Rtx5 database

Manufactured by Leco

The Rtx5 database is a collection of reference spectra and related data for use in analytical chemistry. It provides detailed information on the chemical and physical properties of a wide range of compounds, enabling identification and characterization of unknown samples.

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Lab products found in correlation

Chroma tof 4.3x software, by Leco (2 mentions)

2 protocols using rtx5 database

Comprehensive Analysis of Developmental Neurotoxicity

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The differences in toxic effects, morphological alterations related to developmental neurotoxicity, gene expression and metabolite change were analyzed using one-way analysis of variance (ANOVA) by SPSS 13.0 software (Chicago, IL, USA). The Chroma TOF4.3X software of LECO Corporation and LECO-Fiehn Rtx5 database were used for raw peak extracting, data baselines filtering and calibration of the baseline, peak alignment, deconvolution analysis, peak identification and integration of the peak area. The statistical significance was calculated using the Student t-test. Metabolic data were analyzed using R to screen for metabolic differences. Differences were considered statistically significant at P ≤ 0.05, after a fdr correction by “BH” method. R package “pheatmap” is used to draw heat maps. The free web-based tool MetaboAnalyst 4.0 uses a high-quality KEGG metabolic pathway (Danio rerio) database as a back-end knowledge base for pathway analysis and visualization (http://www.metaboanalyst.ca).

Zhu J., Wang C., Gao X., Zhu J., Wang L., Cao S., Wu Q., Qiao S., Zhang Z, & Li L. (2019). Comparative effects of mercury chloride and methylmercury exposure on early neurodevelopment in zebrafish larvae. RSC Advances, 9(19), 10766-10775.

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Metabolomic Analysis Using Chroma TOF4.3X

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Chroma TOF4.3X software by LECO Corporation and a LECO-Fiehn Rtx5 database was used to extract the raw peaks, filter and calibrate the baseline data, align the peaks, analyze the deconvolution, identify the peaks, and integrate the peak area. The retention time index (RI) was used for peak identification, and the RI tolerance was 5000. The difference between the metabolites in each group was calculated by finding the ratio of the mean value and transforming it with log₂. We set 1 as the threshold, log₂FC ≥ 1 as the content increased, −1 < log₂FC < 1 as the content was unchanged, and log₂FC ≤ −1 for the decrease. The heatmap was drawn with HemI software (version 1.0, http://hemi.biocuckoo.org/) [37 (link)]. The metabolic pathway map was drawn with reference to the KEGG metabolic database (http://www.genome.jp/kegg/).

Wang R., Lin K., Chen H., Qi Z., Liu B., Cao F., Chen H, & Wu F. (2021). Metabolome Analysis Revealed the Mechanism of Exogenous Glutathione to Alleviate Cadmium Stress in Maize (Zea mays L.) Seedlings. Plants, 10(1), 105.

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