Cytidine monophosphate

Manufactured by Merck Group

Sourced in United States

Cytidine monophosphate is a nucleotide used in biochemical research and laboratory applications. It is a mononucleotide composed of cytosine, ribose, and a single phosphate group. Cytidine monophosphate serves as a fundamental building block for various biomolecular processes.

Automatically generated - may contain errors

Lab products found in correlation

Close spelling variants of this product

Cytidine (56 citations) Cytidine 5′-monophosphate (11 citations) Cytidine 5′-monophosphate disodium salt (6 citations) Cytidine 5′‐monophosphate (5′‐CMP) (2 citations) Disodium cytidine 5′-monophosphate (2 citations)

5 protocols using cytidine monophosphate

Enzymatic DNA Nucleotide Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Calf thymus (CT) DNA was purchased from Rockland Immunochemicals (Pottstown, PA, USA). Deoxyadenosine monophosphate (dAMP), thymidine monophosphate (TMP), deoxycytidine monophosphate (dCMP), deoxy guanosine monophosphate (dGMP), adenosine monophosphate (AMP), uridine monophosphate (UMP), cytidine monophosphate (CMP), guanosine monophosphate (GMP), nuclease P1 and deoxyribonuclease I (DNase I) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Inosine monophosphate (IMP) was purchased from Cayman Chemical (Ann Arbor, MI, USA). DNeasy Blood & Tissue Kit was purchased from Qiagen (Valencia, CA, USA). Acetonitrile and water (MS grade) were purchased from EMD (Gibbstown, NJ, USA).

Ma Y., Chen B, & Zhang D. (2020). Quantitation of DNA by nuclease P1 digestion and UPLC-MS/MS to assess binding efficiency of pyrrolobenzodiazepine. Journal of Pharmaceutical Analysis, 10(3), 247-252.

+ Open protocol

+ Expand

Screening for Small Molecule Inhibitors

Check if the same lab product or an alternative is used in the 5 most similar protocols

Structural data for the compounds available in the chemical library were provided by Namiki Shoji (Tokyo, Japan). Compounds selected by in silico SCR were purchased from Namiki Shoji. For the control compounds, adenosine monophosphate (AMP), uridine monophosphate (UMP), cytidine monophosphate (CMP), guanine monophosphate (GMP), and adenosine were purchased from Sigma-Aldrich (St. Louis, MO, USA). BCI-137 was purchased from Enamine (Kyiv, Ukraine).

Harumoto T., Sato A., Takayama Y., Miyagi H., Saito J.I, & Shinohara F. (2020). Structure-guided screening strategy combining surface plasmon resonance with nuclear magnetic resonance for identification of small-molecule Argonaute 2 inhibitors. PLoS ONE, 15(7), e0236710.

+ Open protocol

+ Expand

Quantification of Nucleoside Analog Triphosphates

Check if the same lab product or an alternative is used in the 5 most similar protocols

3TC-TP, CBV-TP, CBV, ¹⁵N₂¹³C-3TC, d₄-ABC, and emtricitabine-TP (FTC-TP) were obtained from Toronto Research Chemicals (North York, Canada). 3TC and ABC were provided by GlaxoSmithKline Inc. (Research Triangle Park, NC). Type XA acid phosphatase (EC 3.1.3.2) from sweet potatoes, guanosine, guanosine monophosphate (GMP), guanosine diphosphate (GDP), guanosine triphosphate (GTP), cytidine, cytidine monophosphate (CMP), cytidine diphosphate (CDP), and cytidine triphosphate (CTP) were purchased from Sigma Chemical Co. (St. Louis, MO). Sep-Pak QMA anion-exchange cartridges and OASIS HLB reverse-phase cartridges were purchased from Waters Corp. (Milford, MA). Potassium chloride (KCl) was purchased from JT Baker Co. (Phillipsburg, NJ). LC-MS grade (Optima) water (H₂O), acetonitrile (ACN), methanol (MeOH), acetic acid, ammonium hydroxide, and ammonium bicarbonate were purchased from Fisher chemicals (Fair Lawn, NJ). RPMI media 1640, phosphate buffered saline (PBS), Hanks’ Balanced salt solution (HBSS), and fetal bovine serum were purchased from Invitrogen (Gibco; Carlsbad, CA).

Gautam N., Lin Z., Banoub M.G., Smith N.A., Maayah A., McMillan J., Gendelman H.E, & Alnouti Y. (2018). Simultaneous Quantification of Intracellular Lamivudine and Abacavir Triphosphate Metabolites by LC-MS/MS. Journal of pharmaceutical and biomedical analysis, 153, 248-259.

+ Open protocol

+ Expand

Quantification of NNK and Metabolites in Zebrafish

Check if the same lab product or an alternative is used in the 5 most similar protocols

4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) was obtained from LGC-Dr Ehrenstorfer (LGC Standards, Barcelona, Spain) and 4-Hydroxy-4-(3-pyridyl)-butyric acid (HPBA) was purchased from Toronto Research Chemicals (TRC, Ontario, Canada). The metabolites hypoxanthine, cytidine monophosphate (CMP), guanosine monophosphate (GMP), S-adenosylmethionine (SAM), and uridine monophosphate (UMP) were obtained from Sigma-Aldrich (Sigma-Aldrich, St. Louis, MO, USA). Internal standard (IS) solution was prepared with 1 mg mL -1 of succinic-d4 acid and myristic-d27 acid in methanol also from Sigma-Aldrich. All solvents used in the preparation of solutions were LC-MS grade. Fish water was prepared with reverse-osmosis purified water containing 90 µg mL -1 of Instant Ocean ® , 0.58 mM CaSO4•2H2O (Aquarium Systems, Sarrebourg, France).

Merino C., Casado M., Piña B., Vinaixa M., & Ramírez N. (2021). Toxicity of 4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone (NKK) in early development: a wide-scope metabolomics assay in zebrafish embryos.

+ Open protocol

+ Expand

Crystalline Amino Acids in Krill Hydrolysate Diets

Check if the same lab product or an alternative is used in the 5 most similar protocols

Crystalline amino acids arginine (Arg), alanine (Ala), proline (Pro), leucine (Leu), phenylalanine (Phe) and nucleotides (AMP, guanosine monophosphate (GMP), cytidine monophosphate (CMP), inosine monophosphate (IMP)) were purchased from Sigma Aldrich (Oslo, Norway) and used singly or mixed in combination at levels similar to those in krill hydrolysate (KH) (Kousoulaki et al., 2013) :
A5) Arg+Ala+Pro+Leu+Phe + nu + rest FAA as in KH Each mix was added to one of five otherwise identical low fishmeal (3%) diets. A 15% fishmeal (MFM) diet and a 3% fishmeal diet (LFM) served as positive and negative control diets, respectively. The experimental diets' formulation, approximate composition and physical quality, in addition to their total and FAA composition, are presented in Tables 1-3.

Kousoulaki K., Rønnestad I., Rathore R., Sixten H.J., Campbell P., Nordrum S., Berge R.K, & Albrektsen S. (2018). Physiological responses of Atlantic salmon (Salmo salar L.) fed very low (3%) fishmeal diets supplemented with feeding-modulating crystalline amino acid mixes as identified in krill hydrolysate. Aquaculture (Amsterdam, Netherlands), 486.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!