The cells were collected, fixed in ice-cold 70% (v/v%) ethanol, and incubated at −20 °C overnight. The samples were thawed, washed with 1% BSA (w/v%, dissolved in PBS), and incubated with primary antibodies (100 µL/2–5 × 105 cells) against γH2AX (1:500, phospho S139, 9F3, 1 mg/mL, Abcam) or Ki-67 (1:250, SP6, 300 µg/mL, Thermo Fisher Invitrogen) diluted in 1% BSA for 30 min at room temperature. The cells were incubated with secondary antibodies (1:1000, Alexa Fluor Plus 488—anti-mouse; Alexa Fluor 660—anti-rabbit, 2 mg/mL, Thermo Fisher Invitrogen) diluted in 1% BSA for 30 min at room temperature and resuspended in 1% BSA with a concentration of 5 × 105 cells/500 µL. For DPP4/CD26 staining, the cells were collected and resuspended in 1% BSA dissolved in PBS with a concentration of 3–5 × 105 cells/sample and stained with 100 µL staining solution with CD26-PE antibody (1:100, 2A6, 0.1 mg/mL, Invitrogen) diluted in 1% BSA for 30 min on ice. The cells were washed and resuspended in 1% BSA. 2 × 104 single cells were recorded using the Attune NxT Flow Cytometer. The results were analysed using FlowJo software (v10.8.0).
Alexa fluor 660 anti rabbit
Manufactured by Thermo Fisher Scientific
Alexa Fluor 660—anti-rabbit is a fluorescent dye-conjugated antibody. It is designed for use in immunofluorescence and other fluorescence-based applications.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using alexa fluor 660 anti rabbit
1
Immunophenotyping of Cellular Stress and Proliferation
2
Quantitative Flow Cytometry Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The cells were collected, fixed in ice-cold 70% (v/v%) ethanol, and incubated at -20 • C overnight. The samples were thawed, washed with 1% BSA (w/v%, dissolved in PBS), and incubated with primary antibodies (100 µL/2-5 × 10 5 cells) against γH2AX (1:500, phospho S139, 9F3, 1 mg/mL, Abcam) or Ki-67 (1:250, SP6, 300 µg/mL, Thermo Fisher Invitrogen) diluted in 1% BSA for 30 min at room temperature. The cells were incubated with secondary antibodies (1:1000, Alexa Fluor Plus 488-anti-mouse; Alexa Fluor 660-anti-rabbit, 2 mg/mL, Thermo Fisher Invitrogen) diluted in 1% BSA for 30 min at room temperature and resuspended in 1% BSA with a concentration of 5 × 10 5 cells/500 µL. For DPP4/CD26 staining, the cells were collected and resuspended in 1% BSA dissolved in PBS with a concentration of 3-5 × 10 5 cells/sample and stained with 100 µL staining solution with CD26-PE antibody (1:100, 2A6, 0.1 mg/mL, Invitrogen) diluted in 1% BSA for 30 min on ice. The cells were washed and resuspended in 1% BSA. 2 × 10 4 single cells were recorded using the Attune NxT Flow Cytometer. The results were analysed using FlowJo software (v10.8.0).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!