The monoclonal antibodies used in this study included CD3 PerCP, CD5 APC-R700, CD8-FITC, CD11c PE-Cy5, CD14 APC-H7, CD19 PE-Cy5, CD25 PE-Cy7, CD24 FITC, CD27 PE-Cy7, CD28 PE-Cy5, CD38 APC-H7, CD45 V500, CD45RO APC, CD56 APC, CD57 FITC, CD80 PE, CD86 APC, CD123 APC, CD127 BV421, CCR7 PE, TIGIT BV421, HLA DR V450, TIM3 PE, CXCR5 APC-R700, PD1 BV421, and PDL1 PE-Cy7 (BD Biosciences).
Cd38 apc h7
Manufactured by BD
Sourced in Spain, France
CD38-APC-H7 is a fluorescently-labeled antibody that binds to the CD38 cell surface antigen. It is designed for use in flow cytometry applications.
Automatically generated - may contain errors
Lab products found in correlation
Cd8 fitc, by BD (2 mentions)
Cd25 pe cy7, by BD (2 mentions)
Cd3 percp, by BD (2 mentions)
Cd24 fitc, by BD (2 mentions)
Cd127 bv421, by BD (2 mentions)
Pd l1 pe cy7, by BD (2 mentions)
Cd45ro apc, by BD (2 mentions)
Cd14 apc h7, by BD (2 mentions)
Cd80 pe, by BD (1 mentions)
Ccr7 pe, by BD (1 mentions)
Cd86 apc, by BD (1 mentions)
Cd27 pe cy7, by BD (1 mentions)
Cd11c pe cy5, by BD (1 mentions)
Tigit bv421, by BD (1 mentions)
Hla dr v450, by BD (1 mentions)
Tim 3 pe, by BD (1 mentions)
Pd 1 bv421, by BD (1 mentions)
Cd123 apc, by BD (1 mentions)
Cd19 pe cy5, by BD (1 mentions)
Cd57 fitc, by BD (1 mentions)
4 protocols using cd38 apc h7
1
Comprehensive Immune Cell Profiling
2
Multiparametric Immune Profiling
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CD8-FITC, CD20-FITC, CD24-FITC, interferon-gamma (IFNγ)-FITC, interleukin (IL)-1β-FITC, CD27-PE, IL-10-PE, CD197 (CCR7)-PE, GM-CSF-PE, CD3-PerCP, tumor necrosis factor-alpha (TNFα)-PerCP-Cy5.5, CD19-PE-Cy7, CD25-PE-Cy7, programmed death-ligand 1 (PD-L1)-PE-Cy7, CD45RO-APC, CD56-APC, IL-12-APC, IL-6-APC, CD4-APC-H7, CD8-APC-H7, CD14-APC-H7, CD38-APC-H7, CD3-BV421, CD127-BV421, IL-6-BV421, CD45-V500 (BD Biosciences, San Jose, CA), and IL-17-APC (R&D Systems, Minneapolis, MN).
3
Immunophenotyping of MM Cell Lines
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MM cell lines were immunophenotyped on a FACSCanto II cytometer (Beckton Dickinson Biosciences) using the following monoclonal antibodies: CD138-OC515 (Cytognos S.L., Salamanca, Spain), CD38-APC-H7 (BD Biosciences), and sIgk-PB (Vestec, Czech Republic). Data analysis was performed using the Infinicyt software (Cytognos S.L.). A minimum of 105 events were stored. Median fluorescence intensity of each marker was analyzed.
4
Identification of B cell subsets
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For the identification of B cell subsets, the following monoclonal antibodies were added to 250 μl of PB collected in K3-EDTA: CD20-PB (Pacific Blue; clone 2H7; BioLegend, San Diego, CA, USA); CD27-PC5 (phycoerythrin-cyanine 5; clone 1A4LDG5; Beckman Coulter; USA); CD19-PC7 (phycoerythrin-cyanine 7; clone J3-119; Beckman Coulter, France); CD45-KO (Krome Orange; clone J.33; Beckman Coulter, France); CD38-APC-H7 (allophycocyanin-hilite7; clone HB7; BD Biosciences, San Jose, CA, USA). The samples were then incubated for 15 min at room temperature and kept in the dark. To determine the immunoglobulin class, an intracytoplasmic staining was performed with the monoclonal antibodies IgG-FITC (fluorescein isothiocyanate, clone G18-145, BD Pharmingen, San Diego, CA, USA), IgA-PE (phycoerythrin, clone IS11-8E10, Macs Miltenyi Biotec, Bergisch Gladbach, Germany) and IgM-APC (allophycocyanin, clone G20-127, BD Pharmingen) using IntraPrep kit (Beckman Coulter, Brea, CA, USA) according to the supplier instructions. The cell pellet was resuspended in 0.5 ml of phosphate buffer saline (Gibco, Paisley, Scotland). The flow cytometry gating strategy to identify circulating B cell subsets is shown in Fig. 1.
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