Instate software version 3

The results (symmetric with skewness values from 0 to 0.941) of the present study were expressed as mean ± S.E of seven rats. The parametric analysis, one-way analysis of variance (ANOVA) using Duncan’s test, was used to determine the difference between the mean values in all the studied groups. The analysis was done by SPSS software version 16 and the significance was considered at P-value < 0.05. The statistical significance between the studied variables was illustrated using different letters (a, b, c,…). The GraphPad Instate software version 3 was used to calculate the IC50 values for the in vitro antioxidant analyses.

The data are expressed as mean ± SE and the p-value < 0.05 is considered significant. The difference between the mean values of the studied groups was evaluated by one-way analysis of variance (ANOVA) by Tukey’s test. Before applying this parametric test, all data were checked for their normal distribution (skewness 0–0.868). The analysis was performed for seven rats using SPSS software version 16. The IC₅₀, EC₁₀₀, ED₅₀, and ED₁₀₀ values were calculated by GraphPad Instate software version 3. In addition, the CompuSyn software (ComboSyn, Inc, Paramus, NJ) accomplished the CI values, Fa-CI plots and isobologram plots for the in vitro antioxidant experiments.

The data are expressed as mean ± SE and the significance was set at p-value < 0.05. A one-way ANOVA (one-way analysis of variance) using Duncan's analysis, measured the difference between the mean values of the analyzed groups. All data were tested for their regular distribution (skewness 0–0.953) prior to applying this parametric test. The study was conducted using SPSS program version 16 and the IC50 values for the in vitro antioxidant assessments were calculated using the GraphPad Instate software version 3.