Fl 1321 2

Manufactured by Vector Laboratories

Sourced in United States

The FL-1321-2 is a fluorescence detection instrument designed for laboratory use. It is capable of measuring fluorescence intensity.

Automatically generated - may contain errors

Lab products found in correlation

Ab246887, by Abcam (2 mentions) Ab26350, by Abcam (2 mentions) Ab150114, by Abcam (2 mentions) Ab150077, by Abcam (2 mentions) Ab185703, by Abcam (1 mentions) A21207, by Thermo Fisher Scientific (1 mentions) Alexa fluor 594 dye, by Thermo Fisher Scientific (1 mentions) A1r storm, by Nikon (1 mentions) Anti 8 ohdg antibody, by Santa Cruz Biotechnology (1 mentions) Fl 1031, by Vector Laboratories (1 mentions) Alexa fluor 488 labeled, by Thermo Fisher Scientific (1 mentions) Ab110641, by Abcam (1 mentions) Ab209813, by Abcam (1 mentions) Ab210471, by Abcam (1 mentions) Fl 1111 2, by Vector Laboratories (1 mentions) Wt1 sc 192, by Santa Cruz Biotechnology (1 mentions) Ab3553, by Merck Group (1 mentions) Ab92547, by Abcam (1 mentions) Ab15580, by Abcam (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions)

5 protocols using fl 1321 2

Kidney Oxidative Stress and Co-Localization

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After dewaxing, rehydration, and blocking with BSA, the kidney cortex sections were treated with anti-8-OHdG antibody (Santa Cruz company, sc-66036) overnight at 4 °C. Then, the Alexa Fluor 488–labeled secondary antibody (A-11001, Thermo Fisher Scientific) was used. For detection of co-localization of Tim-3 and LTL/DBA, the primary antibodies were anti-Tim-3 antibody (1:100, ab185703, Abcam), anti-LTL antibody (1:200, FL-1321-2, Vector Laboratories), and anti-DBA antibody (1:100, FL-1031, Vector Laboratories), the secondary antibody was IgG antibody conjugated with Alexa Fluor 594 dye (1:1000, A21207, Thermo Fisher Scientific). After DAPI staining of nuclei, the sections were photographed under a fluorescence microscope (Nikon A1R Storm).

Li P., Li X., Wu W., Hou M., Yin G., Wang Z., Du Z., Ma Y., Lou Q, & Wei Y. (2023). Tim-3 protects against cisplatin nephrotoxicity by inhibiting NF-κB-mediated inflammation. Cell Death Discovery, 9, 218.

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Immunohistochemical Profiling of Cellular Markers

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Lectin: PHA-L (Phaseolus vulgaris leucoagglutinin lectin) and LTL (Lotus Tetragonolobus lectin) (FL-1111-2 and FL-1321-2, Vector Laboratories). Primary antibodies: anti-Smarca4/Brg1 (ab110641, Abcam), -Wt1 (sc192, Santa Cruz Biotechnology); -THP (Santa Cruz, sc-271022), -NCC (EMD Millipore, AB3553), –phosphohistone-H3 (EMD Millipore, 06-570), -Tnnt2 (ab209813, Abcam), -aSMA (clone 1A4 and A5228, Sigma), -Ncam (sc-1507, Santa Cruz), -Vimentin (ab92547, Abcam), -Pttg1 (HPA045034, Atlas antibodies), -Tyrobp (MBS7127061, MyBiosource), -Foxd1 (MBS9204091, MyBiosource), and -Dlk1 (ab210471, Abcam).

Xu J., Zhou X., Zhang T., Zhang B, & Xu P.X. (2023). Smarca4 deficiency induces Pttg1 oncogene upregulation and hyperproliferation of tubular and interstitial cells during kidney development. Frontiers in Cell and Developmental Biology, 11, 1233317.

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Immunofluorescence Analysis of Kidney Sections

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Immunofluorescence analysis was performed on kidney sections (2-μm thickness) and HK-2 cells. Slides were incubated with antibodies against anti-γH2AX (ab26350, Abcam), anti-CDK12 (ab246887, Abcam), anti-Ki-67 (ab15580, Abcam), anti-PCNA (ab29, Abcam), and LTL (FL-1321-2, Vector Laboratories) and incubated with secondary antibodies (ab150114 and ab150077, Abcam). Nuclei were stained with DAPI (Sigma-Aldrich) according to the manufacturer's instructions. Under a confocal microscope (FV3000, Olympus), 10 fields of view were randomly assigned, and the number of CDK12-positive cells was counted in a blinded manner.

Zhang Y.L., Tang T.T., Ni W.J., Li Z.T., Jiang L.Y., Wang Y., Zhou X., Cao J.Y., Yin Q., Jiang W., Zhao Y.J., Gan W.H., Zhang A.Q., Li Z.L., Wen Y., Lv L.L., Liu B.C, & Wang B. (2024). Tubule-specific cyclin-dependent kinase 12 knockdown potentiates kidney injury through transcriptional elongation defects. International Journal of Biological Sciences, 20(5), 1669-1687.

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Immunofluorescence Analysis of Renal Tissues

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Immunofluorescence analysis was performed on 4 μm thick renal tissue sections and HK-2 cells. They were performed with anti-TRF1 (ab1423, Abcam), anti-γH2AX (ab26350), anti-CDK12 (ab246887, Abcam) , AQP1 (ab 9566, Abcam) , LTL (FL-1321-2, Vector Laboratories) and incubated with secondary antibodies (ab150114 and ab150077, Abcam). Under the confocal microscope, 10 fields of view were randomly assigned, and the number of positive tubules and positive cells was counted in a blind manner.

Yin Q., Zhao Y.J., Ni W.J., Tang T.T., Wang Y., Cao J.Y., Yin D., Wen Y., Li Z.L., Zhang Y.L., Jiang W., Zhang Y., Lu X.Y., Zhang A.Q., Gan W.H., Lv L.L., Liu B.C, & Wang B. (2022). MiR-155 deficiency protects renal tubular epithelial cells from telomeric and genomic DNA damage in cisplatin-induced acute kidney injury. Theranostics, 12(10), 4753-4766.

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Protein Expression in Kidney Proximal Tubules

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We conducted IF staining on frozen kidney sections to examine protein expression in proximal tubules (n = 5). The sections were incubated at 4°C overnight with primary antibodies, including NDRG2 (1:300, #5667, Cell Signaling Technology, Boston, USA), light chain 3 (LC3, 1:400, #ab192890, Abcam, Cambridge, United Kingdom), P62 (1:400; #ab56416, Abcam), and Parkin (1:100, #66674-1-lg, Proteintech, Chicago, USA). The sections were then incubated with secondary antibodies for 2 h at room temperature in the dark. To identify proximal tubules, we performed lotus tetragonolobus lectin (LTL) staining by adding annexin V-fluorescein isothiocyanate-conjugated LTL (1:100, #FL-1321-2, Vector Laboratories, San Francisco, USA) during secondary antibody incubation. Finally, the sections were fixed with 50% glycerol and imaged by fluorescence microscopy.

Liu M., Chen J., Sun M., Zhang L., Yu Y., Mi W., Ma Y, & Wang G. (2024). Protection of Ndrg2 deficiency on renal ischemia-reperfusion injury via activating PINK1/Parkin-mediated mitophagy. Chinese medical journal.

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