Hla dr bv605

Manufactured by BD

Sourced in Germany

HLA-DR BV605 is a fluorescently-labeled antibody that binds to the HLA-DR antigen, which is expressed on the surface of antigen-presenting cells. This product is intended for use in flow cytometry applications to identify and analyze HLA-DR-positive cells.

Automatically generated - may contain errors

Lab products found in correlation

6 protocols using hla dr bv605

Comprehensive Cytokine and T-cell Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols

Antibodies for intracellular cytokine staining included: anti-CD3-AF700, CD4-PcPCy5.5, CD8-PECF594, IFNg-FITC, CD14-V500, CD19-V500, TNFα-APC, CD38-PECy7, HLADR-BV605 (BD Biosciences); PD-1-PE, IL-2-BV421 (BioLegend); Live-Dead-AquaViD (LifeTechnologies).
The T regulatory cell/activation panel included: anti-CD3-AF700, CD25-PE, HLA-DR-FITC (BD); CD4-PETxR, CD8-APC AF750, Live-Dead-AquaViD (LifeTechnologies); CD39-PECy7, FOXP3-APC (eBioscience); CD127- PE Cy5.5 (Beckman Coulter); CD38- BV421 (BioLegend).

Barreto-Duarte B., Sterling T.R., Fiske C.T., Almeida A., Nochowicz C.H., Smith R.M., Barnett L., Warren C., Blackman A., Lapa e Silva J.R., Andrade B.B, & Kalams S.A. (2020). Increased Frequency of Memory CD4+ T-Cell Responses in Individuals With Previously Treated Extrapulmonary Tuberculosis. Frontiers in Immunology, 11, 605338.

+ Open protocol

+ Expand

Blocking Cytokines and Checkpoint Inhibitors in T-cell Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were thawed, washed and reconstituted in AIM V serum-free medium (Life Technologies, Oslo, Norway) containing 0.1% human serum albumin. After an overnight rest, cells were pulse-labelled with carboxyfluorescein diacetate succinimidyl ester (CFSE, Life Technologies) at a concentration of 2 μM for 5 minutes. The following blocking antibodies were added to parallel culture wells at a final concentration of 10 μg/mL: anti-IL-10 (R&D Systems, MN, USA; clone 23738), anti-TGF-β (R&D; clone 1D11), anti-PD-L1 (eBioscience, CA, USA; clone MIH1), and anti-HVEM (R&D; clone 94801).
After a 30 minute incubation, cultures were stimulated with either Gag or Env 15-mer overlapping peptide panels (NIH AIDS Research and Reference Reagent Program, MD, USA) at a final concentration of 2 μg/mL/peptide. Staphylococcal Enterotoxin B (SEB, Sigma-Aldrich, MO, USA) at a final concentration of 0.5 μg/mL was used as a positive control.
Cells were cultured at 37°C in 5% CO₂ for 5 days, harvested, and stained with the following fluorochrome-conjugated antibodies: CD3 V450, CD8 APC-H7, HLA-DR BV605, CD45RA APC (all BD) and CD25 PE (Biolegend). 7-aminoactinomycin D (7-AAD, BD) was added for dead cell exclusion. Flow cytometry data were acquired on a BD FACS Canto II with BD Diva 6.1 software, and analyzed in FlowJo X (FlowJo LLC, OR, USA).

Prebensen C., Lind A., Dyrhol-Riise A.M, & Kvale D. (2016). Regulation of Gag- and Env-Specific CD8+ T Cell Responses in ART-Naïve HIV-Infected Patients: Potential Implications for Individualized Immunotherapy. PLoS ONE, 11(4), e0153849.

+ Open protocol

+ Expand

Multiparametric Flow Cytometry Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were surface stained, fixed, and refrigerated at 4°C overnight, followed by flow cytometry using the BD LSRII instrument according to manufacturer’s protocols (BD Biosciences, Carlsbad, CA, USA). An 11-color flow panel of cross-reactive antibodies was used: CD3 Alexa700, CD4 BD-V500, CD8 BD-V450, CD20 FITC, CCR5 PE, CXCR4 PE-CF594, CCR7 PE-Cy7, CD45RA PE-Cy5, CD14 APC, CD69 APC-H7, and HLA-DR BV605 (BD Biosciences). Samples were analyzed using FlowJo (TreeStar Inc, Ashland, OR, USA), and results graphed using GraphPad Prism 5 (Graphpad Software Inc, La Jolla, CA, USA).

Pereira L.E., Makarova N., Dobard C., Aubert R.D., Srinivasan P., McNicholl J, & Smith J.M. (2014). Development and optimization of a non-enzymatic method of leukocyte isolation from macaque tissues. Journal of medical primatology, 43(5), 360-363.

