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19 protocols using eos 400d

1

Photographic Setup for Natural Head Posture

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Data collection was done ‘in the field’ (non-clinical setting) at available classrooms designated by each school. The photographic set up consisted of a Canon EOS 400D (Canon, Shimomaruko, Ohta-ku, Tokyo, Japan) camera with Canon EF-S 60mm f/2.8 Macro USM Lens and Canon MR-14EX TTL Macro Ring lite Flash. Subjects were positioned against a scale backdrop of 1cm increment with plumbline which indicated the true vertical (TV). A vertical standing mirror was positioned out of the frame perpendicular to the left side of the background set up for improved reproducibility of the natural head posture [18 (link)].
Subjects were asked to remove glasses or other accessories, which may obstruct the profile. They were then instructed to a standing position, asked to relax with both arms hanging down at their sides and look straight into their eyes in the mirror with lips in a relaxed position. The right side profile was taken in natural head posture (NHP).
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2

Cone Calorimeter Burning Behavior Evaluation

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Burning behavior was evaluated by cone calorimeter examinations conducted on Fire Testing Technology apparatus (East Grinstead, UK,) following the ISO 5660-1 and ISO 5660-2 procedures. The horizontally oriented samples were irradiated at a heat flux of 35 kW/m2, and spark ignition was employed to ignite the pyrolysis products. An optical system with a silicon photodiode and a helium-neon laser provided a continuous survey of smoke. The burning process during tests was photographed using a digital camera, EOS 400 D, from Canon Inc. (Tokyo, Japan).
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3

Macroscopic Fungal Characterization Protocol

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Macroscopic characters were studied on different media and growth conditions. Cultures were plated onto Czapek yeast extract agar (CYA), CYA supplemented with 5 % NaCl (CYAS), yeast extract sucrose agar (YES), creatine sucrose agar (CREA), dichloran 18 % glycerol agar (DG18), oatmeal agar (OA) and malt extract agar (MEA; Oxoid malt). The isolates were inoculated at three points on 90 mm Petri dishes and incubated for 7 d at 25 °C in darkness. All media were prepared as described by Visagie et al. (2014b) (link). Additional CYA plates were incubated at 37 °C for 7 d in darkness. The isolates growing at 37 °C, were also incubated at 40 °C for 7 d in darkness. After incubation, the colony diameters on the various media were measured. The density of sporulation, obverse and reverse colony colours and the production of soluble pigments were noted. Colony colour codes refer to Kornerup & Wanscher (1967) . Colonies were photographed with a Canon EOS 400D. Species were characterised microscopically by preparing slides from MEA. Lactid acid was used as mounting fluid. Specimens were examined using a Zeiss AxioSkop2 plus microscope, and the NIS-Elements D software package from Nikon was used for capturing photographs and taking measurements.
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4

Aerial Imagery for Drought Assessment

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The image acquisition system from the ultra-light aircraft consisted of two commercial digital cameras (either Canon EOS 400D or 500D, with 10.1 and 15.1 Megapixel CMOS sensors, respectively, Table 1B) equipped with 35-mm lenses, and one FLIR B20HSV (FLIR Systems Inc., Wilsonville, USA) thermal infrared camera (320*240 matrix) (for details, see: Lebourgeois et al., 2008 , 2012 ; Virlet et al., 2014 (link)). One camera acquired visible images in red, green and blue bands (RGB). The second was modified according to Lebourgeois et al. (2008 , 2012 ) to obtain images in near-infrared (NIR). Three flights per year were performed during the summers of 2010 and 2011 (Table 1A, 1B). In 2010, flights were realized for low, intermediate and severe water constraints, respectively 8, 27 and 41d after the beginning of drought (Dates 1, 2 and 3). In 2011, the first flight (Date 4) occurred 17 d before the beginning of the drought period, before WW and WS differentiation, while the second and third flights (Dates 5 and 6) were performed respectively 14 and 34d after the beginning of the drought treatment. During the period of water deprivation (i.e. at Dates 1, 2, 3, 5 and 6) WS trees were not irrigated.
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5

