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Micropet r4 scanner

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The MicroPET R4 scanner is a small-animal positron emission tomography (PET) imaging system designed for preclinical research. It provides high-resolution, quantitative imaging of small animals such as mice and rats. The MicroPET R4 scanner is capable of detecting and measuring the distribution of positron-emitting radioactive tracers within the subject being imaged.

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Lab products found in correlation

Inveon spect ct scanner, by Siemens (4 mentions) Inveon micro ct scanner, by Siemens (1 mentions)

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MicroPET R4 rodent model scanner MicroPET R4 rodent scanner R4 microPET Siemens scanners

10 protocols using micropet r4 scanner

1

Multimodal Imaging of Rat Biodistribution

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All studies involving animals were performed under a protocol (A 6-13-13) approved by the Institutional Animal Care and Use Committee of Wayne State University. Sprague-Dawley rats (200–250 g, N = 3) were anesthetized with 3% isoflurane in oxygen and maintained at 2% isoflurane in oxygen throughout the imaging studies. The body temperature was maintained using electronically-controlled heating pad (M2M Imaging, Cleveland, OH) set at 37°C. Anesthetized rats were placed in the microPET R4 scanner (Siemens, Knoxville, TN) in the supine position with the long axis of the animal parallel to the long axis of the scanner with the brain positioned in the center of the field of view. Each radiotracer (300–500 μCi/animal) was administered in saline via the tail-vein injection in a total volume ≤1.25 ml. Dynamic PET images were obtained over 60 minutes, followed by 2 overlapping frames (5 min each) acquired to obtain a whole body images of radiotracer biodistribution in other organs and tissues. After PET imaging, the positioning bed with the affixed anesthetized animal was transferred to the Inveon SPECT/CT scanner (Siemens, Knoxville, TN) and CT images and 4 overlapping frames (2 min each) were acquired covering the whole body using X-ray tube settings of 80 kV and 500 uA.
Bonomi R., Mukhopadhyay U., Shavrin A., Yeh H.H., Majhi A., Dewage S.W., Najjar A., Lu X., Cisneros G.A., Tong W.P., Alauddin M.M., Liu R.S., Mangner T.J., Turkman N, & Gelovani J.G. (2015). Novel Histone Deacetylase Class IIa Selective Substrate Radiotracers for PET Imaging of Epigenetic Regulation in the Brain. PLoS ONE, 10(8), e0133512.
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2

Multimodal Imaging of Sprague-Dawley Rats

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Sprague−Dawley rats (200−250 g, n = 3) were anesthetized with 3% isoflurane in oxygen and maintained at 2% isoflurane in oxygen throughout the imaging studies. The body temperature was maintained using electronically controlled heating pad (M2M Imaging, Cleveland, OH) set at 37°C. Anesthetized rats were placed in a stereotactic head holder made of polycarbonate plastic (Kopf, Tujunga, CA) and attached to the bed of the microPET R4 scanner (Siemens, Knoxville, TN) in the supine position with the long axis of the animal parallel to the long axis of the scanner and the brain positioned in the center of the FOV. Each radiotracer (300−500 μCi/animal) was administered in saline via the tail vein in a total volume ≤1.25 mL as a slow bolus injection over a period of 1 min. Dynamic PET images were obtained over 60 min. After PET imaging, the positioning bed with the affixed anesthetized animal was transferred to an Inveon SPECT/CT scanner (Siemens, Knoxville, TN) and CT images and four overlapping frames (2 min each) were acquired covering the whole body using X-ray-tube settings of 80 kV and 500 μA with an exposure time of 300−350 ms for each of the 360 rotational steps.
Bonomi R., Popov V., Laws M.T., Gelovani D., Majhi A., Shavrin A., Lu X., Muzik O., Turkman N., Liu R., Mangner T, & Gelovani J.G. (2018). Molecular Imaging of Sirtuin1 Expression−Activity in Rat Brain Using Positron-Emission Tomography−Magnetic-Resonance Imaging with [18F]-2-Fluorobenzoylaminohexanoicanilide. Journal of medicinal chemistry, 61(16), 7116-7130.
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3

