Anti myc m20002

Anti-Moesin (ab151542) (1:1000), anti-Ezrin (ab4069) (1:200), anti-Radixin (ab52495) (1:1000), anti-phospho-ERM (ab76247) (1:1000), and anti-CD8α (ab209775) were purchased from Abcam. anti-Zap-70 (2705) (1:1000), anti-Syk (2712) (1:1000), anti-phospho-Erk1/2 (8544, 9101) (1:1000), anti-Erk1/2 (9102) (1:1000), anti-phospho-Akt (9271) (1:800), anti-Akt (9272) (1:1000), anti-phospho-P38 (9211) (1:1000), anti-P38 (9212) (1:1000), anti-rabbit-IgG (7074), and anti-mouse-IgG (7076) were purchased from CST. Anti-phospho-tyrosine (05–321) (1:1000) was purchased from Merck. Anti-GAPDH (BE0023) (1:3000) was purchased from EASYBIO. Anti-Myc (M20002) (1:1000), anti-Flag (M20008) (1:1000), and Protein A/G-Agarose (A10001) were purchased from Abmart. Anti-Flag M2 affinity gel (A2220) and uric acid (U2625) were purchased from Sigma-Aldrich. Polystyrene microspheres (19822) were purchased from Polysciences. Piceatannol (527948), Syk Inhibitor (574711), Syk Inhibitor IV, BAY 61-3606 HCL (574714), R406 (5.05819.0001), Wortmannin (681676), and LY294002 (440204) were purchased from Calbiochem. AZD0530 (S1006) and PP2 (S7008) were purchased from Selleck. Bodipy FL-PI(4,5)P2 (C-45F6) were purchased from Echelon Biosciences. TopFluor TMR-PC (810180C) and TopFluor TMR-PE (810241C) were purchased from Avanti Lipids. Geneticin (10131027) was purchased from ThermoFisher.