Xevo g2 xs q tof instrument

Manufactured by Waters Corporation

Sourced in United States

The Xevo G2-XS Q-TOF instrument is a high-resolution, accurate-mass quadrupole time-of-flight mass spectrometer designed for a range of analytical applications. It provides accurate mass data and high-quality information for the identification and quantification of unknown compounds.

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Lab products found in correlation

Acquity beh300 c4 1.7 μm column, by Waters Corporation (2 mentions) Mass fragment software, by Waters Corporation (1 mentions) Kta fplc, by GE Healthcare (1 mentions) Sypro orange, by Thermo Fisher Scientific (1 mentions) Acquity uplc h class system, by Waters Corporation (1 mentions) Steponeplus rt pcr instrument, by Thermo Fisher Scientific (1 mentions) Superdex 200 increase 10 300 gl column, by GE Healthcare (1 mentions) Biacore 3000 system, by GE Healthcare (1 mentions) Uplc h class system, by Waters Corporation (1 mentions) Superdex 200 10 300 gl, by GE Healthcare (1 mentions)

Spelling variants of this product

Xevo G2-XS (37 citations) Xevo G2 ToF (10 citations) Xevo G2-XS TOF instrument (2 citations) XevoTM (2 citations) Xevo G2-XS Q-TOF MS instrument (2 citations)

6 protocols using xevo g2 xs q tof instrument

DESI-MS/MS Analysis of Phytohormones

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One μl of aqueous methanol (50%) containing 1 ng/μl of the ABA or OPDA standards were placed on a glass slide, and allowed to dry. The MS/MS spectrum of each compound was measured using a Xevo G2-XS Q-TOF instrument equipped with a 2D DESI ion source (all Waters, Milford MA, USA). The DESI spray solvent (95% methanol:5% water, v/v) was delivered at a flow rate of 3 μl/min using a Harvard syringe pump. A voltage of 3 kV was applied to the DESI sprayer, and nitrogen was delivered at 0.4 MPa from an external gas cylinder. The optimised CE voltages for the generation of major fragment ions were 10 eV for ABA and 20 eV for OPDA. External calibration was performed by 10 mmol/L sodium formate solution in 90% 2-propanol (v/v) prior to measurement. The chemical formulae and structures of the major fragment ions were calculated using the MassFragment software (Waters).

Enomoto H., Sensu T., Sato K., Sato F., Paxton T., Yumoto E., Miyamoto K., Asahina M., Yokota T, & Yamane H. (2017). Visualisation of abscisic acid and 12-oxo-phytodienoic acid in immature Phaseolus vulgaris L. seeds using desorption electrospray ionisation-imaging mass spectrometry. Scientific Reports, 7, 42977.

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Metabolic Profiling of Activated T Cells

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WT OT-I⁺ or Ch25h^−/− splenocytes were stimulated with OVA peptide (0.5μg/ml) for 48 hr followed by treatment with vehicle or TCM for 8 hr in vitro. These cells then were span down at 250g for 5 min followed by wash with 1ml PBS twice. After this, cell pellet was suspended in 1 mL of ice-cold (−48 °C) 80% (v/v) methanol: water followed by centrifuge the solution at −9°C, 11,500 g for 10 min. Finally, cells pellets were frozen in liquid nitrogen for 30s and stored until analysis. Samples were extracted using a biphasic extraction (Tambellini et al., 2013 (link)) and then subjected to lipidomics analysis by spotting on a 24 well PTFE slide and analysis by desorption electrospray ionization (Takats et al., 2004 (link)), on a Waters Xevo G2-XS qtof instrument. Pixels from technical replicates were averaged and 8564 features were detected. A targeted analysis was performed for 25HC m/z 385.3465, representing the [M+H-H₂0]⁺ ion of hydroxysterols (DeBarber et al., 2008 (link)).

Vijh S., Pilip I.M, & Pamer E.G. (1999). Noncompetitive Expansion of Cytotoxic T Lymphocytes Specific for Different Antigens during Bacterial Infection. Infection and Immunity, 67(3), 1303-1309.

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Characterizing Purified Protein Structure

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Purified proteins were analyzed by size-exclusion chromatography on a Superdex 200 increase 10/300 GL column on an ÄKTA FPLC (GE Healthcare, Amersham Biosciences). SDS-PAGE was performed with 10% gels (Invitrogen) under reducing and non-reducing conditions. For ESI-MS analysis samples were diluted to about 0.1 mg/mL and LC-MS was performed on a Waters Xevo G2XS Qtof instrument (ESI-ToF-MS) coupled to a Waters Acquity UPLC H-Class System using a 2.1 × 50 mm Acquity BEH300 C4 1.7 μm column (Waters). Differential scanning fluorimetry was performed on an Applied Biosystems StepOnePlus RT-PCR instrument. Protein samples were diluted at 2 μM in PBS in 40 μL and placed in PCR tubes, assay was performed in triplicates. 5x SYPRO ORANGE (Invitrogen, stock 5000x) was added to samples prior to analysis. For thermal stability measurements, the temperature range spanned from 25 to 95°C with a scan rate of 1°C/min. Data analysis was performed in Protein Thermal Shift™ Software version 1.3. The temperature derivative of the melting curve was computed.