Biodistribution of 64Cu-DOTA-FA-FI-G5 Dendrimers

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About 7.4 MBq of 64Cu-DOTA-FA-FI-G5·NHAc dendrimers was intravenously injected into each mouse (n = 6 per group) under isoflurane anesthesia. Five-minute static scans were acquired at 1, 2, 4, and 6 h post-injection (pi), and a ten-minute static scan was acquired at 20 h pi. The images were reconstructed by a two dimensional (2D) ordered-subsets expectation maximum (OSEM) algorithm. The microPET scans and imaging analysis were performed using a rodent scanner (microPET R4 scanner; Siemens Medical Solutions USA, Inc., Knoxville, TN). For each microPET scan, the regions of interest were drawn over the tumor, the normal tissues, and the major organs on the decay-corrected whole-body coronal images. The radioactivity concentration (accumulation) within the tumor, muscle, liver, and kidneys was obtained from the mean value within the multiple regions of interest and then converted to %ID g-1. For ex vivo microPET imaging, the mice were euthanized and dissected at 20 h pi of the 64Cu-DOTA-FA-FI-G5·NHAc dendrimers (7.4 MBq). The blood, the tumor, the major organs, and the tissues were collected, and a ten-minute static microPET scan was acquired. For the blocking experiment, mice bearing KB tumors were scanned after the co-injection of 64Cu-DOTA-FA-FI-G5·NHAc dendrimers (7.4 MBq) with 100 μg of free FA per animal.
Ma W., Fu F., Zhu J., Huang R., Zhu Y., Liu Z., Wang J., Conti P.S., Shi X, & Chen K. (2018). 64Cu-Labeled multifunctional dendrimers for targeted tumor PET imaging. Nanoscale, 10(13), 6113-6124.
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4

Radiosynthesis and Administration of [18F]TFAHA

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The radiosynthesis and formulation of [18F]TFAHA for intravenous (i.v.) injection was performed as previously described42 (link). Under inhalation anesthesia (as described above), the rat head fixed in a stereotactic holder made of polycarbonate plastic (Kopf-Tujunga, Germany), attached to the bed of microPET R4 scanner (Siemens, Knoxville, TN), and the head was positioned in the center of the field of view (FOV). Then, the rat was injected via the tail vein with [18F]TFAHA (350–500 µCi in 1 ml), as a steady injection over 1 min; PET images were obtained in a dynamic mode over 30 minutes post radiotracer administration. Thereafter, the detachable bed with the affixed animal was transferred into the Inveon SPECT/CT scanner (Siemens, Knoxville, TN) and CT images of the head were acquired using 80 kV and 500 uA current settings with exposure time of 300–350 milliseconds at each of the 360 rotational steps.
Laws M.T., Bonomi R.E., Kamal S., Gelovani D.J., Llaniguez J., Potukutchi S., Lu X., Mangner T, & Gelovani J.G. (2019). Molecular imaging HDACs class IIa expression-activity and pharmacologic inhibition in intracerebral glioma models in rats using PET/CT/(MRI) with [18F]TFAHA. Scientific Reports, 9, 3595.
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5

Multimodal Imaging of Brain PET/CT

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Baseline of 2-[18F]BzAHA PET/CT studies was performed a day after the MRI studies. The radiosynthesis and formulation of 2-[18F]BzAHA for intravenous (i.v.) injection was performed as previously described45 (link); under inhalation anesthesia (as described above). Anesthetized rats were placed in a stereotactic head holder made of polycarbonate plastic (Kopf-Tujunga) and attached to the bed the microPET R4 scanner (Siemens) in the supine position with the long axis of the animal parallel to the long axis of the scanner and the brain positioned in the center of the FOV. The radiotracer (300–500 µCi/animal) was administered in saline via the tail vein in a total volume of ≤1.25 mL, as a slow bolus injection over the period of 1 min. Dynamic PET images were obtained over 60 min. After PET imaging, the positioning bed with the affixed anesthetized animal was transferred to the Inveon SPECT/CT scanner (Siemens) and CT images were acquired in 4 overlapping frames (2 min each) covering the whole body using X-ray tube settings of 80 kV and 500 µA with exposure time of 300–350 ms of each of the 360 rotational steps.
Laws M.T., Bonomi R.E., Gelovani D.J., Llaniguez J., Lu X., Mangner T, & Gelovani J.G. (2020). Noninvasive quantification of SIRT1 expression–activity and pharmacologic inhibition in a rat model of intracerebral glioma using 2-[18F]BzAHA PET/CT/MRI. Neuro-oncology Advances, 2(1), vdaa006.
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6

Microimaging Glucose Metabolism in Mice

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MicroPET imaging was performed as described before10 (link) at the Molecular Imaging Center at the Department of Radiology, University of Southern California, with guidance from Dr. Li-Peng Yap. Blood-glucose levels were measured before the administration of the tracer to ensure that changes in glucose metabolism during [18F]- FDG-PET imaging were not due to differences in starting blood glucose levels, but represented intrinsic activity of the brain. After an overnight fasting period on drinking-water only, both lipoic acid-treated and control groups were sedated using 2% isoflurane by inhalation and administered the radiotracer 2-deoxy-2 [18F]-fluoro-D-glucose intravenously, followed by imaging in the Siemens MicroPET R4 scanner for 60 min. The mice, still sedated, were immediately transferred to the Siemens Inveon microCT scanner for 5 min for a CT scan. AMIDE (Free Software Foundation, Inc., Boston, MA, USA) was used to define region of interest and Standard uptake values (SUVs) was calculated based on the time, dose, and weight of the animal.
Liu Z., Patil I., Sancheti H., Yin F, & Cadenas E. (2017). Effects of Lipoic Acid on High-Fat Diet-Induced Alteration of Synaptic Plasticity and Brain Glucose Metabolism: A PET/CT and 13C-NMR Study. Scientific Reports, 7, 5391.
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7

