4T1 cells were seeded into 6-well plates at a density of 1 × 105 cells/mL (2 mL per well), followed by a 48 h incubation with 4-HIL dissolved in PBS. The cells were treated with RNase and Propidium iodide staining solution and incubated in the dark for at least 30 min at room temperature according to the manufacturer's instructions. Subsequently, the samples were analyzed by flow cytometry within 1 h. In order to quantify the apoptosis rate after treatment, the cells were stained with Annexin V-FITC and PI (MultiSciences #70-101-100) for 30 min at room temperature according to the manufacturer's instructions and the apoptosis rate was quantified by flow cytometry.
Annexin 5 fitc and pi
Manufactured by MultiSciences Biotech
Sourced in China
Annexin V-FITC and PI is a lab equipment product that serves as a fluorescent staining solution. Annexin V is conjugated with the fluorescent dye FITC, while propidium iodide (PI) is a separate component. This product is commonly used in flow cytometry analysis to detect and quantify apoptosis in cell samples.
Automatically generated - may contain errors
Lab products found in correlation
4 protocols using annexin 5 fitc and pi
1
Apoptosis Assay of 4T1 Cells
2
Cell Cycle and Apoptosis Analysis by Flow Cytometry
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Harvested cells were treated by being fixed overnight in ice-cold 75% ethanol at 4 °C, for analysis of the cell cycle. The fixed cells were washed twice with PBS and stained with propidium iodide (PI) (7seapharmtech, China). After staining, cell cycle analysis was performed by FACS scanning flow cytometry. For cell apoptosis analysis, cells at the logarithmic growth stage were harvested and washed twice with PBS. The collected cells underwent staining with Annexin V-FITC and PI (MULTISCIENCES, China) in darkness for 15 min. Following washing, flow cytometry detected apoptosis. The analysis of the data was conducted by FlowJo software (FlowJo).
3
PANC-1 Apoptosis Assay by Flow Cytometry
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The PANC-1 cells after 48 h of culture were collected by centrifugation, re-suspended in binding buffer. To this, Annexin V-FITC and PI (5 μL, Multi Sciences, China) was added and kept at room temperature for 15 min. Apoptosis was evaluated by flow cytometric analysis using a FACSCanto™II spectrophotometer (BD Biosciences, USA).
4
Apoptosis Quantification in Cultured Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
CCs (1 3 10 6 ) were collected after being treated with hyaluronidase without EDTA and were washed twice in PBS. To quantify cell death arising from apoptosis, an Annexin V-FITC/ PI apoptosis kit (Multisciences, China) was applied to examine the number of cells at different stages. Briefly, cells were resuspended in 0.5 mL binding buffer, and 5 mL of Annexin V-FITC and PI (Multisciences) were added to each tube. Cells were incubated for 10 minutes at 25°C in the dark before being analyzed by flow cytometry (FACS Calibur; BD Biosciences).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!