Mm 102

GV oocytes from wild-type mice were cultured in M16 medium containing IBMX (both Sigma) at 37°C, 6% CO₂ and 5% O₂, and supplemented with: BIX-10294 (Stratech) at final concentrations of 0.1 µM and 1 µM (Huang et al., 2017 (link)); MM-102 (Cayman Chemicals) at final concentrations of 6 µM or 60 µM, within the range previously reported (Zhang et al., 2018 (link)); or a combination of 1 µM BIX-01294 and 60 µM MM-102. The inhibitors were dissolved in DMSO (Sigma) and an equivalent concentration was used for the control groups. After 48 h the surviving GV oocytes were either fixed in 4% paraformaldehyde (Thermo Scientific) for investigation by IF, or frozen at −80°C for RNA isolation and subsequent qRT-PCR.

Authenticated MCF7, T47D, MCF10A, HCC1500, and 293T cells were purchased from ATCC and used for experiments within the first 10 passages. Cultures were checked for Mycoplasma every 6 months (Lonza) and cells maintained at 37°C and 5% CO₂ in RPMI or DMEM + 10% FBS and 1% Pen-Strep. GDC-0941, BYL719, GDC-0032, and GDC-0068 were purchased from Selleck, and MI-136, MI-503, and MM-102 were purchased from Cayman. siRNAs (Silencer Select) were purchased from Invitrogen (Negative control #1, 4390843; siKMT2D, s528766).