Bcas2

The 40-μm floating sections (series of every 12th section from the hippocampus) underwent IFA as described (4) and were incubated with the antibodies β-catenin (BD; 1:500), BCAS2 (Proteintech, Rosemont, IL, USA; 1:10000), Cre (Abcam, Cambridge, UK; 1:500), Sox2 (Abcam; 1:500), BrdU (Abcam; 1:500), NeuN (Millipore, NJ, USA; 1:1000), DCX (Millipore, Darmstadt, Germany; 1:500), GFAP (Sigma; 1:500), GAD67 (Abcam; 1:250), and cyanine Cy3 goat anti-mouse IgG, Alexa Fluor 488 donkey anti-rabbit IgG, Alexa Fluor 647 goat anti-rat igG, Alexa Fluor 488 donkey anti-rat (all Jackson ImmunoResearch PA, USA; 1:1000) (Additional file 2: Table S1). Images were captured by using a Leica TSC SP5 confocal microscope.

Protein lysates were extracted from cells or brain tissues and underwent western blot analysis with the antibodies β-catenin (BD, San Jose, CA, USA; 1:5000), p-GSK3β (BD; 1:5000), β-actin (Sigma-Aldrich, St. Louis, MO, USA; 1:10000), BCAS2 (Proteintech, Rosemont, IL, USA; 1:10000), Flag (Sigma-Aldrich; 1:10000), anti-mouse IgG (Sigma-Aldrich; 1:10000), anti-rabbit IgG (Sigma-Aldrich; 1:10000).