Model 300c

Manufactured by Aurora Scientific

The Model 300C is a laboratory instrument designed to measure force and displacement. It features a high-resolution load cell and a precision linear actuator for accurate and reliable measurements.

Automatically generated - may contain errors

Lab products found in correlation

Model 400a, by Aurora Scientific (1 mentions) Matlab software, by MathWorks (1 mentions) Labview, by National Instruments (1 mentions)

Spelling variants of this product

Model 300C-LR (11 citations) Model 300C servomotor (2 citations) Model 129 300C-LR (2 citations) 300C L-R model (2 citations)

3 protocols using model 300c

Diaphragm Muscle Contractility Measurement

Check if the same lab product or an alternative is used in the 5 most similar protocols

The diaphragm strip measurement began with whole-tissue removal while the mouse was under anesthesia. In a dissection bath (136.5 mM NaCl, 5 mM KCl, 1.8 mM CaCl₂, 0.5 mM MgCl₂, 0.4 mM NaH₂PO₄, and 11.9 mM NaHCO₃), sutures were tied around both ends of a small strip cut along the myofibers of the diaphragm. The strip was placed in a bath (121 mM NaCl, 5 mM KCl, 1.8 mM CaCl₂, 0.5 mM MgCl₂, 0.4 mM NaH₂PO₄, 24 mM NaHCO₃, and 5.5 mM glucose), and the sutures were tied to the servomotor (model 300-C, Aurora Scientific) and force transducer. A similar procedure as for the TA muscle strength assay was used, with the stimulation voltage and then muscle length adjusted for maximum isomeric twitch force, and the TA length was measured (L0). Tetanus (the point at which maximum tension is generated) was measured at increasing stretch of L0: 0%, 5%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, and 45%. The signal output of the force transducer was displayed on a storage oscilloscope (Tektronix model 5111) coupled to a microcomputer used for data acquisition and storage.

Benasutti H., Maricelli J.W., Seto J., Hall J., Halbert C., Wicki J., Heusgen L., Purvis N., Regnier M., Lin D.C., Rodgers B.D, & Chamberlain J.S. (2023). Efficacy and muscle safety assessment of fukutin-related protein gene therapy. Molecular Therapy. Methods & Clinical Development, 30, 65-80.

+ Open protocol

+ Expand

Isolated Ventricular Trabeculae Contractility

Check if the same lab product or an alternative is used in the 5 most similar protocols

Thin, unbranched, and intact trabeculae were carefully dissected from the right ventricular wall and mounted between a force transducer (Cambridge Technology, model 400A) and a length-controlling motor (Aurora Scientific, model 300C). Each end of the trabecula was sutured to custom arms attached to the motor and force transducer made from 22-gauge needles. The trabecula was then submerged in a custom experimental chamber that was continuously perfused with modified Krebs buffer (1.8 mM CaCl₂) at 30°C. Twitches were elicited by field stimulation with custom platinum plate electrodes at 1 Hz with oscillating polarity. Sarcomere length (SL) was set to 2.0 μm using an inverted stereomicroscope with a ×40 dry objective lens and a ×10 eyepiece. If sarcomeres could not be seen for direct measurement (e.g., if the trabecula was too thick), then an SL of 2.0 μm was assumed to be at trabecula slack length (the length of the trabecula at the onset of passive T development). Trabeculae were allowed to pace at 1 Hz for approximately 20 minutes at SL 2.0 μm (and 30°C) and then stretched to SL 2.3 μm for data acquisition.
Continuous twitch T-time traces were recorded using custom LabView software at a sampling rate of 1 kHz and were analyzed using custom code with MATLAB software (version 2018a, The MathWorks).

Powers J.D., Kooiker K.B., Mason A.B., Teitgen A.E., Flint G.V., Tardiff J.C., Schwartz S.D., McCulloch A.D., Regnier M., Davis J, & Moussavi-Harami F. (2020). Modulating the tension-time integral of the cardiac twitch prevents dilated cardiomyopathy in murine hearts. JCI Insight, 5(20), e142446.

+ Open protocol

+ Expand

Muscle Fiber Mechanical Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols

Muscle fibers were attached to hooks with glue made from cellulose nitrate dissolved in acetone. One hook was attached to a dual-mode force/length servo-motor (Aurora Scientific Inc., model 300C) mounted on an X-Y-Z positioner. The other hook was attached to another X-Y-Z positioner that is adjusted to take up the slack in the fiber and then remains fixed during force measurements. Length was controlled and force measured using a National Instruments 16 bit digital/analog controller system controlled by custom software written in Labview (National Instruments Inc.). Sarcomere lengths were measured using laser diffraction with a helium-neon laser with a wavelength of 663nm.

Yuan C.C., Ma W., Schemmel P., Cheng Y.S., Liu J., Tsaprailis G., Feldman S., Southgate A.A, & Irving T.C. (2015). Elastic Proteins in the Flight Muscle of Manduca sexta. Archives of biochemistry and biophysics, 568, 16-27.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!