Imalgene 1000

Manufactured by AstraZeneca

Sourced in Germany

Imalgene 1000 is a high-performance laboratory equipment used for the analysis and detection of various biological and chemical compounds. It is a versatile instrument that employs advanced imaging and detection technologies to provide accurate and reliable results. The core function of Imalgene 1000 is to facilitate the analysis and quantification of samples, enabling researchers and scientists to gain valuable insights into their investigations.

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Lab products found in correlation

Sodium pentobarbital, by Sanofi (3 mentions) Omnican, by B. Braun (3 mentions) Rompun 2, by Bayer (2 mentions) Rompun, by Bayer (1 mentions)

3 protocols using imalgene 1000

Anesthesia and Euthanasia Protocol for Rats

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30 min after coming out of the hyperbaric chamber, all the animals were anesthetized by induction with isoflurane (Bellamont, firstly at 5% then 2%), then by intraperitoneal injection (1 ml syringe, Omnican, B. Braun, Melsungen, Germany) of a mixture of ketamine (Imalgene 1000, 100 mg/kg, AstraZeneca, London, UK), acepromazine (Calmivet, 1,65 mg/kg, Vétoquinol S.A., Lure, France) and xylazine (Rompun 2%, 16 mg/kg, Bayer HealthCare, KVP, Kiel, Germany).
At the end of the experiment, rats were sacrificed by an injection of sodium pentobarbital (200 mg/kg IP; Sanofi, Paris, France).

de Maistre S., Gaillard S., Martin J.C., Richard S., Boussuges A., Rives S., Desruelle A.V., Blatteau J.E., Tardivel C., Risso J.J, & Vallée N. (2020). Cecal metabolome fingerprint in a rat model of decompression sickness with neurological disorders. Scientific Reports, 10, 15996.

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Anesthesia and Euthanasia Protocol for Rats

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Coming out of the hyperbaric chamber after 60 min, all the animals were anesthetized by induction with isoflurane (Bellamont, first at 5% and then at 2%), then by intraperitoneal injection (1-ml syringe, Omnican^®, B. Braun, Melsungen, Germany) of a mixture of ketamine (Imalgene 1000, 100 mg/kg, AstraZeneca, London, United Kingdom), acepromazine (Calmivet^®, 1.65 mg/kg, Vétoquinol S.A., Lure, France) and xylazine (Rompun^® 2%, 16 mg/kg, Bayer HealthCare, KVP, Kiel, Germany).
At the end of the experiment, rats were sacrificed by an injection of sodium pentobarbital (200 mg/Kg IP; Sanofi, Paris, France).

Lambrechts K., de Maistre S., Abraini J.H., Blatteau J.E., Risso J.J, & Vallée N. (2018). Tirofiban, a Glycoprotein IIb/IIIa Antagonist, Has a Protective Effect on Decompression Sickness in Rats: Is the Crosstalk Between Platelet and Leukocytes Essential?. Frontiers in Physiology, 9, 906.

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Anesthesia and Euthanasia Protocol for Rats

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Thirtyminutes after the physical examination, the animals are anesthetized by induction with isoflurane (Bellamont, firstly at 5% then 2% in 100% air flow at 2.0 L/min), then by intraperitoneal injection (1 ml syringe, Omnican, B. Braun, Melsungen, Germany) of a mixture of ketamine (Imalgene 1000, 100 mg/kg, AstraZeneca, London, UK), acepromazine (Calmivet, 1.65 mg/kg, Vétoquinol S.A., Lure, France) and xylazine (Rompun 2%, 16 mg/kg, Bayer HealthCare, KVP, Kiel, Germany). Anaesthesia level is determined by testing the lack of withdrawal reflexes in response to pinches of the distal hind limbs. At the end of the experiment, rats are sacrificed by an injection of sodium pentobarbital (200 mg/kg IP; Sanofi, Paris, France).

Desruelle A.V., de Maistre S., Gaillard S., Richard S., Tardivel C., Martin J.C., Blatteau J.E., Boussuges A., Rives S., Risso J.J, & Vallee N. (2022). Cecal Metabolomic Fingerprint of Unscathed Rats: Does It Reflect the Good Response to a Provocative Decompression?. Frontiers in Physiology, 13, 882944.

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