All standards (acetic acid, propionic acid, butyric acid, cholic acid (CA), deoxycholic acid (DCA), lithocholic acid (LCA), hyodeoxycholic acid (HDCA), dehydrocholic acid (DHCA), taurolithocholic acid (TLCA), and glycochenodeoxycholic acid (GCDCA)) were purchased from Aladdin Biochemical Technology Co., Ltd. (Shanghai, China). Formic acid, methanol, sulfuric acid, diethyl ether, ammonium acetate, and acetonitrile (HPLC grade) were obtained from McLean Biochemical Technology Co., Ltd. (Shanghai, China). Isomaltulose was provided by BENEO GmbH (Mannheim, Germany).
Methanol
Manufactured by Maclin Biochemical Technology
Sourced in China
Methanol is a colorless, flammable liquid chemical compound with the formula CH3OH. It is used as a solvent, fuel, and raw material in the production of other chemicals.
Automatically generated - may contain errors
Lab products found in correlation
Acetonitrile, by Maclin Biochemical Technology (24 mentions)
Acetic acid, by Maclin Biochemical Technology (20 mentions)
Formic acid, by Maclin Biochemical Technology (17 mentions)
Sulfuric acid, by Maclin Biochemical Technology (11 mentions)
Ethanol, by Maclin Biochemical Technology (10 mentions)
Sodium hydroxide (naoh), by Maclin Biochemical Technology (6 mentions)
Hydrochloric acid (hcl), by Maclin Biochemical Technology (5 mentions)
N n dimethylformamide (dmf), by Maclin Biochemical Technology (5 mentions)
Ethyl acetate, by Maclin Biochemical Technology (4 mentions)
Chloroform, by Maclin Biochemical Technology (3 mentions)
Heptadecanoic acid, by Maclin Biochemical Technology (3 mentions)
Pyridine, by Maclin Biochemical Technology (3 mentions)
O methylhydroxylamine hydrochloride, by J&K Scientific (3 mentions)
Dichloromethane, by Maclin Biochemical Technology (3 mentions)
Anhydrous ethanol, by Maclin Biochemical Technology (3 mentions)
Dimethyl sulfoxide (dmso), by Maclin Biochemical Technology (3 mentions)
Acetonitrile, by Thermo Fisher Scientific (3 mentions)
Cetyltrimethylammonium bromide, by Tianjin Damao (3 mentions)
Sulfuric acid, by Tianjin Damao (3 mentions)
Acetonitrile, by Sinopharm (3 mentions)
74 protocols using methanol
1
Quantification of Bile Acids in Biological Samples
2
Echinacoside and Acteoside Extraction
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The echinacoside (ECH, ≥98%) was supplied by the Shanghai Li Ding Biotechnology Co., Ltd. The acteoside (ACT, ≥98%) was purchased from the Shanghai Li Ding Biotechnology Co., Ltd. (Shanghai, China). 4-vinylpyridine (4-VP, 98%) and methacrylic acid (MAA, 98%) were purchased from the Aladdin Reagent Co., Ltd. (Shanghai, China). Ethyleneglycol dimethacrylate (EGDMA, 98%) was supplied by the Qingdao Ruinasi Polymer Material Co., Ltd (Qingdao, China), azodiisobutyronitrile (AIBN, 98%) was purchased from the Aladdin Reagent Co., Ltd. (Shanghai, China). N, N-dimethylformamide (DMF, 99.5%) was purchased from the Aladdin Reagent Co., Ltd. (Shanghai, China). Acrylamide (AM, 99%) was purchased from the Beijing Dingguo Biological Technology Co., Ltd. (Beijing, China). Acetonitrile (ACN, ≥99.9%) was supplied by Sinopharm Chemical, Reagent (Shanghai, China), Methanol (≥99.9%), and acetic acid (≥99.9%) were purchased from the Shanghai McLean Biochemical Technology Co., Ltd. (Shanghai, China). Ethanol (≥99.7%) was purchased from the Shanghai Shiyang Chemical Co., Ltd (Shanghai, China). Polyvinylidene fluoride membranes (PVDF membranes, 0.45 μm) were purchased from the RisingSun Membrane Technology Co., Ltd. (Beijing, China). Distilled water was supplied by the Smart-S15 system (Shanghai, China).
