The AIH test is a widely used test for the detection of antimanic activity (Pereira et al., 2014 (link)). This test was developed based on the verification that amphetamine can induce mania-like symptoms in healthy individuals and mood stabilizers, as lithium and valproate, can reverse such amphetamine effects (Willson et al., 2005 (link); Gould et al., 2007 (link)). Briefly, rats were placed in a square (1 x 1 m) arena with a video camera mounted above the arena. Baseline locomotor activity was recorded for 45 min, after which rats received an i.p. injection of amphetamine (1 mg/kg) and were placed back into the arena to record locomotor activity for a further 45 min. One group of rats received a saline injection (and tap water oral gavage) to demonstrate amphetamine-induced hyperlocomotion. The distance moved (cm) by each rat was measured using EthoVision XT video tracking software (version 12; Noldus Information Technology, Waacheningen, The Netherlands), and the data were collapsed into 5-min bins.
Ethovision xt video
Manufactured by Noldus
EthoVision XT is a video tracking software designed for behavioral research. It allows for automated tracking and analysis of animal movement and behavior within a specified arena or environment.
Automatically generated - may contain errors
Lab products found in correlation
6 protocols using ethovision xt video
1
Amphetamine-Induced Hyperlocomotion Assay
2
Open Field Test for Locomotor Activity
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Locomotor activity was measured using the open field test. Mice were individually placed in a white square box (40 × 40 × 40 cm, Center: ~ 5 lx; Periphery: ~ 4 lx) by facing the wall and kept for 30 min. The locomotor activity and center duration of the mice were videotaped and analyzed by the Noldus EthoVision XT video tracking system.
3
Open Field Exploration and Novel Object Recognition
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Mice were tested in a square open field arena (50 × 50 cm) (Panlab, Spain) located in a room with dim lighting, as described previously.41 Briefly, after habituation to the OF arena (10 min/day over 2 days; no objects), the mice were placed in the open field with two identical objects for 10 minutes. To assess long‐term memory, 24 hours later the mice were placed again in the OF arena for 5 minutes and one of the familiar objects was replaced by a novel one. To record and analyze behavior, the EthoVision XT video tracking system was used (Noldus Information Technology).
4
Behavioral Phenotyping of Rodent Models
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Testing took place in three parallel batches with distinct test orders. Animals never performed more than one test a day and between each behavioural test animals were allowed to rest for 3–5 days to reduce stress and support recovery e.g. following weight loss from food deprivation. All behavioural testing took place between 10:00 and 16:00 h. Experimenters were blind to the genotype of the rats during all experimental sessions. If not otherwise stated all behavioural data were collected using EthoVisionXT video tracking equipment and software (Noldus Information Technology) at a rate of 5 frames per second. Learning was assessed using the Morris water maze, radial arm maze and discrimination reversal. Open field analysis, novel object recognition and sucrose consumption tests were included to assess factors that may interfere with learning performance such as locomotion, anxiety, novelty response and taste perception.
5
Novel Object Recognition in Mice
6
Morris Water Maze for Spatial Memory
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The procedure of MWM consisted of 5-day learning and memory training trials, and a probe trial on day 6, performed one month after STZ-icv administration, in a 180-cm-diameter round pool, 60 cm deep, with water temperature set at 25±1°C. On days 1-5, rats were trained to escape the water by nding a hidden platform (15-cm diameter) submerged about 2 cm below the water surface and placed in the Northwest/NW quadrant. Remaining on the platform in order to memorize its location was allowed for 5 s. Four consecutive trials were performed per day, each from a different starting position (Southwest/SW, South/S, East/E and Northeast/NE), separated by a 30-min rest period. The time needed to nd the platform, number of non-target entries (entries into the quadrants other than the NW quadrant in which the platform was located) and rat swimming velocity were recorded during the training trials, which tested the capacity of learning memory. A probe trial which tested memory retention was performed (from quadrant SouthEast/SE) with the platform removed from the pool, where the time spent in search of the platform within the NW quadrant, rat swimming velocity and non-target entries were recorded. The cut-off time was 1 min. The data was recorded, tracked and analyzed using the EthoVision XT video tracking software (Noldus Information Technology).
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