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Murine recombinant ifnγ

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Murine recombinant IFNγ is a laboratory reagent produced through recombinant technology. It is a cytokine that plays a critical role in the immune response.

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Lab products found in correlation

Griess reagent system, by Promega (1 mentions) Lps eb ultrapure, by InvivoGen (1 mentions)

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Recombinant murine interferon-gamma (IFNγ) (9 citations)

2 protocols using murine recombinant ifnγ

1

Nitric Oxide Production Assay

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RAW264.7 cells were seeded in triplicate in 96-well plates at a density of 5 × 104 cells/well and incubated under standard conditions for 24 h. The cells were treated with 20 ng/mL of murine recombinant IFNγ (GIBCO, Gaithersburg, MD, USA), with or without (control) compounds at various concentrations for 24 h. The level of NO production was evaluated by determining the nitrite concentration in culture medium with a Griess Reagent System (Promega, Madison, WI, USA). The IC50NO value was calculated as the derivative concentration required to decrease NO production to 50% of the non-treated control.
Markov A.V., Babich V.O., Popadyuk I.I., Salomatina O.V., Logashenko E.B., Salakhutdinov N.F, & Zenkova M.A. (2019). Novel Derivatives of Deoxycholic Acid Bearing Linear Aliphatic Diamine and Aminoalcohol Moieties and their Cyclic Analogs at the C3 Position: Synthesis and Evaluation of Their In Vitro Antitumor Potential. Molecules, 24(14), 2644.
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2

Immune Response Modulation Protocol

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BMMO and BMDC were treated with BG at MOI 40 and 5, respectively or as indicated in the figure legends. Treatment with TLR4-specific lipopolysaccharide (LPS) from E. coli 0111:B4 strain (LPS-EB Ultrapure) (Invivogen; CA, USA), murine recombinant IFNγ (Gibco) were done at a concentration indicated in the figure legends.
Lim J., Koh V.H., Cho S.S., Periaswamy B., Choi D.P., Vacca M., De Sessions P.F., Kudela P., Lubitz W., Pastorin G, & Alonso S. (2019). Harnessing the Immunomodulatory Properties of Bacterial Ghosts to Boost the Anti-mycobacterial Protective Immunity. Frontiers in Immunology, 10, 2737.
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