Bs 200 auto analyzer

Manufactured by Mindray

Sourced in China

The BS-200® auto analyzer is a clinical chemistry analyzer designed for routine testing in medical laboratories. It performs automated biochemical analysis of various samples, including blood, urine, and other bodily fluids. The BS-200® auto analyzer is capable of measuring a wide range of analytes, providing accurate and reliable results to support clinical decision-making.

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Lab products found in correlation

K 4500, by Sysmex (1 mentions)

Close spelling variants of this product

BS-200 (86 citations)

7 protocols using bs 200 auto analyzer

Kidney Histopathology and Biochemical Analysis

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Standard kits and a Mindray BS-200^® auto analyzer were employed to assess serum gamma-glutamyl transpeptidase (γ-GT), cratinine (Cr), and blood urea nitrogen (BUN) [17 (link)]. For histopathological assessments, samples of kidney tissue were fixed in a buffered formalin solution (0.4% of sodium phosphate monobasic, NaH₂PO₄, 0.64% of sodium phosphate dibasic, Na₂HPO₄, and 10% of formaldehyde in distilled water; pH = 7.4). Finally, paraffin-embedded sections of tissue were prepared and stained with hematoxylin and eosin (H&E) before light microscope viewing [18 (link)].

Niknahad H., Heidari R., Mohammadzadeh R., Ommati M.M., Khodaei F., Azarpira N., Abdoli N., Zarei M., Asadi B., Rasti M., Shirazi Yeganeh B., Taheri V., Saeedi A, & Najibi A. (2017). Sulfasalazine induces mitochondrial dysfunction and renal injury. Renal Failure, 39(1), 745-753.

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Evaluating Fasting Lipid and Hepatitis Profiles

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Following 12 h of fasting, a venous blood sample was drawn from each participant to determine the fasting blood sugar (FBS) and lipid profiles. According to the protocol using the BS200 Auto analyzer (Mindray, China), all tests such as FBS, triglyceride, high-density lipoprotein (HDL), and total cholesterol, were assessed enzymatically. Serum low-density lipoprotein (LDL) cholesterol was calculated using the Friedewald equation (23 (link)).
Acon kits (Acon Laboratory, San Diego, CA92121, USA) were used to evaluate hepatitis B virus (HBV) biomarkers such as HbsAg, HBsAb, and HBcAb using the third generation Enzyme-Linked Immuno-Sorbent Assay (ELISA) method.
The Iranian National Reference Laboratory re-evaluated 10% of the blood samples, and a variation coefficient of 1.7–3.8% was considered acceptable for all laboratory values.

Doustmohammadian A., Pishgar E., Clark C.C., Sobhrakhshankhah E., Nikkhah M., Faraji A.H., Motamed N., Mansourian M.R., Amirkalali B., Maadi M., Kasaii M.S., Ebrahimi H, & Zamani F. (2022). Empirically-Derived Dietary Patterns in Relation to Non-Alcoholic Fatty Liver Diseases Among Adult Participants in Amol, Northern Iran: A Structural Equation Modeling Approach. Frontiers in Nutrition, 9, 821544.

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Quantifying Brain Ammonia in Cirrhosis

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A Mindray BS-200 autoanalyzer (Mindray chemistry analyzers for low-volume laboratories, Guangzhou, China) and standard commercial kits (Pars Azmun, Tehran, Iran) were used to measure serum albumin, bilirubin, alanine aminotransferase (ALT), aspartate aminotransferase (AST), and lactate dehydrogenase (LDH) [51 (link)]. Plasma ammonia level was measured with standard kits based on the absorbance photometry method of phenate-hypochlorate reaction [52 (link)]. Brain ammonia level of cirrhotic animals was determined according to a previously reported method [44 (link)] (Table 1). Briefly, forebrain (cerebral cortex) samples (100 mg) were dissected, homogenized, and deproteinized in 3 ml of an ice-cooled (4°C) lysis solution (trichloroacetic acid, 6% w/v in double distilled water). After centrifugation (12,000 g, 10 minutes, 4°C), the supernatant was collected and neutralized (KHCO₃; 2 mol/l, pH = 7). Afterward, the ammonia content of the supernatant was measured using standard kits [52 (link)].

Heidari R., Jamshidzadeh A., Ghanbarinejad V., Ommati M.M, & Niknahad H. (2018). Taurine supplementation abates cirrhosis-associated locomotor dysfunction. Clinical and Experimental Hepatology, 4(2), 72-82.