Quantifying Tumor Metabolism via microPET Imaging

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MicroPET is a tomography imaging device specially used for in vivo experimental research on small animals. By injecting the imaging agent 18F-fludeoxyglucose (FDG) in vivo, microPET can detect the level of glucose metabolism in tumor tissue, to analyze the inhibition of metabolic activity in tumor, along with morphology evaluation. After the last administration, one mouse from each of the above groups was randomly selected and fasted for 12 h before microPET scanning. A dose of 150±20mCi 18F-FDG was injected into nude mice via tail vein. The nude mice were allowed to move freely for about 30 minutes before anesthetized with 2% isoflurane inhalation, then fixed to microPET examination bed and kept warm during a 10 minutes scanning using microPET R4 scanner (Siemens Medical Solutions, Malvern, PA, USA). Images were obtained after data reconstruction.
Yan M., Chen J., Jiang H., Xie Y., Li C., Chen L., Yang B, & Cao J. (2020). Effective inhibition of cancer cells by recombinant adenovirus expressing EGFR-targeting artificial microRNA and reversed-caspase-3. PLoS ONE, 15(8), e0237098.
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8

Longitudinal PET Imaging of Rat Behavior

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PET scans were performed on days 1, 2 and 18 (Fig. 1). All 30 rats were fasted overnight before the PET scans. [ 18 F]FDG, which was used as the radioactive tracer for the PET imaging, was injected intraperitoneally at a dose of (0.91 ± 0.16)mCi into the rats before the behavioural training (Tian et al., 2014) (link). The rats were anesthetized with 5% iso urane, and then placed in a prone position on the bed of the scanner. 2% iso urane was used to maintain anesthesia during scanning. The data acquisition was performed using a microPET R4 scanner (Siemens Medical Solutions) for 30 minutes after a 40-minute uptake period of [ Proportional scaling was used for global normalization. The difference in the level of [ 18 F]FDG in the brain among the groups was compared, the difference in the activation signal in the brain was calculated when the rats were in different behavioural states (p (uncorrected) < 0.001), and more than 50 consecutive voxel aggregation highlights were considered signi cant differences among the groups.
Liu J., Yu J., Liu H.B., Yao Q, & Zhang Y. (2022). Chronic fluoxetine enhances extinction therapy for PTSD by evaluating brain glucose metabolism in rats: an [18F]FDG PET study. Annals of nuclear medicine, 36(12).
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9

Longitudinal PET Imaging of Rat Behavior

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PET scans were performed on days 1, 2 and 18 (Fig. 1). All 30 rats were fasted overnight before the PET scans. [ 18 F]FDG, which was used as the radioactive tracer for the PET imaging, was injected intraperitoneally at a dose of (0.91 ± 0.16)mCi into the rats before the behavioural training (Tian et al., 2014) (link). The rats were anesthetized with 5% iso urane, and then placed in a prone position on the bed of the scanner. 2% iso urane was used to maintain anesthesia during scanning. The data acquisition was performed using a microPET R4 scanner (Siemens Medical Solutions) for 30 minutes after a 40-minute uptake period of [ Proportional scaling was used for global normalization. The difference in the level of [ 18 F]FDG in the brain among the groups was compared, the difference in the activation signal in the brain was calculated when the rats were in different behavioural states (p (uncorrected) < 0.001), and more than 50 consecutive voxel aggregation highlights were considered signi cant differences among the groups.
Liu J., Yu J., Liu H.B., Yao Q., & Zhang Y. (2021). Chronic Fluoxetine Enhances Extinction Therapy for PTSD by Evaluating Brain Glucose Metabolism in Rats: An [18F]FDG PET Study.
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10

PET Imaging of Rat Behavior

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PET imaging studies were done on days 1, 2, and 5 (Fig. 1A). Each rat performed the behavior training for 30 min, and PET images were acquired in the micro-PET R4 scanner (Siemens Medical Solutions) at 40 min after intraperitoneal injection of 18 F-FDG (18.5 MBq). Images were analyzed by statistical parametric mapping (12) .
Zhu Y., Du R., Zhu Y., Shen Y., Zhang K., Chen Y., Song F., Wu S., Zhang H, & Tian M. (2016). PET Mapping of Neurofunctional Changes in a Posttraumatic Stress Disorder Model. Journal of nuclear medicine : official publication, Society of Nuclear Medicine, 57(9).
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