Immunohistochemistry staining was performed according to the standard protocol as previous described [23 ]. Polyclonal rabbit primary antibody for Flot2 (Abcam, San Francisco, CA), HRP-conjugated secondary antibody and DAB staining kit (CWBIO, Beijing, China) were used in the experiment. The intensity of staining was scored as 0 (negative), 1 (weak), 2 (medium) or 3 (strong), while the extent of staining was scored as 0 (0% of cell stained), 1 (1–25%), 2 (26–50%), 3 (51–75%) or 4 (76–100%). Then the intensity and the extent scores were multiplied as the final staining scores (0–12) for Flot2 expression. Tumors of final staining score 0–2 were considered as negative, 3–5 as low expression, and 6–12 as high expression.
Dab staining kit
Manufactured by CWBIO
Sourced in China
The DAB staining kit is a laboratory tool used for the detection and visualization of target proteins or antigens in biological samples through a chromogenic reaction. The kit provides the necessary reagents and solutions to perform the diaminobenzidine (DAB) staining process, which results in the formation of a brown-colored precipitate at the site of the target molecule. The core function of the DAB staining kit is to enable the localization and identification of specific proteins or antigens within a sample, as part of various immunohistochemical or immunocytochemical analysis techniques.
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Lab products found in correlation
2 protocols using dab staining kit
1
Immunohistochemical Evaluation of Flot2
2
Immunofluorescence Staining of GLP-1R
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Immunofluorescence staining was performed according to the standard protocol described previously (Liao et al., 2017 (link)). Polyclonal rabbit primary antibody to GLP-1R (Bioss, bs-1559R; 1:100), HRP-conjugated secondary antibody and DAB staining kit (CWBIO, Beijing, China) were used in the experiment. Images were captured with a laser confocal microscope (FV10-ASW, Olympus, Tokyo, Japan).
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