Immunofluorescence staining was performed as previously described [45 (link)]. Cells were grown on coverslips until they reached 60–70% confluence. They were fixed in methanol for 10 minutes at -20°C, washed three times with DPBS (Dulbecco’s Phosphate-Buffered Saline, Thermo Fisher Scientific) and incubated overnight at 4°C with primary antibody Tomm20 (Novus Biologicals, NBP2-67501). After washing three times with DPBS, the specimens were incubated with Goat anti-Rabbit Alexa Flour 488 secondary antibody (Invitrogen, A-27034) for 1 hour at room temperature. All antibodies were diluted in 0.1% BSA in DPBS. Nuclear staining and mounting was performed with Vectashield mounting medium with DAPI (Vector Laboratories, CA, USA). Imaging was performed using a laser scanning confocal microscope (Zeiss LSM 880). All images were taken at 60x magnification with the same laser setting for comparative analysis between the experimental groups.
Goat anti rabbit alexa flour 488 secondary antibody
Manufactured by Thermo Fisher Scientific
Goat anti-Rabbit Alexa Flour 488 secondary antibody is a fluorescently labeled secondary antibody used to detect and visualize rabbit primary antibodies in various immunoassays and imaging applications.
Automatically generated - may contain errors
2 protocols using goat anti rabbit alexa flour 488 secondary antibody
1
Immunofluorescence Staining for Mitochondrial Protein
2
Immunofluorescence Staining of Mitochondrial Protein
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Immunofluorescence staining was performed as previously described 45 (link) . Cells were grown on coverslips until they reached 60 -70% confluence. They were fixed in methanol for 10 minutes at -20°C, washed three times with DPBS (Dulbecco's Phosphate-Buffered Saline, Thermo Fisher Scientific) and incubated overnight at 4°C with primary antibody Tomm20 (Novus Biologicals, NBP2-67501). After washing three times with DPBS, the specimens were incubated with Goat anti-Rabbit Alexa Flour 488 secondary antibody (Invitrogen, A-27034) for 1 hour at room temperature. All antibodies were diluted in 0.1 % BSA in DPBS. Nuclear staining and mounting was performed with Vectashield mounting medium with DAPI (Vector Laboratories, CA, USA). Imaging was performed using a laser scanning confocal microscope (Zeiss LSM 880). All images were taken at 60x magnification with the same laser setting for comparative analysis between the experimental groups.
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