Cgp57380

CGP57380 and eFT508 were purchased from MedChemExpress (Monmouth Junction) and dissolved in 10% β-cyclodextrin (Sigma-Aldrich) for in vivo administration. Anti–PD-1 (clone RMP1-14), anti–CSF-1R (clone AFS98), and anti-CD8 (clone 2.43) mouse antibodies and their isotype-matched IgG control antibodies were purchased from Bio X Cell. Antibodies were stored undiluted at 4°C and upon treating animals were diluted in InVioPure pH 7.0 Dilution Buffer from Bio X Cell.

Mouse monoclonal anti-VSV-G (used at 1:1000) and HSV-ICP8 (1:1000) were obtained from Santa Cruz lnc. (Santa Cruz, CA, USA). The antibodies to actin (1:1000), goat anti-mouse secondary antibodies conjugated to horseradish peroxidase (HRP), and goat anti-rabbit secondary antibodies conjugated to HRP (1:10,000) were obtained from Beyotime Biotechnology (Haimen, China). The rabbit polyclonal antibody to phosphorylated (p)-eIF4E (Ser 209, 1:1000) was purchased from Cell Signaling Technology (Danvers, MA, USA). Rabbit polyclonal antibody to eIF4E (1:1000) was purchased from Proteintech (Wuhan, China). Mouse polyclonal antibody to PEDV-N (1:1000) was purchased from Alpha Diagnostic International (San Antonio, TX, USA). Rabbit polyclonal antibody to AIV-NP (1:2000) was purchased from Abcam (Cambridge, MA, USA). The antibodies to NDV-NP (1:2000) and PRV-gC (1:1000) were preserved in our laboratory. Antibodies were diluted in phosphate-buffered saline (PBS) containing 5% skim milk (pH 7.3). Ribavirin, acyclovir, quercetin, and the mitogen-activated protein kinase (MAPK)-interacting kinase 1 and 2 (MNK1/2) kinase inhibitors CGP57380 were obtained from MedChemExpress (Monmouth Junction, NJ, USA).