Quorum q150r

To enhance the understanding of extraction process variances, the microstructures of untreated purple-fleshed sweet potato, as well as those subjected to conventional and ultrasound-assisted extraction (UAE), were examined using a scanning electron microscope (JSM-6010 LA, Jeol, Tokyo, Japan). Initially, samples were affixed to metal supports (stubs) using carbon tape and subsequently coated with a thin layer of gold utilizing a sputtering apparatus (Quorum Q150R, Quorum Technologies Ltd., East Sussex, UK). The samples were then scanned using SEM under partial vacuum conditions at 10 kV.

To visualize PRP on the coated and uncoated sutures, three suture samples were used for the scanning electron microscopy study (None-coated, SC-coated and PRP-coated). Suture samples were cut into 2mm sections, allowed to air-dry at room temperature and coated in gold Quorum Q150R (Quorum Technologies Ltd, UK). Samples were then analysed using Quanta FEG 450 (Thermo Fisher Scientific, USA).

The Energy Dispersive X-ray Spectroscopy (EDS) analysis was performed using a Bruker X-flash 630 detector (Baden-Württemberg, Germany) with a resolution of 123 eV inside the Scanning Electron Microscopy (SEM) chamber in an approximate area of 104 μm × 104 μm. The fibers were characterized by SEM using TESCAN MIRA 3 equipment (Tescan, Brno, South Moravian, Czech Republic), for which the sample was coated with approximately 20 nm of gold (99.99% purity) with a Quorum Q150R sputtering evaporator (Quorum Technologies, Lewes, UK) and using a secondary electron detector with an accelerating voltage of 10 kV. In addition, Differential Scanning Calorimetry (DSC) experiments were carried out on a STAR Default Db Mettler Toledo (Mettler Toledo, Milan, Italy), to characterize temperature transition in each material subject to the same condition. Temperature ranged from 20 °C to 290 °C, with a heating rate of 10 °C/min.

A high-resolution scanning electron microscope Vega 3 (Tescan, Brno, Czech Republic) was used for nanofibrous mats characterization. First, the samples were sputtered by a conductive coating layer using a sputter Quorum Q150R (Quorum Technologies Ltd., Laughton, UK). A mean nanofiber diameter derived from 300 measurements taken from three different images was determined by applying the Adobe Creative Suite software (San Jose, CA, USA).

The surface morphology was evaluated using scanning electron microscopy (SEM; Hitachi S-3400 N; Hitachi High-Technologies Scientific Instruments, Wokingham UK), at an accelerating voltage of 5 kV. Prior to observation, each sample was sputtered with gold in a Quorum Q150R ion sputter (Quorum Technologies, Lewes, UK) for 90 s. Porosity was analysed and averaged based on 30 measurements from the SEM images using Image Tool (UTHSCSA; Image Tool Version 2; University of Texas, TX, USA).

A high-resolution scanning electron microscope Vega 3 (Tescan, Czech Republic) was used for characterization of nanofibrous mats. Prior to imaging, the samples were sputtered by a conductive coating layer using a sputter Quorum Q150R (Quorum Technologies Ltd., Laughton, UK).