)-camptothecin (Sigma), etoposide (Sigma). SiR-Hoechst (Life Technologies) was used at the final concentration of 500 nM.

Kukolj E., Kaufmann T., Dick A.E., Zeillinger R., Gerlich D.W, & Slade D. (2017). PARP inhibition causes premature loss of cohesion in cancer cells. Oncotarget, 8(61), 103931-103951.

+ Open protocol

+ Expand

Pharmacological Modulation of Hypoxia

Check if the same lab product or an alternative is used in the 5 most similar protocols

ATM inhibitor (KU-55933, Sigma-Aldrich) was treated both in zebrafish and cell cultures at 10 uM concentration. HIF activators (FG 4592, Stratech or JNJ-42041935, Johnson and Johnson) were treated at 15–50 uM and 100 uM respectively in zebrafish and in cell cultures. CPT ((S)-(+)-Camptothecin, Sigma Aldrich) was treated at 10–25 nM concentration in zebrafish and in cell cultures as indicated in the text.

Kim H.R., Santhakumar K., Markham E., Baldera D., Greenald D., Bryant H.E., El-Khamisy S.F, & van Eeden F.J. (2020). Investigation of the role of VHL-HIF signaling in DNA repair and apoptosis in zebrafish. Oncotarget, 11(13), 1109-1130.

+ Open protocol

+ Expand

Nanoparticle-Based Camptothecin Delivery

Check if the same lab product or an alternative is used in the 5 most similar protocols

(S)-(+)-Camptothecin (CPT ≥ 90%), human holo-Transferrin (Tf, ≥98%), tetraethyl orthosilicate (TEOS, 98%), (3-aminopropyl)triethoxysilane (APTES, ≥98%), cetyltrimethylammonium chloride solution (CTAC, 25 wt.%), ammonium hydroxide solution (acs reagent, 28–30%), and glutaraldehyde solution (GLUT, 25 wt.%), were purchased from Sigma-Aldrich. Ethanol (EtOH, HPLC grade), dimethyl sulfoxide (DMSO, for analysis), and hydrochloric acid (HCl, ACS reagent, fuming, 37%) were obtained from Merck. All other reagents and solvents were of analytical grade. Deionized water (Milli-Q, 18.2 MΩ·cm) was used in all experiments. All materials were used without any further purification.

Jackson N., Ortiz A.C., Jerez A., Morales J, & Arriagada F. (2023). Kinetics and Mechanism of Camptothecin Release from Transferrin-Gated Mesoporous Silica Nanoparticles through a pH-Responsive Surface Linker. Pharmaceutics, 15(6), 1590.

+ Open protocol

+ Expand

Inhibitors and Reagents for Cell Studies

Cited 2 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

MG132 was from Calbiochem. S-(+)-Camptothecin, cycloheximide, and naphthol AS-E were
from Sigma. LBL1 and LBL1-P were synthesized
as previously
described.^{29 (link),30 (link)}

Li B.X., Chen J., Chao B., Zheng Y, & Xiao X. (2018). A Lamin-Binding Ligand Inhibits Homologous Recombination Repair of DNA Double-Strand Breaks. ACS Central Science, 4(9), 1201-1210.

+ Open protocol

+ Expand

Clonogenic Assay for Cytotoxicity

Check if the same lab product or an alternative is used in the 5 most similar protocols

Clonogenic assays were performed as reported previously [23 (link)]. Briefly, hydrogen peroxide, methyl viologen dichloride hydrate (paraquat) and (S)-(+)-camptothecin (all from Sigma) were applied to HeLa cells for 1 day, followed by recovery for 7 days in clastogen-free medium and then incubation with 0.05% crystal violet in 2% ethanol for 20 min. A colony was defined as that containing more than 50 cells visualized under a phase contrast microscope.

Zhao C., Zeng H., Wu R.T, & Cheng W.H. (2016). Loss of Selenium-Binding Protein 1 Decreases Sensitivity to Clastogens and Intracellular Selenium Content in HeLa Cells. PLoS ONE, 11(7), e0158650.

+ Open protocol

+ Expand

Fission Yeast Stress Response Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols

Schizosaccharomyces pombe strains used in this study are listed in Supplemental Table S1. Basic manipulations and MM media composition are described elsewhere (21 ). Hydroxyurea (Sigma-Aldrich) was used at 40 mM in YES (Bacto™ Yeast extract (BD) 0.5%, glucose 3%, leucine 200 mg/l, uracil 100 mg/l, adenine 200 mg/l and histidine 200 mg/l) and yeast were incubated for 4 h to elicit Cds1 activation. Bleomycin hydrochloride (Wako) was used at 5 μg/ml in YES and incubated 1 h to induce Chk1 activation. (S)-(+)-Camptothecin (Sigma-Aldrich) was used at 500 nM in SD (Difco™ Yeast Nitrogen Base w/o Amino Acids (BD) 0.67%, glucose 1% and appropriate nutrients) plates to exclude self-circularized chromosome survivors in the screen for stn1-1 suppressor strains.

Takikawa M., Tarumoto Y, & Ishikawa F. (2016). Fission yeast Stn1 is crucial for semi-conservative replication at telomeres and subtelomeres. Nucleic Acids Research, 45(3), 1255-1269.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!