3
Extraction and Characterization of Brown Rice Bioactives
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The Changlixiang brown rice was provided by Northeast Agricultural University, China. The ultrasonic equipment (BILON-1000CT, 20KHz) was provided by Shanghai bilang instrument manufacturing Co., LTD, China. The cellulase (700 U/g) was obtained from Furuidemu Biotechnology Co., LTD, China. The sodium bicarbonate and phosphate buffer were obtained from Beijing Solarbio Technology Co., LTD, China. The phytic acid, γ-oryzanol, Gamma-aminobutyric acid (GABA), phenolic acid and flavonoid standards were purchased from Shanghai Yuanye Biotechnology Co., LTD, China. The methanol, ethyl alcohol, acetonitrile and acetic acid used in the study were chromatographic grades and purchased from Shanghai Maclin Biochemical Technology Co., LTD, China.
4
Extraction and Purification of HYPs from Shiraia bambusicola
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HYPs were produced by fermentation of Shiraia bambusicola (GDMCC 60438) described by Yan et al. [47 (link)]. After fermentation, the HYPs were extracted from the collected mycelia with dichloromethane (analytical reagent) and purified by recrystallization to a purity of 95% (with 95.7% HA and 2.9% HB, w/w). RhB (≥95%) and RhB standards (analytical standard, ≥97%) were purchased from Merck KgaA (Darmstadt, Germany). Methanol (HPLC grade, ≥99%) was provided by Macklin Biochemical Co., Ltd. (Shanghai, China). The H2O2 (30%, v/v, Fengchuan Chemical Reagent Co., Ltd., Tianjin, China), 1.3-butanediol (99%, Shanghai Aladdin Biochemical Technology Co., Ltd., China), ethyl acetate (Analytical Reagent, ≥99.5%), sodium dihydrogen phosphate (Analytical Reagent, ≥99%) and disodium hydrogen phosphate (Analytical Reagent, ≥99%, Damao Chemical Reagent Company, Tianjin, China) were all analytical grade.
5
Synthesis of Rare-Earth Chloride Complexes
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YCl3.·6H2O (99.99%, metal basis), YbCl3.·6H2O (99.99%, metal basis), TmCl3·6H2O (99.99%, metal basis), NH4, NaOH and oleic acid were purchased from Aladdin Reagent Co., Ltd. 1-Octadecene, methanol and cyclohexane were purchased from Shanghai Macklin Biochemical Co., Ltd. cyclohexane (99.5%) and tris(hydroxymethyl)aminomethane (>99.8%) were purchased from Sigma Aldrich. NaCl (≥99.5%) was purchased from Carl Roth. DDM (>99%, n-dodecyl-β-d -maltoside) was purchased from Glycon Biochemicals. MES (2-(N-morpholinyl)ethanesulfonic acid) were purchased from Sigma Aldrich. All chemicals were used without additional purification. Millipore Type 1 water (18.2 MΩ·cm) was used throughout the study.
6
Synthesis of High-Performance Flame-Retardant Composites
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5,5′-Oxybis (isobenzofuran-1,3-dione) (ODPA) was supplied by Illinois Ark Pharm Co., Ltd. (Libertyville, IL, USA). USA. p-Phenylenediamine (PPDA) and methanol were acquired from Shanghai Macklin Biochemical Co., Ltd. (Shanghai, China). N-methylpyrrolidinone (NMP) was purchased from Shanghai Aladdin industrial Co., Ltd. (Shanghai, China). NMP was distilled and then stored with 4Å molecular sieves. Before use, dianhydride and diamine were dried under primary vacuum at 100 °C for 12 h. PPDA was recrystallized twice. All solvents and reagents were analytical class.
The granuliform polyamide 6 (1013B) was supplied by Sinopec Shijiazhuang Refining & Chemical Company (Shijiazhang, Hebei, China). Aluminum diethylphosphinate (ADP, OP935) was purchased from Clariant Chemical Co., Ltd. (Shanghai, China). Antioxidant 1010 and antioxidant 168 were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Short glass fiber (GF, 568H) was produced by Jushi Group Co., Ltd. (Jiaxing, Zhejiang, China).
