Fmoc protected amino acid loaded resins

Manufactured by Merck Group

Sourced in Germany

Fmoc-protected amino acid-loaded resins are a type of lab equipment used in solid-phase peptide synthesis (SPPS). These resins consist of a solid polymeric support, typically polystyrene or polyethylene glycol, onto which Fmoc-protected amino acids are covalently bound. The Fmoc-protecting group allows for the controlled addition of amino acids during the peptide synthesis process. These resins provide a stable platform for the step-by-step assembly of peptide chains.

Automatically generated - may contain errors

Lab products found in correlation

Syro 1, by Biotage (2 mentions) Tof tof 5800, by AB Sciex (2 mentions) Fmoc protected amino acids, by Merck Group (2 mentions)

Close spelling variants of this product

Amino acids

2 protocols using fmoc protected amino acid loaded resins

Solid-Phase Peptide Synthesis and Characterization

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Peptides were synthesized by a standard solid-phase method using Syro I (Biotage, Uppsala, Sweden). Fmoc-protected amino acid-loaded resins and Fmoc-protected amino acids were purchased from Merck (Darmstadt, Germany). Additionally, 1-[Bis(dimethylamino)methylene]-1H-1,2,3-triazolo[4,5-b]pyridinium 3-oxide hexafluorophosphate (HATU, Merck) was used in the coupling reaction. After cleavage and deprotection of peptides using reagent K (trifluoroacetic acid/phenol/thioanisole/1,2-ethanedithiol, 82.5/5/5/2.5), cold diethyl ether was added to precipitate the peptides. Sequences were confirmed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) (TOF/TOF5800, AB SCIEX, Framingham, MA, USA).

Sun C., Nagaoka K., Kobayashi Y., Nakagawa H., Kakimi K, & Nakajima J. (2021). Neoantigen Dendritic Cell Vaccination Combined with Anti-CD38 and CpG Elicits Anti-Tumor Immunity against the Immune Checkpoint Therapy-Resistant Murine Lung Cancer Cell Line LLC1. Cancers, 13(21), 5508.

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Solid-Phase Peptide Synthesis Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Peptides were synthesized by standard solid-phase synthesis using a Syro I (Biotage, Uppsala, Sweden). Fmoc-protected amino acid-loaded resins and Fmoc-protected amino acids were purchased from Merck (Darmstadt, Germany). 1-[Bis(dimethylamino)methylene]-1H-1,2,3-triazolo[4,5-b]pyridinium 3-oxide hexafluorophosphate (HATU, Merck) was used in the coupling reaction. After cleavage and deprotection of peptides using reagent K (trifluoroacetic acid/phenol/thioanisole/1,2-ethanedithiol, 82.5/5/5/2.5), cold diethyl ether was added to precipitate the peptides. Sequences were confirmed by matrix-assisted laser desorption/ionization Time-of-flight mass spectrometry (MALDI-TOF MS) (TOF/TOF5800, AB SCIEX, Framingham, Massachusetts, USA).

Nagaoka K., Shirai M., Taniguchi K., Hosoi A., Sun C., Kobayashi Y., Maejima K., Fujita M., Nakagawa H., Nomura S, & Kakimi K. (2020). Deep immunophenotyping at the single-cell level identifies a combination of anti-IL-17 and checkpoint blockade as an effective treatment in a preclinical model of data-guided personalized immunotherapy. Journal for Immunotherapy of Cancer, 8(2), e001358.

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