De Luca R., Gouyou B., Ongaro T., Villa A., Ziffels B., Sannino A., Buttinoni G., Galeazzi S., Mazzacuva M, & Neri D. (2019). A Novel Fully-Human Potency-Matched Dual Cytokine-Antibody Fusion Protein Targets Carbonic Anhydrase IX in Renal Cell Carcinomas. Frontiers in Oncology, 9, 1228.

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DESI-MS Imaging of Biological Samples

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All experiments were performed with a Xevo G2-XS QTof instrument (Waters, Milford, MA, USA) operated in sensitivity mode and equipped with a two-dimensional DESI stage from Prosolia (Indianapolis, IN, USA) and a custom-built inlet capillary heated to 490 °C. DESI parameters were optimized for the best signal intensity on tissue and were as follows: spray voltage, 4.5 kV; solvent, 95:5 methanol–water; flow rate, 1.5 or 0.5 μl/min for pixel sizes of 100 μm or 50 and 20 μm respectively; nebulizing gas, nitrogen; gas pressure, 7 and 4.5 bar for 1.5 and 0.5 μl/min flow rate respectively; sprayer incidence angle, 75°; collection angle, 10°; sprayer-to-inlet distance, 10 mm; sprayer-to-sample distance, 1.5 mm. MS parameters were as follows: scan time (unless specified otherwise), 1 s; source temperature, 120 °C; sampling cone voltage, -40 V; source offset, -80 V.

Tillner J., Wu V., Jones E.A., Pringle S.D., Karancsi T., Dannhorn A., Veselkov K., McKenzie J.S, & Takats Z. (2017). Faster, More Reproducible DESI-MS for Biological Tissue Imaging. Journal of the American Society for Mass Spectrometry, 28(10), 2090-2098.

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Characterization of Antibody-Drug Conjugates

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All ADC products were analyzed by SDS-PAGE (Invitrogen), size exclusion
chromatography (Superdex200 10/300GL, GE Healthcare) and protein mass
spectrometry. Liquid chromatography-mass spectrometry (LC-MS) was performed on a
Waters Xevo G2-XS Qtof instrument (ESI-ToF-MS) coupled to a Waters Acquity UPLC
H-Class System using a 2.1 x 50 mm Acquity BEH300 C4 1.7 μm column
(Waters). 0.1% formic acid in water (solvent A) and 0.1% formic acid in
acetonitrile (solvent B) were used as mobile phase at a flow rate of 0.4 mL/min.
Gradient was programmed as follows: after 1.5 min isocratic with 95% solvent A,
stepwise change from 95% solvent A to 95% solvent B in 4.5 min (10% increase
every 0.5 min), back to 95% solvent A in 0.5 min, linearly to 95% solvent B and
back to 95% solvent A in 2.25 min (last step repeated twice). The binding
properties of SIP(F16)-MMAE, IgG(F16)-MMAE and IgG(F6)*-MMAE were analyzed by
surface plasmon resonance (BIAcore 3000 System, GE Healthcare) on an
TnC-A1-coated CM5 sensor chip (BIAcore) as previously described 15 (link).

Gébleux R., Stringhini M., Casanova R., Soltermann A, & Neri D. (2016). Non-internalizing antibody-drug conjugates display potent anti-cancer activity upon proteolytic release of monomethyl auristatin E in the sub-endothelial extracellular matrix. International journal of cancer, 140(7), 1670-1679.

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NMR Characterization of Organic Compounds

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¹H NMR spectra were recorded at 25 °C at 400 MHz. Chemical shifts are expressed in parts per million (ppm, δ scale) downfield from tetramethylsilane and are referenced to residual protium in the NMR (CDCl₃, δ 7.26; CD₃OD, δ 3.31). Data are represented as follows: chemical shift, multiplicity (s = singlet, d = doublet, t = triplet, q = quartet, m = multiplet and/or multiple resonances, br = broad, app = apparent), coupling constant in Hertz, and integration. Proton-decoupled carbon nuclear magnetic resonance spectra (¹³C NMR) were recorded at 100 MHz at 25 °C. Chemical shifts are expressed in parts per million (ppm, δ scale) downfield from tetramethylsilane and are referenced to the carbon resonances of the solvent (CDCl₃, δ 77.0; CD₃OD, δ 49.0). Fluorine nuclear magnetic resonance spectra (¹⁹F NMR) were recorded at 376 MHz at 25 °C. Chemical shifts are expressed in parts per million (ppm, δ scale) and are referenced indirectly to the ¹H frequency of CHCl₃. High-resolution mass spectrometry (HRMS) data were obtained using a Waters Xevo-G2 XS qTOF instrument.

Miller Jenkins L.M., Paine E.L., Deshmukh L., Nikolayevskiy H., Lyons G.C., Scerba M.T., Rosenker K.G., Luecke H.F., Louis J.M., Chertova E., Gorelick R.J., Ott D.E., Clore G.M, & Appella D.H. (2019). Inhibition of HIV Maturation via Selective Unfolding and Cross-Linking of Gag Polyprotein by a Mercaptobenzamide Acetylator. Journal of the American Chemical Society, 141(20), 8327-8338.

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