Anti-caspase-8 monoclonal antibody C15 recognizes the p18 subunit of caspase-8. Anti-FLIP monoclonal antibody NF6 recognizes the N-terminal part of c-FLIP. Anti-FADD monoclonal antibody 1C4 recognizes the C-terminal part of FADD46 (link). Antibodies against Caspase-3, caspase-9, p65, Phospho-NFκB p65 (Ser536), cIAP1, cIAP2, XIAP, BclXL, and Smac were obtained from Cell Signaling. Antibodies specific for Bcl-2 were purchased from Santa Cruz Biotechnology, RIPK1 from BD Bioscience, Trx and Tom20 from Abcam, β-Actin from Genetech and Tubulin from Sigma.
β actin
Manufactured by Gene Tech
Sourced in Hong Kong
β-actin is a highly conserved cytoskeletal protein that is essential for maintaining cell structure and function. It is a key component of the eukaryotic cytoskeleton and is involved in a variety of cellular processes, including cell motility, division, and signal transduction.
Automatically generated - may contain errors
Lab products found in correlation
Gapdh, by Gene Tech (2 mentions)
Odyssey clx imaging system, by Gene Tech (2 mentions)
Protein rapid extraction kit, by Keygen Biotech (2 mentions)
β actin, by Proteintech (2 mentions)
Bca protein assay, by Dingguo (2 mentions)
Ciap1, by Cell Signaling Technology (1 mentions)
Ciap2, by Cell Signaling Technology (1 mentions)
Phospho nf κb p65 ser536, by Cell Signaling Technology (1 mentions)
Bcl xl, by Cell Signaling Technology (1 mentions)
Ripk1, by BD (1 mentions)
3 protocols using β actin
1
Antibody-based detection of apoptosis pathway
2
Western Blot Protein Expression Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The whole cellular protein was extracted by a Protein Rapid Extraction kit (KeyGEN, China), and quantified by BCA protein assay (Dingguo, China). After denaturation, protein lysate was separated with SDS-PAGE, and transferred to a nitrocellulose membrane (Pall, Germany). The membrane was blocked with Tris-buffered saline (TBS) solution containing 5% skim milk powder for 1 h at room temperature. After wash three times with TBST (TBS with 0.05% Tween-20), membrane was incubated with the primary antibody diluted by TBST solution (1: 1000), including Bip (#3177, CST, USA), CHOP (#2895, CST, USA), CD36 (ab64014, Abcam, UK), GAPDH (#5174, CST, USA) and β-actin (66,009, Proteintech, USA) overnight. After three-time wash with TBST, membrane was incubated with the corresponding fluorescent secondary antibody (LI-COR, Gene Company Limited, Hong Kong) for 1 h at room temperature, and ultimately detected using Odyssey® CLx imaging system (Gene Company Limited, Hong Kong). At least three independent experiments were performed. The relative expression level of protein was analyzed by Image Studio™ quantification software (Gene Company Limited, Hong Kong) with β-actin or GAPDH as internal control, and normalized to the control group.
3
Western Blot Analysis of Protein Expression
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The whole cellular protein was extracted by a Protein Rapid Extraction kit (KeyGEN, China), and quanti ed by BCA protein assay (Dingguo, China). After denaturation, protein lysate was separated with SDS-PAGE, and transferred to a nitrocellulose membrane (Pall, Germany). The membrane was blocked with Trisbuffered saline (TBS) solution containing 5% skim milk powder for 1 hour at room temperature. After wash three times with TBST (TBS with 0.05% Tween-20), membrane was incubated with the primary antibody diluted by TBST solution (1 : 1000), including Bip (#3177, CST, USA), CHOP (#2895, CST, USA), CD36 (ab64014, Abcam, UK), GADPH (#5174, CST, USA) and β-actin (66009, Proteintech, USA) overnight.
After three-time wash with TBST, membrane was incubated with the corresponding uorescent secondary antibody (LI-COR, Gene Company Limited, Hong Kong) for 1 hour at room temperature, and ultimately detected using Odyssey® CLx imaging system (Gene Company Limited, Hong Kong). At least three independent experiments were performed. The relative expression level of protein was analyzed by Image Studio™ quanti cation software (Gene Company Limited, Hong Kong) with β-actin or GAPDH as internal control, and normalized to the control group.
After three-time wash with TBST, membrane was incubated with the corresponding uorescent secondary antibody (LI-COR, Gene Company Limited, Hong Kong) for 1 hour at room temperature, and ultimately detected using Odyssey® CLx imaging system (Gene Company Limited, Hong Kong). At least three independent experiments were performed. The relative expression level of protein was analyzed by Image Studio™ quanti cation software (Gene Company Limited, Hong Kong) with β-actin or GAPDH as internal control, and normalized to the control group.
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