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2 protocols using palatinose hydrate

1

Characterization of Carbohydrate Standards

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The following compounds were purchased from Sigma-Aldrich: D-glucose ≥ 99.5%, D (−) fructose ≥ 99%, D (+) Turanos ≥ 98%, erlose ≥ 94%, isomaltotriose, D (+) melibiose ≥ 99,0%, D (+), raffinose pentahydrated ≥ 98.0%, palatinose hydrate ≥ 99%, sucrose ≥ 99.5%, D (+) maltose monohydrate min 98%, melezitose ≥ 99.0%, trehalose dihydrate (Certified Reference Materials), D-panose ≥ 88 97%, maltotriose, isomaltose 98%,ascorbic acid, gallic acid, 2,2-diphenyl-1 picrylhydrazyl (DPPH), Folin–Ciocalteu’s reagent, hexamethyldisilazane ≥ 99%, trifluoroacetic acid, hydroxylamine hydrochloride, acetic anhydride, pyridine, potassium hexacyanoferrate (II) trihydrate, and zinc acetate dihydrate. Methanol, sulfuric acid, ethanol, and acetonitrile were purchased from Merck (Darmstadt, Germany). Aluminum chloride, sodium carbonate, sodium hydroxide, and sodium nitrite were purchased from Merck (Darmstadt, Germany). All chemicals used were of analytical grade.
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2

Honey Authentication via Carbohydrate Profiling

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Maltulose monohydrate (99.3%) was purchased from Chem-Impex Intl. (Wood Dale, IL). Sucrose (99.5%), α-lactose monohydrate (99%), maltose monohydrate (99%), palatinose hydrate (>98%), and D-(+)-turanose (>98%) were purchased from Sigma-Aldrich (St. Louis, MO). Phenylboronic acid (PBA, >97%) was obtained from Strem Chemicals (Newburyport, MA). Acetonitrile (HPLC grade) and ammonium hydroxide (35% solution in water) were purchased from Fisher Scientific (Pittsburgh, PA). High purity deionized water with 18.2 MΩ·cm resistivity was prepared using a Milli-Q filtration system (Merck Millipore, Burlington, MA). High-purity nitrogen was used as the sheath gas.
Honey samples were all US Grade A and consisted of Nature Nate’s (McKinney, TX) Natural 100% Raw Unfiltered Honey (honey #1), locally sourced raw honey from Union County, OH (honey #2), and Kirkland’s Signature (Costco Wholesale, Seattle, WA) Organic Raw Honey (honey #3).
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