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Lithium Impacts on Leydig Cell LDH

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A commercial kit (Pars Azmun^®, Tehran, Iran) and Mindray^® BS-200 autoanalyzer (Guangzhou, China) were used to assess cells lactate dehydrogenase (LDH) levels in cultured LCs (95 (link), 96 (link)). Isolated LCs were treated with different concentrations of Li⁺ (0, 10, 50, and 100 ppm) after the incubation period. Afterward, 200 μL of the culture medium was taken, and the LDH content was measured.

Ommati M.M., Arabnezhad M.R., Farshad O., Jamshidzadeh A., Niknahad H., Retana-Marquez S., Jia Z., Nateghahmadi M.H., Mousavi K., Arazi A., Azmoon M.R., Azarpira N, & Heidari R. (2021). The Role of Mitochondrial Impairment and Oxidative Stress in the Pathogenesis of Lithium-Induced Reproductive Toxicity in Male Mice. Frontiers in Veterinary Science, 8, 603262.

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Comprehensive Blood Biomarkers Analysis

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After 12 hours of fasting, 10 cc whole blood samples were collected in each time from the patients during the follow-up. EDTA containing vacuum tubes (Terumo Europe, Leuven, Belgium) and serum separator tubes (SST, tiger top tube) were used for sample collection. A BS200 auto-analyzer (Mindray, China) was used to measure alanine aminotransferase (ALT), aspartate aminotransferase (AST), creatinine, BUN, alkaline phosphatase (ALP), total and direct bilirubin, albumin, and ferritin. An automated cell counter (Sysmex K-4500, Sysmex, Japan) was used to assess hemoglobin (Hb), platelet (PLT), and white blood cell count (WBC) as well as complete blood cell count (CBC). Also, other hematological tests, including prothrombin time (PT) and partial thromboplastin time (PTT), were performed. In addition, other demographic data were collected using a questionnaire and medical data repository, including age, sex, and splenectomy and treatment history.

Safarnezhad Tameshkel F., Karbalaie Niya M.H., Khoonsari M., Ajdarkosh H., Faraji A.H., Nikkhah M., Motamed N., Azarkeivan A., Gholami A., Sohrabi M.R., Keyvani H, & Zamani F. (2021). Effect of Baseline Resistance-Associated Substitutions on Thalassemia Patients with Chronic HCV Infection: A Two-Year Follow-Up. Middle East Journal of Digestive Diseases, 13(1), 27-34.

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Assessing Liver Damage Biomarkers in Rats

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After performing motor co-ordination tests to determine the biochemistry of blood, animals were anesthetized with thiopental (80 mg/kg, i.p). Then, blood was collected from the abdominal aorta, moved to standard tubes (Heparin-coated tubes), and centrifuged (3000 g, for 15 minutes, 4 °C) to prepare plasma. Standard kits (Biorexfars, Shiraz, Iran) and a Mindray BS-200^® auto analyzer was used to analyze plasma biomarkers of liver damage such as aspartate aminotransferase (AST), lactate dehydrogenase (LDH), alanine aminotransferase (ALT), and bilirubin. For histopathological assessments, samples of liver were fixed in buffered formalin solution (0.4% sodium phosphate monobasic, NaH₂PO₄, 0.64% sodium phosphate dibasic, Na₂HPO₄, and 10% formaldehyde in distilled water).45 (link) Paraffin-embedded sections of tissue (5 µm) were prepared and stained with hematoxylin and eosin (H&E) before light microscope viewing.

Mohammadi H., Heidari R., Niknezhad S.V., Jamshidzadeh A, & Farjadian F. (2020). In vitro and in vivo Evaluation of Succinic Acid-Substituted Mesoporous Silica for Ammonia Adsorption: Potential Application in the Management of Hepatic Encephalopathy. International Journal of Nanomedicine, 15, 10085-10098.

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Fasting Blood Lipid Profiling

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Totally, 10 ml of whole blood was obtained from each participant using a serum separator tube (SST; tiger top tube). Following 12‐h fasting, FBS, and lipid profiles were evaluated. All the tests, including FBS, triglycerides (TG), HDL, LDL, and cholesterol were enzymatically assessed using the BS200 Auto analyzer (Mindray).

Motamed N., Ajdarkosh H., Karbalaie Niya M.H., Panahi M., Farahani B., Rezaie N., Nikkhah M., Faraji A.H., Hemmasi G., Perumal D., Ashrafi G.H., Safarnezhad Tameshkel F., Gholizadeh E., Khoonsari M, & Zamani F. (2022). Scoring systems of metabolic syndrome and prediction of cardiovascular events: A population based cohort study. Clinical Cardiology, 45(6), 641-649.

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