The granuliform polyamide 6 (1013B) was supplied by Sinopec Shijiazhuang Refining & Chemical Company (Shijiazhang, Hebei, China). Aluminum diethylphosphinate (ADP, OP935) was purchased from Clariant Chemical Co., Ltd. (Shanghai, China). Antioxidant 1010 and antioxidant 168 were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Short glass fiber (GF, 568H) was produced by Jushi Group Co., Ltd. (Jiaxing, Zhejiang, China).
7
Quantifying Cell Invasion Potential
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Cell invasion was measured using 24-well Transwell Chamber (8-μm pore membrane, BD Biosciences). 1×105 cells were harvested and planted into the Matrigel pre-coated upper chamber with 100 μL serum-free medium while the bottom chamber was filled with 600 μL completed medium. Inserts were incubated at 37°C, 5% CO2 for 24h. Non-invading cells were removed with a cotton swab soaked in medium. Cells that had moved through the pores (to the lower surface of the filters) were fixed with methanol (Macklin) for 1 h and stained with crystal violet (Solarbio) for 30 min, then counted invasive cells on a microscope at ECLIPSE Ti-U epi-fluorescence microscope (Nikon). Three inserts were counted for each treatment in each experiment and experiments were carried out at least three times.
8
Synthesis of Zinc Oxide Nanoparticles
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2-Methylimidazole, methanol, iodine (I2), polyvinylpyrrolidone (PVP, K30), polyacrylonitrile (PAN, average Mw 15 000), dimethyl sulfoxide (DMSO) and cyclohexane were purchased from Shanghai Macklin Biochemical Technology Co., Ltd. Zinc acetate dihydrate ((CH3COO)2Zn·2H2O) was purchased from Tianjin Fuchen Chemical Reagent Factory.
9
Optimized Protein Purification and Analysis
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DNA restriction enzymes, DNA Ligation high, Taq DNA polymerase, DNA marker, and protein marker were purchased from the Beyotime Institiute of Biotechnology (Shanghai, China). The Gel Extraction kit and Plasmid Miniprep kit were purchased from TIANGEN biotech (Beijing) Co., Ltd., (Beijing, China). Ni Sepharose™ 6 Fast Flow was purchased from GE Healthcare (Fairfield, MA, USA).
Ethyl acetate, n-butanol, and methanol were bought from Macklin (AR, Shanghai Macklin Biochemical Co. Ltd., Shanghai, China). NaCl, HCl, NaOH (AR, China National Pharmaceutical Group Corporation, Beijing, China), and distilled water were used. Chromatographic-grade methanol from Fisher (HPLC, Fisher Scientific, Waltham, MA, USA) was used for high performance liquid chromatography (HPLC) analysis. Paeoniflorin standards were purchased from YuanYe company (Shanghai, China).
Ethyl acetate, n-butanol, and methanol were bought from Macklin (AR, Shanghai Macklin Biochemical Co. Ltd., Shanghai, China). NaCl, HCl, NaOH (AR, China National Pharmaceutical Group Corporation, Beijing, China), and distilled water were used. Chromatographic-grade methanol from Fisher (HPLC, Fisher Scientific, Waltham, MA, USA) was used for high performance liquid chromatography (HPLC) analysis. Paeoniflorin standards were purchased from YuanYe company (Shanghai, China).
10
Lipidomic Analysis Using Synthetic Standards
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The PL standards were purchased from Avanti Polar Lipids, including phosphatidylcholine (PC 17:0/17:0), phosphatidylethanolamine (PE 17:0/17:0), phosphatidylglycerol (PG 17:0/17:0), phosphatidic acid (PA 17:0/17:0), phosphatidylinositol (PI 8:0/8:0), sphingomyelin (SM d18:1/12:0) and SPLASH LIPIDOMIX (isotopic internal standard mixture). Glyceryl trilinoleate (TAG) was purchased from Sigma Aldrich. Other chemicals, including methanol, chloroform, acetonitrile and ammonium formate, were purchased from Macklin Biochemical Technology Co., Ltd. (Shanghai, China) (high-performance liquid chromatography or analytical reagent grade).
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