Methanol

The samples of isoproterenol hydrochloride obtained from different final step batches, reference standard of isoproterenol hydrochloride (purity 99.9%) and the starting material, 1-(3,4-bis(benzyloxy)phenyl)-2-(isopropylamino)ethanone hydrochloride (2) used in the synthesis, were provided by Chemical Research Division, Micro Labs Ltd. (Bangalore, India). The catalyst, Pd-C (50% wet), was acquired from Monarch Catalyst Private Limited (Mumbai, India). HPLC grade acetonitrile and methanol were purchased from Spectrochem Pvt Ltd. (Bangalore, India), and formic acid and trifluoroacetic acid were purchased from Sigma-Aldrich (Bangalore, India). The commercial grade ethyl acetate (EtOAc) and isopropyl alcohol (IPA) were procured from Taiwan Fieldrich Corporation (Taipei, China). Purified water by Millipore Milli-Q Plus Purification System (Bradford, PA, USA) was used during the course of experimental studies. CD₃OD was purchased from Cambridge Isotope Laboratories, Inc. (Andover, MA, USA). Potassium bromide used in Fourier transform infrared spectroscopy (FT-IR) was purchased from Merck KGaA (Darmstadt, Germany). TLC plates (60 F₂₅₄) procured from Merck (Delhi, India) was used to check the progress of reaction.

Cesium
carbonate (Cs₂CO₃), lead bromide (PbBr₂), 1-octadecene(ODE), oleic acid
(OA), and oleylamine (OLAM) were purchased from Sigma-Aldrich and
used without any further purification. Tetrachloroauric acid (HAuCl₄·3H₂O) was purchased from Sigma-Aldrich. Sodium
borohydride (NaBH₄), sodium hydroxide (NaOH), tetraoctyl
ammonium bromide (TOABr), and 2-phenylethanethiol (PhC₂H₄SH, 99%) were purchased from Merck India Ltd. Methyl
acetate (MeOAC, Merck) was used during the purification of the nanocrystals.
Solvents like toluene, ethanol, methanol, acetonitrile, and dichloromethane
were obtained from Spectrochem. toluene (Spectrochem) was used for
all spectroscopic experiments, including the characterization of the
nanocrystals.

All of the chemicals were utilized without
additional purification. Y(NO₃)₃·6H₂O (99.99%), Yb(NO₃)₃·6H₂O (99.99%), Er(NO₃)₃·6H₂O (99.99%),
diethylene glycol, poly(acrylic acid) (PAA), and ethylenediamine (reagent,
99%) were purchased from Sigma-Aldrich. Oleic acid (90%, technical
grade), 1-octadecene (90%, technical grade), and hydrochloric acid
(HCl, analytical reagent 35–38%) were purchased from Alfa Aesar.
Rhodamine-B (RhB, 95%) and hydrazine hydrate 80 wt% solution in H₂O were purchased from Loba Chemie Pvt. Ltd. Ammonium fluoride
(NH₄F, 99%), sodium hydroxide (NaOH, 97%), cyclohexane
(C₆H₁₂ 99%), ethanol (C₂H₅OH, analytical reagent 99%), and methanol (CH₄OH, analytical
reagent 99%) were purchased from Spectrochem Pvt. Ltd. Double distilled
water was utilized to prepare all of the aqueous solutions. Aqueous
solutions of Sn²⁺, Hg²⁺, Co²⁺, Mg²⁺, Ni²⁺, Ca²⁺, Zn²⁺, Co²⁺, Cd²⁺, Na⁺, K⁺, and Cs⁺ were made from the corresponding halide salts.

Domesticated B. mori silk cocoons were obtained from a local farmer
based in Palakkad, Kerala, India. Lithium bromide, sodium carbonate,
formic acid (≥96%), sodium azide, dialysis bag (molecular weight
cut-off, 12–14 kDa), sodium hydroxide, paraformaldehyde, and
protease (type XIV from Streptomyces griseus, ≥3.5 units/mg) were purchased from Sigma-Aldrich, India.
1,1,1,3,3,3-Hexafluoro-2-isopropanol (98%) and methanol were purchased
from Spectrochem, India. Dulbecco’s modified Eagle’s
medium (DMEM), fetal bovine serum, penicillin–streptomycin,
trypsin, Alamar blue, Hoechst, and rhodamine-phalloidin were bought
from Thermo Fisher, India. The MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium)
reagent and phosphate-buffered saline (PBS) powder were obtained from
Hi-Media, India. L929 (mouse subcutaneous connective fibroblasts)
cells were purchased from American Type Culture Collection, USA, and
SIRC (Statens Serum Institut Rabbit Cornea) cells were obtained from
National Centre for Cell Science, Pune, India.