The AQ knock-in mice were generated by transfecting the linearized targeting vector (Figure S1A ) into JM8A3.N1 C57BL/6N embryonic stem cells. Positive embryonic stem clone was subjected to the generation of chimera mice by injection using C57BL/6J blastocysts as the host. Successful germline transmission was confirmed by PCR sequencing (Figure S1B ). The heterozygous mice were bred to Actin-flpase mice [The Jackson Laboratory, B6.Cg-Tg(ACTFLPe)9205Dym/J] to remove the neo gene and make the AQ-MPYS knock-in mouse. Animals were generated at the National Jewish Health Mouse Genetics Core Facility.
Age- and gender-matched mice (8 – 52 weeks old, both male and female) were used for indicated experiments. HAQ (11 (link)) and MPYS−/− (16 (link)) mice were generated as previously described. Mice were housed at 22°C under a 12-h light-dark cycle with ad libitum access to water and chow diet (3.1 kcal/g, Teklad 2018, Envigo, Sommerset, NJ) and bred under pathogen-free conditions in the Animal Research Facility at the University of Florida. Littermates of the same sex were randomly assigned to experimental groups. All mouse experiments were performed by the regulations and approval of the Institutional Animal Care and Use Committee at the University of Florida, IACUC number 201909362.
Age- and gender-matched mice (8 – 52 weeks old, both male and female) were used for indicated experiments. HAQ (11 (link)) and MPYS−/− (16 (link)) mice were generated as previously described. Mice were housed at 22°C under a 12-h light-dark cycle with ad libitum access to water and chow diet (3.1 kcal/g, Teklad 2018, Envigo, Sommerset, NJ) and bred under pathogen-free conditions in the Animal Research Facility at the University of Florida. Littermates of the same sex were randomly assigned to experimental groups. All mouse experiments were performed by the regulations and approval of the Institutional Animal Care and Use Committee at the University of Florida, IACUC number 201909362.