Infrared Luminescence Imaging of Pigments

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Two flashes Quantum T5D mounted with B+W 486 UV/IR blocking filter were used for irradiating with visible light the surfaces of painted slabs, in order to explore the peculiar characteristics of some pigments to be luminescent in the infrared region when excited with visible light. The infrared emission was collected with a modified (built–in filter for IR removed) Canon EOS 400D (10.1 Mpixel, CMOS sensor) with Canon lens EFS 28 mm fitted with B+W 093 IR830 infrared filter to cut all stray radiation from visible spectrum and thus collecting only infrared luminescence emission. A white plate Spectralon® (WS-1S-L Labsphere certified standard) and a self-made mock up with Egyptian Blue were used as reference. In this context, such a technique represented a useful tool for the identification and localization of Egyptian Blue pigment [16 ].
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6

Flammability Evaluation of Composite Materials

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The flammability of the prepared composites was assessed based on the results of cone calorimetry measurements. All samples were tested using Fire Testing Technology Ltd., East Grinstead, UK, apparatus according to ISO 5660 standard. Samples of dimensions of 100 × 100 × 6 mm were placed in aluminum foil and irradiated horizontally at a heat flux of 35 kW m−2. The pyrolysis products that were released during tests were spark ignited. The images of the residues after the flammability tests were collected using a Canon Inc. EOS 400 D Tokyo, Japan, digital camera. The FIGRA (fire growth rate) was calculated based on Equation (1): FIGRA=pHRRTpHRR,
where pHRR is the maximum heat release rate and TpHRR is the time of the pHRR occurrences.
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7

Tadpole Morphometrics Using Digital Images

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Digital images were taken by placing a tadpole into a glass cuvette and photographing it from the side with a Canon EOS 400D camera (lens: Canon EF 28-90mm). tpsDIG2 (F. James Rohlf) software was used to take the following measurements from these images: total length, tail length, body height and maximum tail height.
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8

Fur Coloration Quantification via Digital Photography

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Digital photography was used to estimate animal fur colouration25 (link), 30 (link), 65 (link). Field and museum samples were photographed alongside a ColorChecker (X-Rite, Michigan, USA) with Canon EOS400D digital camera, 18–55 mm (set to 55 mm) 1:3.5–56 lens. Photographs were black and white-balance corrected (with GIMP 2.8 program) using the white and black references in the ColorChecker for light condition standardization. High-resolution images (TIFF format) were analysed with Hyper-Utility 2 program (www.fujifilm.com) to quantify fur colouration from a square-shaped area on the back of animal’s head (between the ears, pixel size: 40–150). To test for repeatability of colouration estimates (representativeness of dorsal colour), a total of 40 samples were randomly selected with “Sampling Design Tool” (in ArcGIS 10.166 ) on which selected areas for colour estimation were moved to different locations, and calculations were repeated. Red, green and blue reflectance and total reflectance were measured as Red-Green-Blue (RGB, 8 bit, 0–255) standard values.
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9

Quantifying Surface Wettability Changes

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The GM wettability before and after plasma treatment was measured via static water contact angle (WCA) measurement. GM substrates were placed on a custom stage and aligned with a glass magnifying lens (figure S2). A digital camera (EOS 400D, Canon, Melville, NY, USA) was then used to photograph the contour of the droplet above the patterned area. WCA measurements before and after oxygen plasma treatment (300 W, 150 sccm, 5 min) were recorded using ImageJ (NIH, Rockville, MD, USA). Three substrates of each depth were fabricated to record the relative change.
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10

Intraoral Photography of Dental Seals

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An intra-oral photograph using digital camera EOS 400D (Canon, Tokyo, Japan) with ring-flash and macrolens of 100 mm, f/2.8 features of the selected teeth, was taken by a trained photographer with assistance from one of the authors. Photographs of mandibular molars were taken, with the children seated on a chair, while the maxillary molars were photographed with the children lying on a table. Each photograph was judged for acceptability and quality and if not acceptable the photograph was retaken. The photographs were cropped to show only the sealed tooth and then randomly ordered to ensure that the identity of the material was not known to the evaluator.
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