+ Open protocol

+ Expand

Multiparametric Flow Cytometry of T-Cell Subsets

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were first stained with CD3-FITC, CD4-PE, CD8-APC-Cy7, CD45RA PE-Cy7, CD45RO-APC, CCR7-BV510, HLA-DR-BV605, and TCRγδ-BV421 (BD Biosciences). For intracellular staining with IFNγ-PE-Cy7, TNF-AL700, IL-2-BV421, and IL-17-BV510, cells were fixed and permeabilized using the BD Biosciences Cytofix/Cytoperm Kit. Data were acquired on BD LSRFortessa® (50,000 events), and cell frequencies, as well as median fluorescence intensity (MFI), were measured using FlowJo 10 software (Tree Star Inc.). Supernatants of PBMC cultures were collected and stored at -20°C for cytokine assays. Concentrations of IFNγ and IL-10 were measured by ELISA (R&D Systems) according to the manufacturer’s instructions.

Sarno A., Leite A., Augusto C., Muller I., de Ângelis L., Pimentel L., Queiroz A, & Arruda S. (2024). Impaired macrophage and memory T-cell responses to Bacillus Calmette-Guerin nonpolar lipid extract. Frontiers in Immunology, 14, 1263352.

+ Open protocol

+ Expand

Analyzing Cytokine Secretion in EV-Treated PBMCs

Check if the same lab product or an alternative is used in the 5 most similar protocols

PBMC were cultured in RPMI supplemented with 10% FBS, 1% antibiotic antimycotic solution and 1% glutamine (Merck) for 16h in the presence of BD GolgiPlug™ Protein Transport Inhibitor (BD Biosciences). When indicated, PBMC were treated with HuCCT-1-derived EVs at a PBMC:EVs ratio of 1:50 for 16 h. After incubation, 5 × 10⁵ PBMC were harvested and stained with anti-human CD14 BB700, CD3 BV421, HLA-DR BV605 and CD16 APC-H7 mAbs (all from BD Biosciences) for 30 min at 4 °C. Cells were subsequently fixed with BD Cytofix/Cytoperm (BD Biosciences) and permeabilized with the BD Perm/Wash buffer (BD Biosciences) in the presence of anti-human MIP-1α FITC, IL-1α PE and IL-8 BV510 mAbs according to the manufacturer’s instructions. FACSCelesta (BD Biosciences) instrument and Kaluza™ software (Beckman Coulter) were used for data acquisition and analysis.

Oliviero B., Dei Cas M., Zulueta A., Maiello R., Villa A., Martinelli C., Del Favero E., Falleni M., Montavoci L., Varchetta S., Mele D., Donadon M., Soldani C., Franceschini B., Maestri M., Piccolo G., Barabino M., Bianchi P.P., Banales J.M., Mantovani S., Mondelli M.U, & Caretti A. (2023). Ceramide present in cholangiocarcinoma-derived extracellular vesicle induces a pro-inflammatory state in monocytes. Scientific Reports, 13, 7766.

+ Open protocol

+ Expand

Profiling Antigen-Presenting Cells in Murine Spleen

Check if the same lab product or an alternative is used in the 5 most similar protocols

Spleens were finely minced and passed through a 70-μM nylon cell strainer (Becton Dickinson, Franklin Lakes, NJ). Spleen cells were collected in RPMI 1640 (Life Technologies, Paisley, Scotland) supplemented with 10% preselected fetal calf serum (FCS), 2 mM l-glutamine (both from Hyclone, Logan, UT), 100 U/mL penicillin, 100 mg/mL streptomycin (both from Life Technologies), and 5 × 10–5 M β-mercaptoethanol (Sigma-Aldrich). Single-cell suspensions were cleared of erythrocytes by hemolysis using a hypotonic buffer (pH 7.2) composed of 0.15 M ammonium chloride, 10 mM potassium bicarbonate (both from Merck, Darmstadt, Germany), and 0.1 mM ethylene-diaminetetraacetic acid (Life Technologies).
To evaluate the composition of APCs, splenocytes were stained for CD3e PerCP-eFluor 710 (clone 17A2, Thermo Fisher Scientific), CD19 FITC (clone 1D3, BD Biosciences), CD11c APC (HL3, BD Biosciences), CD11b PE-Cy7 (clone M1/70, Thermo Fisher Scientific) and HLA-DR BV605 (clone G46-6, BD Biosciences) and analyzed using a BD FACS Aria III (BD Biosciences) and FlowJo software (Version 10.8.1; BD Biosciences). Nonspecific binding through Fc gamma receptors was blocked by a mixture of anti-CD16 and anti-CD32 antibodies (Fc Block; BD Biosciences).

Lubich C., Steinitz K.N., Hoelbl B., Prenninger T., van Helden P.M., Weiller M, & Reipert B.M. (2022). Modulating the microenvironment during FVIII uptake influences the nature of FVIII-peptides presented by antigen-presenting cells. Frontiers in Immunology, 13, 975680.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!